| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Research Abstract |
Creation of the highly functional three dimensional (3-D) tissue with microvascular networks has been eagerly waited for regenerative medicine, however, the technology of such reconstitution is not established enough. The final goal of our study is creation of human highly functional and complicated tissues. To reach this final goal, we first seeded human umbilical-vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSC) in a three-dimensional fibronectin-collagen gel and reconstituted human 3D microvessels in vitro. Next, we reconstituted this human microvessls in vivo by transplanting a small piece of this fibronectin-collagen gel embedded HUVEC and hMSC in mouse cranial windows and aimed at the creation of human 3D hepatic tissue with microvascular network.
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