Effects of brain niche constituents on differentiation and survival of neural cells containing oligodendrocytes
| Project/Area Number |
19390292
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Aichi Medical University |
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Principal Investigator |
OOHIRA Atsuhuko Aichi Medical University, 客員教授 (20101074)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANISHI Keiko 愛知県心身障害者コロニー, 発達障害研究所, 主任研究員 (50280813)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 未熟児 / 脳障害 / 子宮内胎児発育遅延 / 低酸素 / コンドロイチン硫酸 / 増殖因子 / オリゴデンドロサイト / 神経細胞 / 脳室周囲白質損傷 / 低出生体重 / 多動 / 神経幹細胞 / 虚血再灌流 / 学習障害 / ニューロカン / 脳周囲白質損傷(PWMI) / プロテオグリカン / 低酸素虚血 |
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| Research Abstract |
The final goal of our research is to conquer brain injuries often found in the premature newborn. As the experimental model, we produced fetal growth retardation (FGR) model rats. The FGR pups exhibited a significant delay in postnatal neurological development, a high density of apoptotic cells in the cortical plate, and a reduced amount of brain chondroitin sulfate proteoglycans that is a major brain niche constituent. Hypoxic treatment of a neural cell mixture which differentiated from neural stem cells in culture revealed that neuronal cells were more sensitive to hypoxia than oligodendrocytes. Addition of fibroblast growth factor-2 or epidermal growth factor to the hypoxic culture reduced cell death, suggesting that these growth factors are promising to prevent cell death found in the cortical plate of the FGR rat newborn.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] A highly sulfated chondroitin sulfate preparation, CS-E, prevents excitatory amino acid-induced neuronal cell death.2008
Author(s)
Yoshiaki Sato, Keiko Nakanishi, Yoshihito Tokita, Hiroko Kakizawa, Michiru Ida, Hiroshi Maeda, Fumiko Matsui, Sachiko Aono, Akiko Saito, Yoshiyuki Kuroda, Masahiro Hayakawa, Seiji Kojima, Atsuhiko Oohira
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Journal Title
Journal of Neurochemistry Vol.104,No.6
Pages: 1565-1576
Related Report
Peer Reviewed
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[Journal Article] Reduction of brain injury in neonatal hypoxic-ischemic rats by intracerebroventricular injection of neural stem/progenitor cells together with chondroitinase ABC.2008
Author(s)
Yoshiaki Sato, Keiko Nakanishi, Masahiro Hayakawa, Hiroko Kakizawa, Akiko Saito, Yoshiyuki Kuroda, Michiru Ida, Yoshihito Tokita, Sachiko Aono, Fumiko Matsui, Seiji Kojima, Atsuhiko Oohira
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Journal Title
Reproductive Sciences Vol.15,No.6
Pages: 613-620
Related Report
Peer Reviewed
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[Journal Article] Ectodomain shedding of neuroglycan C, a brain-specific chondroitin sulfate proteoglycan, by TIMP-2- and TIMP-3-sensitive proteolysis.2007
Author(s)
Takuya Shuo, Sachiko Aono, Keiko Nakanishi, Yoshihito Tokita, Yoshiyuki Kuroda, Michiru Ida, Fumiko Matsui, Hiroko Maruyama, Toshiyuki Kaji, Atsuhiko Oohira
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Journal Title
Journal of Neurochemistry Vol.102,No.5
Pages: 1561-1568
Related Report
Peer Reviewed
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