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The expression and function of microRNA in esophageal cancer cells and normal esophageal epithelial cells

Research Project

Project/Area Number 19390356
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Toyama

Principal Investigator

SHIMADA Yutaka  University of Toyama, 大学院・医学薬学研究部, 准教授 (30216072)

Co-Investigator(Kenkyū-buntansha) TSUJIMOTO Gozoh  京都大学, 大学院・薬学研究科, 教授 (80172013)
SHINODA Masayuki  愛知県立がんセンター中央病院, 病院長 (00416166)
TSUKADA Kazuhiro  富山大学, 大学院・医学薬学研究部, 教授 (90171967)
FUJIMOTO Jiro  兵庫医科大学, 医学部, 教授 (90199373)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords食道外科 / 食道癌 / 正常食道上皮 / miRNA / PLK-1 / miR-593* / miR-106b-25 / polycistron / 食道腺癌 / miR-106b-25 polycistron / 食道癌細胞株 / real time PCR / マイクロアレイ解析 / 薬剤感受性 / CDDP / 5Fu
Research Abstract

正常食道上皮で発現が見られないPolo-like kinase 1 (PLK1)が食道扁平上皮癌細胞株11株で発現しており、このPLK1をターゲットとするmiR-593*を同定した。一方、Barrett腺癌ではmiR-106b-25クラスターが高発現しており、miRs -93 と -106b のターゲットはp21で、miR-25 のターゲットはBimと考えられた。以上の事から食道扁平上皮癌ではmiR-593*が、腺癌ではmiR-106b-25クラスターが食道癌における有力な分子標的となりうるものと考えられた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 10 results) Presentation (13 results) Book (2 results)

  • [Journal Article] cDNA microarray analysis of esophageal cancer : Discoveries and prospects2009

    • Author(s)
      Shimada Y , Tsujimoto G et.al.
    • Journal Title

      Gen Thorac Cardiovasc Surgery (In press)

    • NAID

      10025151661

    • Related Report
      2008 Final Research Report
  • [Journal Article] ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis2009

    • Author(s)
      Iriyama T, Shimada Y et.al.
    • Journal Title

      EMBO Journal advance online publication

    • Related Report
      2008 Final Research Report
  • [Journal Article] cDNA microarray analysis of esophageal cancer : Discoveries and prospects2009

    • Author(s)
      Shimada Y
    • Journal Title

      Gen Thorac Cardiovasc Surgery 57(In press)

    • NAID

      10025151661

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis2009

    • Author(s)
      Iriyama T
    • Journal Title

      EMBO J (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PTTG1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma2008

    • Author(s)
      Ito T, Shimada Y et.al.
    • Journal Title

      Cancer Res 68

      Pages: 3214-3224

    • Related Report
      2008 Final Research Report
  • [Journal Article] Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma (ESCC) and its clinical significance2008

    • Author(s)
      Ortiz C, Shimada Y, et.al.
    • Journal Title

      Int J Cancer 122

      Pages: 325-332

    • Related Report
      2008 Final Research Report
  • [Journal Article] Global correlation analysis for micro-RNA and mRNA expression profiles in human cell lines2008

    • Author(s)
      Ruike Y, Tsujimoto G, et.al.
    • Journal Title

      J Hum Genet 53

      Pages: 515-23

    • Related Report
      2008 Final Research Report
  • [Journal Article] PTTG1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma2008

    • Author(s)
      Ito T
    • Journal Title

      Cancer Res 68

      Pages: 3214-3224

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global correlation analysis for micro-RNA and mRNA expression profiles in human cell lines.2008

    • Author(s)
      Ruike Y
    • Journal Title

      J Hum Genet 53

      Pages: 515-523

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma (ESCC) and its clinical significance2008

    • Author(s)
      Ortiz C
    • Journal Title

      Int J cancer 122

      Pages: 325-332

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ultrasensitive new DNA microarray chip provides gene expression profiles forpreoperative esophageal cancer biopsies without RNA amplification2008

    • Author(s)
      Ito T
    • Journal Title

      Oncology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma(ESCC)and its clinical significance2008

    • Author(s)
      Ortiz C
    • Journal Title

      Int J Cancer 122

      Pages: 325-332

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ultrasensitive new DNA microarray chip provides gene expression profiles for preoperative esophageal cancer biopsies without RNA amplification2007

    • Author(s)
      Ito T, Tsujimoto G, Shimada Y, et.al.
    • Journal Title

      Oncology 73

      Pages: 366-375

    • Related Report
      2008 Final Research Report
  • [Journal Article] Molecular factors related to metastasis of esophageal squamous cell carcinoma2007

    • Author(s)
      Shimada Y and Sato F
    • Journal Title

      Esophagus 4

      Pages: 7-18

    • Related Report
      2008 Final Research Report
  • [Journal Article] The suppression of Aurora-A/STK15/BTAK expression enhances chemo-sensitivity to Docetaxel in human esophageal squamous cell carcinoma2007

    • Author(s)
      Tanaka E, Shimada Y, et.al.
    • Journal Title

      Clin Cancer Res 13

      Pages: 1331-1340

    • Related Report
      2008 Final Research Report
  • [Journal Article] Molecular factors related to metastasis of esophageal squamous cell carcinoma2007

    • Author(s)
      Shimada Y
    • Journal Title

      Esophagus 4

      Pages: 7-18

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The suppression of Aurora-A/STK15/BTAK expression enhances chemo-sensitivityto Docetaxel in human esophageal squamous cell carcinoma2007

    • Author(s)
      Tanaka E
    • Journal Title

      Clin Cancer Res 13

      Pages: 1331-1340

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABCG2 Expression is an independent Unfavorable Prognostic Factor in Esophageal Squamous Cell Carcinoma2007

    • Author(s)
      Tsunoda S
    • Journal Title

      Oncology 71

      Pages: 251-258

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Polo-like kinase 1の食道癌細胞増殖能に於ける役割とmicroRNA-593*による転写後制御2008

    • Author(s)
      佐藤史顕、土屋創建、嶋田裕、清水一治
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Polo-like kinase 1の食道癌細胞増殖能に於ける役割とmicro RNA-593*による転写後制御.2008

    • Author(s)
      佐藤史顕
    • Organizer
      第67回日本癌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Predicting lymph node metastasis by DNA microarray analysis of preoperative endoscopic biopsy specimens from esophageasl cancer patients2008

    • Author(s)
      Shimada Y, Shinoda M, Kato K, Tsujimoto G
    • Organizer
      UICC World Cancer Congress
    • Place of Presentation
      Geneva
    • Year and Date
      2008-08-30
    • Related Report
      2008 Final Research Report
  • [Presentation] Comprehensive analysis of microRNA target networks.2008

    • Author(s)
      Tsujimoto G
    • Organizer
      The Uehara Memorial Foundation Symposium-2008
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-06-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] Down-regulation of PTTG1 by siRNA suppresses tumorigenesis and lymph node metastasis of esophageal squamous cell carcinoma in vivo2008

    • Author(s)
      Ito T, Shimada Y, et.al.
    • Organizer
      109th annual meeting of the AGA institute
    • Place of Presentation
      San Diego
    • Year and Date
      2008-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Fascin modifies the expression of c-erbB-2/HER-2 and beta -catenin in the esophageal squamous cell carcinoma (ESCC) 20082008

    • Author(s)
      Ortiz C, Shimada Y, et.al.
    • Organizer
      annual meeting of AACR
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-14
    • Related Report
      2008 Final Research Report
  • [Presentation] Down-regulation of Polo-like kinase 1 by siRNA drastically suppresses proliferation of esophageal cancer cells2008

    • Author(s)
      Ito T, Shimada Y, et.al.
    • Organizer
      2008 annual meeting of AACR
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Significance of Karyopherin alfa 2 (KPNA2) expression in esophageal squamous cell carcinoma2008

    • Author(s)
      Shimada Y, et.al.
    • Organizer
      2008 annual meeting of AACR
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-12
    • Related Report
      2008 Final Research Report
  • [Presentation] Comprehensive analysis of microRNA target networks2008

    • Author(s)
      Tsujimoto G, Okuno Y, Terasawa K, Tuchiya S
    • Organizer
      The Uehara Memorial Foundation Symposium-2008
    • Place of Presentation
      Tokyo Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] SiRNA treatment for esophageal cancer cells2007

    • Author(s)
      Shimada Y, Fujimoto J, et.al.
    • Organizer
      42nd Congress of the European Surgical Research (ESSR)
    • Place of Presentation
      Rotterdam
    • Year and Date
      2007-05-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Downregulation of PTTG1 by SiRNA supress cell proliferation of esophageal squamous cell carcinoma2007

    • Author(s)
      Ito T, Shimada Y, et.al.
    • Organizer
      2007 Annual meeting of AACR
    • Place of Presentation
      Los Angeles
    • Year and Date
      2007-04-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Role of Fascin in anoikis and tumor formation in esophageal squamous cell carcinoma (ESCC).2007

    • Author(s)
      Ortiz C, Shimada Y, et.al.
    • Organizer
      2007 Annual meeting of AACR
    • Place of Presentation
      Los Angeles
    • Year and Date
      2007-04-16
    • Related Report
      2008 Final Research Report
  • [Presentation] Prognostic significance of PTTG1/securin in esophageal cancewr patients2007

    • Author(s)
      Shimada Y, Fujimoto J, et.al.
    • Organizer
      2007 Annual meeting of AACR
    • Place of Presentation
      Los Angeles
    • Year and Date
      2007-04-15
    • Related Report
      2008 Final Research Report
  • [Book] MicroRNAs and discovery of new targets. Genomics and Pharmacogenomics in Anticancer Drug Development and Clinical Response2008

    • Author(s)
      Tsuchiya S
    • Total Pages
      377
    • Publisher
      Humana Press, Totowa, NJ. In Innocenti, F. (ed. )
    • Related Report
      2008 Annual Research Report
  • [Book] MicroRNAs and discovery of new targets. In Innocenti, F. (ed.), Genomics and Pharmacogenomics in Anticancer Drug Development and Clinical Response

    • Author(s)
      Tsuchiya S., Okuno Y. and Tsujimoto G
    • Publisher
      Humana Press
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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