Engineering three dimensional hepatic tissue with microvascular networks in vivo
| Project/Area Number |
19390340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOIKE Naoto Yokohama City University, 医学研究科, 客員研究員 (50301081)
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| Co-Investigator(Kenkyū-buntansha) |
谷口 英樹 横浜市立大学, 医学研究科, 教授 (70292555)
鄭 允文 横浜市立大学, 医学部, 助教 (80404995)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 移植外科学 / 人工微小血管 / 三次元培養 / 人工肝 / 微小人工血管 / クラニアルウィンドウ / 生体顕微鏡 / 血管 / 肝臓 / 組織再構築 / 再生医学 |
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| Research Abstract |
Creation of the highly functional three dimensional (3-D) tissue with microvascular networks has been eagerly waited for regenerative medicine, however, the technology of such reconstitution is not established enough. The final goal of our study is creation of human highly functional and complicated tissues. To reach this final goal, we first seeded human umbilical-vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSC) in a three-dimensional fibronectin-collagen gel and reconstituted human 3D microvessels in vitro. Next, we reconstituted this human microvessls in vivo by transplanting a small piece of this fibronectin-collagen gel embedded HUVEC and hMSC in mouse cranial windows and aimed at the creation of human 3D hepatic tissue with microvascular network.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] Intraductal tubulopapillary neoplasms of the pancreas distinct from pancreatic intraepithelial neoplasia and intraductal papillary mucinous neoplasms.2009
Author(s)
Yamaguchi H, Shimizu M, Ban S, Koyama I, Hatori T, Fujita I, Yamamoto M, Kawamura S, Kobayashi M, Ishida K, Morikawa T, Motoi F, Unno M, Kanno A, Satoh K, Shimosegawa T, Orikasa H, Watanabe T, Nishimura K, Ebihara Y, Koike N, Furukawa T
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Journal Title
Am J Surg Pathol. 33(8)
Pages: 1164-1172
Related Report
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[Journal Article] Intraductal tubulopapillary neoplasms of the pancreas distinct from pancreatic intraepithelial neoplasia and intraductal papillary mucinous neoplasms2009
Author(s)
Yamaguchi H, Shimizu M, Ban S, Koyama I, Hatori T, Fujita I, Yamamoto M, Kawamura S, Kobayashi M, Ishida K, Morikawa T, Motoi F, Unno M, Kanno A, Satoh K, Shimosegawa T, Orikasa H, Watanabe T, Nishimura K, Ebihara Y, Koike N, Furukawa T
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Journal Title
Am J Surg Pathol 33
Pages: 1164-1172
Related Report
Peer Reviewed
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[Journal Article] Hepatic hilar resection for hilar cholangiocarcinoma based on a reclassification.2008
Author(s)
Cho A, Asano T, Yamamoto H, Nagata M, Takiguchi N, Kainuma O, Souda H, Gunji H, Miyazaki A, Nojima H, Ikeda A, Matsumoto I, Ryu M, Koike N
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Journal Title
Hepatogastroenterology 55(86-87)
Pages: 1764-1765
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[Journal Article] The polycomb gene product BMI1 contributes to the maintenance of tumor-initiating side population cells in hepatocellular carcinoma.2008
Author(s)
Chiba T, Miyagi S, Saraya A, Aoki R, Seki A, Morita Y, Yonemitsu Y, Yokosuka O, Taniguchi H, Nakauchi H, Iwama A
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Journal Title
Cancer Research 68(19)
Pages: 7742-9
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[Journal Article] Hepatic hilar resection for hilar cholangiocarcinoma based on a reclassification.2008
Author(s)
Cho A, Asano T, Yamamoto H, Nagata M, Takiguchi N, Kainuma O, Souda H, Gunji H, Miyazaki A, Nojima H, Ikeda A, Matsumoto I, Ryu M, Koike N
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Journal Title
Hepatogastroenterology 55
Pages: 1764-1765
Related Report
Peer Reviewed
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[Journal Article] Intersegmental arterial communication between the medial and left lateral segments of the liver.2007
Author(s)
Cho A, Gunji H, Koike N, Narumoto S, Asano T, Yamamoto H, Kainuma O, Ryu M, Mori C, Murakami G, Okazumi S, Ochiai T
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Journal Title
Dig Surg 24(5)
Pages: 328-30
Related Report
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[Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation.2007
Author(s)
Chiba T, Zhen YW, Kita K, Yokosuka O, Saisho H, Onodera M, Miyoshi H, Nakano M, Zen Y, Nakanuma Y, Nakauchi H, Iwama A, Taniguchi H
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Journal Title
Gastroenterology 133
Pages: 937-950
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[Journal Article] Renal L-Type Fatty Acid-Binding Protein in Acute Ischemic Injury2007
Author(s)
Yamamoto T, Noiri E, Ono Y, Doi K, Negishi K, Kamijo A, Kimura K, Fujita T, Kinukawa T, Taniguchi H, Nakamura K, Goto M, Shinozaki N, Ohshima S, Sugaya T
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Journal Title
Journal of the American Society of Nephology 18
Pages: 2894-2902
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[Journal Article] Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules.2007
Author(s)
Kita K, Watanabe T, Ohsaka K, Hayashi H, Kubota Y, Nagashima Y, Aoki I, Taniguchi H, Noce T, Inoue K, Miki H, Ogonuki N, Tanaka H, Ogura A, Ogawa T
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Journal Title
Biol Reprod. 76(2)
Pages: 211-7
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[Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation2007
Author(s)
Chiba T, Zhen YW, Kita K, Yokosuka O, Saisho H, Onodera M, Miyoshi H, Nakano M, Zen Y, Nakanuma Y, Nakauchi H, Iwama A, Taniguchi H
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Journal Title
Gastroenterology 133
Pages: 937-950
Related Report
Peer Reviewed
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[Presentation] In vivoにおける血管ネットワーク構造を有するヒト高次肝像組織の再構築.2009
Author(s)
仲野晶, 藤原綾二, 小池直人, 千葉豊生, 網谷猛, 岡村愛, Dai Fukumura, Rakesh K Jain, 上野康晴, 鄭允文, 谷口英樹
Organizer
第8回日本再生医療学会総会
Place of Presentation
東京
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