AAV vector-mediated cell and gene therapy for neuronal diseases
| Project/Area Number |
19390383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
OKADA Takashi National Center of Neurology and Psychiatry, 神経研究所・遺伝子疾患治療研究部, 室長 (00326828)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | AAVベクター / 遺伝子治療 / 神経疾患 / 神経筋疾患 |
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| Research Abstract |
To realize novel protein supplementation therapy for neuronal diseases, we have developed systems for AAV vector production and vector-producing cells. By using effective ion-exchange adsorbers, improved purification of vector particles was investigated. In addition to the step-gradient ultracentrifugation procedure, dual ion-exchange procedures enhanced removal of the empty capsids to realize highly-purified vector production at hand. As an application for the AAV-mediated protein supplementation therapy, therapeutic effects of anti-inflammatory treatment on the mice stroke model were successfully demonstrated. Furthermore, vector-producing mesenchymal stem cells to target glioma were developed to target seat of disease with improved anti-tumor effects in vivo.
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Report
(5 results)
Research Products
(73 results)
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[Journal Article] Systemic delivery of IL-10 by an AAV vector prevents vascular remodeling and end-organ damage in stroke-prone spontaneously hypertensive rat.2009
Author(s)
Nomoto T., Okada T., Shimazaki K., Yoshioka T., Nonaka-Sarukawa M., Ito T., Takeuchi K., Katsura K.I., Mizukami H., Kume A., Ookawara S., Ikeda U., Katayama Y., Ozawa K.
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Journal Title
Gene Ther 16
Pages: 383-91
Related Report
Peer Reviewed
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[Journal Article] Scalable purification of AAV1 and AAV8 vectors using dual ion-exchange adsorptive membranes2009
Author(s)
Okada, T., Nonaka-Sarukawa, M., Uchibori, R., Kinoshita, K., Hayashita-Kinoh, H., Nitahara-Kasahara, Y., Takeda, S., Ozawa, K.
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Journal Title
Hum Gene Ther 20
Pages: 1013-1021
Related Report
Peer Reviewed
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[Journal Article] Systemic delivery of IL-10 by an AAV vector prevents vascular remodeling and end-organ damage in stroke-prone spontaneously hypertensive rat2009
Author(s)
Nomoto, T., Okada, T., Shimazaki, K., Yoshioka, T., Nonaka-Sarukawa, M., Ito, T., Takeuchi, K., Katsura, K. I., Mizukami, H., Kume, A., Ookawara, S., Ikeda, U., Katayama, Y., Ozawa, K.
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Journal Title
Gene.Ther 16
Pages: 383-91
Related Report
Peer Reviewed
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[Journal Article] Interleukin-10 Expression Mediated by an Adeno-Associated Virus Vector Prevents Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats.2007
Author(s)
Ito T., Okada T., Miyashita H., Nomoto T., Nonaka-Sarukawa M., Uchibori R., Maeda Y., Urabe M., Mizukami H., Kume A., Takahashi M., Ikeda U., Shimada K., Ozawa K.
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Journal Title
Circ Res 101
Pages: 734-741
Related Report
Peer Reviewed
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[Journal Article] Interleukin-10 Expression Mediated by an Adeno-Associated Virus Vector Prevents Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats2007
Author(s)
Ito, T., Okada, T., Miyashita, H., Nomoto, T., Nonaka-Sarukawa, M., Uchibori, R., Maeda, Y., Urabe, M., Mizukami, H., Kume, A., Takahashi, M., Ikeda, U., Shimada, K., Ozawa, K.
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Journal Title
Circ Res 101
Pages: 734-741
Related Report
Peer Reviewed
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