| Project/Area Number |
19390285
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyushu University |
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Principal Investigator |
HARA Toshiro Kyushu University, 大学院・医学研究院, 教授 (40150445)
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| Co-Investigator(Kenkyū-buntansha) |
KUSUHARA Kouichi 九州大学, 医学研究院, 准教授 (20243941)
TAKADA Hidetoshi 九州大学, 大学病院, 准教授 (70294931)
IHARA Kenji 九州大学, 大学病院, 講師 (80294932)
NAKATSU Yoshimichi 九州大学, 医学研究院, 准教授 (00207820)
赤司 浩一 九州大学, 大学病院, 教授 (80380385)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 遺伝子治療 / 原発性免疫不全症候群 / 伴性劣性無ガンマグロブリン血症 / アデノAAVハイブリッドベクター / NOD / SCID / γc完全欠失マウス / B細胞分化障害 / ベクター / ヒト化マウス |
|---|
| Research Abstract |
We have made a HD-Ad.AAV.BTK vector containing BTK exon6～exon19, TIMM8A and GFP-Hyg. After treatment of a male pre-B ALL cell line, Nalm6, with the vector, Hyg-resistant clones were obtained. Recombination of BTK gene was demonstrated in the clones by Southern blotting. In addition, vector genome was integrated to CD34-positive cells from male cord blood.
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