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Molecule targeted therapy for Cholangiocarcinoma based on the gene profiling of cancer stem cell

Research Project

Project/Area Number 19390348
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNagoya University

Principal Investigator

NAGINO Masato  Nagoya University, 医学系研究科, 教授 (20237564)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Yukihiro  名古屋大学, 大学院・医学系研究科, 寄附講座講師 (80378091)
KOKURYO Toshio  名古屋大学, 大学院・医学系研究科, 特任助教 (60378023)
安部 哲也  愛知県がんセンター中央病院, 胸部外科部, 医長 (90378092)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords胆管癌幹細胞 / 分子標的治療 / 肝門部胆管癌
Research Abstract

Cancer stem cell, have the ability of differentiation and self-reproduction, is important in carcinogenesis and cancer progression.
We analyzed a gene profiling between CD133 positive HuCCT1 and the CD133 negative HuCCT1 by DNA array. As a result, we were not able to find the relationship between CD133 and hepatic stem cell marker, such as CD45, TER, CD49f, and c-Met.
And then, we inoculated CD133 positive HuCCT1 and the CD133 negative HuCCT1 in the nude mouse. There was significant difference of carcinogenesis in this model. We considered that this caused in various factors, such as the cancer origin, another cancer stem cell marker except CD133.
In addition, we administered Nek2 siRNA in liver metastasis of a nude rat through portal vein using the access port. We recognized decrease of the number of the liver metastasis and the reduction of the tumor volume.
We clarified that the drug delivery system of siRNA through the portal vein was effective.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2010 2009 2007

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (3 results)

  • [Journal Article] Hepatectomy with simultaneous resection of the portal vein and hepatic artery for advanced perihilar cholangiocarcinoma : an audit of 50 consecutive cases.2010

    • Author(s)
      Nagino M, Nimura Y, Kamei Y., (他7名)
    • Journal Title

      Ann Surg. 252(1)

      Pages: 115-123

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Value of indocyanine green clearance of the future liver remnant in predicting outcome after resection for biliary cancer.2010

    • Author(s)
      Yokoyama Y, Sugawara G, Nagino M., (他5名)
    • Journal Title

      Br J Surg. 97(8)

      Pages: 1260-1268

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 15-deoxy-delta 12,14-prostaglandin J2 prevents inflammatory response and endothelial cell damage in rats with acute obstructive cholangitis.2010

    • Author(s)
      Watanabe K, Yokoyama Y, Nagino M., (他7名)
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol. 298(3)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Silencing of Tousled-like kinase 1 sensitizes cholangiocarcinoma cells to cisplatin-induced apoptosis.2010

    • Author(s)
      Takayama Y, Kokuryo T, Senga T., (他6名)
    • Journal Title

      Cancer Lett. 296(1)

      Pages: 27-34

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel combination treatment for colorectal cancer using Nek2 siRNA and cisplatin.2010

    • Author(s)
      Suzuki K, Kokuryo T, Hamaguchi M., (他5名)
    • Journal Title

      Cancer Sci. 101(5)

      Pages: 1163-1169

    • NAID

      10026592095

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inchinkoto, an herbal medicine, exerts beneficial effects in the rat liver under stress with hepatic ischemia-reperfusion and subsequent hepatectomy.2010

    • Author(s)
      Kawai K, Kokuryo T, Nagino M., (他5名)
    • Journal Title

      Ann Surg. 251(4)

      Pages: 692-700

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Silencing of Tousled-like kinase 1 sensitizes cholangiocarcinoma cells to cisplatin-induced apoptosis.2010

    • Author(s)
      Takayama Y, Kokuryo T, Nagino M., 他6名
    • Journal Title

      Cancer Lett.296(1):27-34.2010

      Volume: 296(1) Pages: 27-34

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel combination treatment for colorectal cancer using Nek2 siRNA and cisplatin.2010

    • Author(s)
      Suzuki K, Kokuryo T, Nagino M
    • Journal Title

      Cancer Sci.

      Volume: 101(5) Pages: 1163-1169

    • NAID

      10026592095

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatectomy with simultaneous resection of the portal vein and hepatic artery for advanced perihilar cholangiocarcinoma : an audit of 50 consecutive cases.2010

    • Author(s)
      Nagino M, Nimura Y, Kamei Y., 他7名
    • Journal Title

      Ann Surg.

      Volume: 252(1) Pages: 115-123

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathologic study of cholangiocarcinoma with superficial spread.2009

    • Author(s)
      Igami T, Nagino M, Yokoyama Y, (他4名)
    • Journal Title

      Ann Surg. 249(2)

      Pages: 296-302

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nek2 as a novel molecular target for the treatment of breast carcinoma.2009

    • Author(s)
      Tsunoda N, Kokuryo T, Nagino M., (他4名)
    • Journal Title

      Cancer Sci. 100(1)

      Pages: 111-116

    • NAID

      10025141544

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Apoptosis induction by antisense oligonucleotides against miR-17-5p and miR-20a in lung cancers overexpressing miR-17-92.2007

    • Author(s)
      Matsubara H, Nagino M, Nimura Y, (他9名)
    • Journal Title

      Oncogene 26(41)

      Pages: 6099-6105

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] CLCP1 interacts with semaphoring 4B and regulates motility of lung cancer cells.2007

    • Author(s)
      Nagai H, Nagino M, Nimura Y, (他7名)
    • Journal Title

      Oncogene 26

      Pages: 4025-4031

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of impaired hepatic regeneration in cholestatic liver.2007

    • Author(s)
      Yokoyama Y, Nagino M, Nimura Y.
    • Journal Title

      J Hepatobiliary Pancreat Surg. 14

      Pages: 159-166

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances in treatment of hilar cholangiocarcinoma : Portal vein embolization.2007

    • Author(s)
      Yokoyama Y, Nagino M, Nimura Y., (他5名1)
    • Journal Title

      J HPB Surg. 14

      Pages: 447-454

    • NAID

      10019811065

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Is there any effect of renal failure on the hepatic regeneration capacity following partial hepatectomy in rats?2007

    • Author(s)
      Kawai T, Yokoyama Y, Nagino M, (他2名)
    • Journal Title

      Biochem Biophys Res Commun. 352

      Pages: 311-316

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nek2 as an affective target for inhibition of tumorigenic growth and peritoneal dissemination of cholangiocarcinoma.2007

    • Author(s)
      Kokuryo T, Yokoyama Y, Nagino M, (他3名)
    • Journal Title

      Cancer Research 67(20)

      Pages: 9637-9642

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] siRNAを用いた癌分子標的治療のトランス2010

    • Author(s)
      國料俊男、横山幸浩、梛野正人
    • Organizer
      レーショナルリサーチJDDW2010
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Nek2 as an effective molecular target for cancer treatment2010

    • Author(s)
      Kokuryo T, Senga T, Yokoyama Y, Nagino M, Hamaguchi M.
    • Organizer
      AACR 101^<st> annual meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] siRNAを用いた癌分子標的治療のトランスレーショナルリサーチ2010

    • Author(s)
      國料俊男、横山幸浩、梛野正人
    • Organizer
      JDDW 2010
    • Place of Presentation
      横浜
    • Related Report
      2010 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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