Development of genetic diagnosis and drugs for lung cancer, based on protein phosphatase targeting

• Project/Area Number: 19390370
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Miyagi Cancer Center Research Institute
• Principal Investigator: SATO Masami Miyagi Cancer Center Research Institute, 薬物療法学部, 特任研究員 (30250830)
• Co-Investigator(Kenkyū-buntansha): SHIMA Hiroshi 宮城県立がんセンター研究所, 薬物療法学部, 部長 (10196462) ; TANUMA Nobuhiro 宮城県立がんセンター研究所, 薬物療法学部, 研究員 (40333645) ; NOMURA Miyuki 宮城県立がんセンター研究所, 薬物療法学部, 技師 (40390893) ; SASAKI Nozomi 宮城県立がんセンター研究所, 薬物療法学部, 臨時職員 (50467429)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 呼吸器外科学 / プロテインホスファターゼ / スプライシング / 細胞内トランスポート / KIF3モーター / MAPK経路 / 遺伝子発現異常 / 遺伝子変異 / 肺癌 / 前癌病変 / 早期肺癌 / HRM / 組織バンク

Research Abstract

(1) Regulation of mRNA splicing, intracellular transport using KID3 motor, and ERK activation by protein phosphatases has been revealed.
(2) Expression of protein phosphatase genes in lung tumor tissues has been analyzed.

Research Products

• [Journal Article] MKP-7, a JNK phosphatase, blocks ERK-dependent gene activation by anchoring phosphorylated ERK in the cytoplasm. (2010)
  • Author(s): Masuda K, Katagiri C, Nomura M, Sato M, Kakumoto K, Akagi T, Kikuchi K, Tanuma N, Shima H.
  • Journal Title: Biochem Biophys Res Commun 393 Pages: 201-206
• [Journal Article] NKP-7, a JNK phosphatase, blocks ERK-dependent gene activation by anchoring phosphorylated ERK in the cytoplasm (2010)
  • Author(s): Masuda K, Sato M, et al
  • Journal Title: Biochem Biophys Res Commun 393 Pages: 201-206
  • Peer Reviewed
• [Journal Article] Protein phosphatase Dusp26 associates with KIF3 motor and promotes N-cadherin-mediaded cell-cel adhesion. (2009)
  • Author(s): Tanuma N, Nomura M, Ikdea M, Kusugai I, Tsubaki Y, Takagaki K, Kawamura T, Yamashita Y, Sato I, Sato M, Katakura R, Kikuchi K, Shima H
  • Journal Title: Oncogene 28 Pages: 752-76
• [Journal Article] Four year experience of the survey on quality control of lung cancer screening system in Japan. (2009)
  • Author(s): Sagawa M, Endo C, Sato M, Saito Y, Sobue T, Usuda K, Aikawa H, Fujimura S, Sakuma T
  • Journal Title: Lung Cancer 63 Pages: 291-294
• [Journal Article] Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung (2009)
  • Author(s): Endo C, Honda M, Sakurada A, Sato M, Saito Y, Kondo T
  • Journal Title: Acta Cytol 53 Pages: 36-40
• [Journal Article] Protein phosphatase Dusp26 associates with KIF3 motor and promotes N-cadherin-mediated cell-cell adhesion (2009)
  • Author(s): Tanuma N, Sato M, et al.
  • Journal Title: Oncogene 28巻 Pages: 752-761
  • Peer Reviewed
• [Journal Article] Four year experience of the survey on quality control of lung cancer screening system in Japan (2009)
  • Author(s): Sagawa M, Sato M, et al.
  • Journal Title: Lung Cancer 63巻 Pages: 291-294
  • Peer Reviewed
• [Journal Article] 胸部外科の指針。多形性癌13例の検討に対する討論1 (2009)
  • Author(s): 佐藤雅美
  • Journal Title: 胸部外科 62巻 Pages: 93-94
  • Peer Reviewed
• [Journal Article] Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung (2009)
  • Author(s): Endo C, Sato M, et al.
  • Journal Title: Acta Cytol 53巻 Pages: 36-40
  • Peer Reviewed
• [Journal Article] Results of long-term follow-up of photodynamic therapy for roentgenographically occult bronchogenic squamous cell carcmoma (2009)
  • Author(s): Endo C, Sato M, et al.
  • Journal Title: Chest 136巻 Pages: 369-375
  • Peer Reviewed
• [Journal Article] siRNA targeting against EGFR, a promising candidate for a novel therapeutic application to lung adenocarcinoma Pathobiology (2008)
  • Author(s): Yamanaka S, Gu Z, Sato M, Fujisaki R, Inomata K, Sakurada A, Inoue A, Nukiwa T, Kondo T, Horii A
  • Journal Title: 75 Pages: 2-8
• [Journal Article] The methylation status of FBXW7 beta-form correlates with histological subtype in human thymoma, Biochem Biophys Res Commun (2008)
  • Author(s): Gu Z, Mitsui H, Inomata K, Honda M, Endo C, Sakurada A, Sato M, Okada Y, Kondo T, Horii A
  • Journal Title: 377 Pages: 685-688
• [Journal Article] Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1(PP1) to dephosphorylate the U2 small nuclesr ribonucleoprotein particle(snRNP)component (2008)
  • Author(s): Tanuma N, Kim SE, Buellens M, Tsubaki Y, Mitsuhashi S, Nomura M, Kawamura T, Isono K, Koseki H, Sato M, Bollen M, Kikuchi K, Shima H
  • Journal Title: spliceosome-associated protein 155 (Sap155). J Biol Chem 52 Pages: 35805-35814
• [Journal Article] siRNA targeting against EGFR (2008)
  • Author(s): Yamanaka S, Gu Z, Sato M, Fujisaki R, Inomata K, Sakurada A, Inoue A, Nukiwa T, Kondo T, Horii A
  • Journal Title: a promising candidate for a novel therapeutic application to lung adenocarcinoma. Pathobiology 75(1) Pages: 2-8
• [Journal Article] Safety Study of Endobronchial Electrosurgery for Tracheobronchial Lesions Multicenter Prospective Study (2008)
  • Author(s): Horinouchi H
  • Journal Title: J Bronchol 15 Pages: 228-232
  • Peer Reviewed
• [Journal Article] Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 snRNP component, spliceosome-associated protein 155 (Sap155) (2008)
  • Author(s): Tanuma N
  • Journal Title: J Biol Chem 283 Pages: 35805-35814
  • Peer Reviewed
• [Journal Article] The methylation status of FBXW7 beta-form correlates with histological subtype in human thymoma (2008)
  • Author(s): Gu Z
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 685-688
  • Peer Reviewed
• [Journal Article] 細胞検査士の視機能および身体的不定愁訴に関する疫学的検討-経時的検討を含めて- (2008)
  • Author(s): 金城満
  • Journal Title: 日本臨床細胞学会雑誌 47 Pages: 407-415
  • Peer Reviewed
• [Journal Article] siRNA targeting against EGFR,a promising candidate for a novel therapeutic application to lung adenocarcinoma (2008)
  • Author(s): Sumikata Yamanaka
  • Journal Title: Pathology 75 Pages: 2-8
  • Peer Reviewed
• [Journal Article] The NMR structure of the NIPP1 FHA domain. (2008)
  • Author(s): Hiroyuki Kumeta
  • Journal Title: J Biomol NMR 40 Pages: 219-224
  • Peer Reviewed
• [Journal Article] Characterization of novel low-molecular-mass dual specificity phosphatase-4(LDP-4)expressed in brain (2007)
  • Author(s): Kentaro Takagaki
  • Journal Title: Molecular and Cellular Biochemistry 296 Pages: 177-184
  • Peer Reviewed
• [Presentation] 胸腺原発MALTリンパ腫の1切除例 (2009)
  • Author(s): 阿部二郎
  • Organizer: 日本肺癌学会東北支部会
  • Place of Presentation: 郡山市
  • Year and Date: 2009-08-01
• [Presentation] Dusp26 Associates with KIF3 Motor and Promotes Cadherin-Mediated Cell-Cell Adhesion EUROPHOSPHATASE 2009 (2009)
  • Author(s): Miyuki Nomura, Nobuhiro Tanuma, Isao Kasugai, Masami Sato, Hiroshi Shima
  • Organizer: Protein phosphatases in development and disease, Egmond aan Zee
  • Place of Presentation: The Netherlands
  • Year and Date: 2009-07-16
• [Presentation] 私のこだわる肺癌診療-肺癌 en-block resection, 気管支OCT, 肺癌検診- (2009)
  • Author(s): 佐藤雅美
  • Organizer: 鹿児島呼吸器学術講演会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2009-06-19
• [Presentation] シンポジウム2、気管支鏡による肺門部画像診断の最先端、Optical biopsyの時代へ-OCTによる上皮層から軟骨層までの画像観察- (2009)
  • Author(s): 佐藤雅美、高橋里美、前田寿美子、阿部二郎、前門戸任、松原信行
  • Organizer: 第32回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-29
• [Presentation] 気管支鏡による肺門部画像診断の最先端、Optical biopsy の時代へ-OCTによる上皮層から軟骨層までの画像観察 (2009)
  • Author(s): 佐藤雅美
  • Organizer: 日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-29
• [Presentation] 当院におけるコンベックス型走査式超音波気管支鏡ガイド下針生検(EBUS=TBNA)の診断成績 (2009)
  • Author(s): 松原信行
  • Organizer: 日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-29
• [Presentation] 上縦隔リンパ節を肺葉と連続せしめたまま一塊として摘出する左上葉切除術 (2009)
  • Author(s): 佐藤雅美
  • Organizer: 日本呼吸器外科学会総会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-05-15
• [Presentation] 喀痰細胞診による肺癌検診25年に学ぶ (2008)
  • Author(s): 佐藤雅美
  • Organizer: 第49回日本臨床細胞学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-07
• [Presentation] 肺切除術後における血清トロンボモジュリン値の変化 (2008)
  • Author(s): 前田寿美子、野津田泰嗣、高橋里美、小池加保児、佐藤雅美
  • Organizer: 第25回日本呼吸器外科学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 2008-05-29
• [Presentation] Trends in incidence of lung cancer by histological type in Miyagi (2008)
  • Author(s): Yoshikazu Nishino, Masami Sato, Yuko Minami, Ichiro Tsuji
  • Organizer: Japan IACR meeting
  • Place of Presentation: Sydney
• [Presentation] マレイン酸イルソグラジンを用いた原発性肺癌完全切除術後治療の無作為化比較試験 (2007)
  • Author(s): 高橋里美、佐藤雅美、一ノ瀬高志、佐川元保、遠藤千顕、佐藤徹、鈴木弘行、千田雅行、佐久間勉、佐藤信之、谷田達男、中村好宏、羽隅透、菅野隆三、近藤丘
  • Organizer: 第48回日本肺癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-11-09
• [Presentation] 肺癌手術においてリンパ節を肺葉とともにen-blockとして切除することはどこまで可能か? (2007)
  • Author(s): 佐藤 雅美
  • Organizer: 第48回日本肺癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-11-09
• [Book] 呼吸器症候群(第2版)III-その他の呼吸器疾患も含めて-VIII腫瘍性疾患、E. その他の腫瘍性病変、重複癌
  • Author(s): 佐藤雅美, 他
  • Publisher: 日本臨床社
• [Remarks]
  • URL: http://www.pref.miyagi.jp/mcc/