The development of noninvasive and economical method to evaluate the bone quality using the measurement of serum levels of osteocyte-specific-proteins
Project/Area Number |
19390393
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Osaka University |
Principal Investigator |
HASHIMOTO Jun Osaka University, 医学系研究科, 准教授 (40237938)
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Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Hideki 大阪大学, 医学系研究科, 教授 (60191558)
MORIYAMA Toshiki 大阪大学, 保健センター, 教授 (30283815)
MYOUI Akira 大阪大学, 医学部附属病院, 准教授 (10263261)
NAKAMURA Norimasa 大阪大学, 臨床医工学融合研究教育センター, 招聘教授 (50273719)
|
Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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Keywords | MEPE / 骨細胞 / 骨質 / 骨強度 / 酸素分圧 / 椎体骨折 / 血漿中濃度 / 動脈硬化 / 健康寿命 / ELISA / 骨強度評価 / 血中濃度 |
Research Abstract |
It is revealed by our preliminary analysis that serum level of matrix extracellular phosphoglycoprotein (MEPE) that is one of osteocyte-specific-proteins is correlated with the multiplicity of osteoporotic vertebral fracture. However, the amino sequence analysis of MEPE derived fragment present in serum using monoclonal antibodies against MEPE revealed that mAb also bind with the unknown peptide derived from some protein other than MEPE. Identification of this foreign peptide is unfortunately too difficult due to its small amount and the cross reactivity itself in mAb, so clinical application of ELISA using mAb against MEPE has not been established. However, our in vitro study confirmed that the possible importance of the measurement of osteocyte-specific protein and showed the next target protein for development of clinically applicable plasma concentration measurement.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Sperum adiponectin concentrations correlate with severity of rheumatoid arthritis evaluated by extent of joint destruction.2009
Author(s)
Ebina K, Fukuhara A, Ando W, Hirao M, Koga T, Oshima T, Matsuda M, Maeda K, Nakamura T, Ochi T, Shimomura I, Yoshikawa H, Hashimoto J
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Journal Title
Clinical Rheumatology 28
Pages: 445-51
Related Report
Peer Reviewed
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[Journal Article] Serum adiponectin concentrations correlate with severity ofrheumatoid arthritis evaluated by extent of joint des truetion2009
Author(s)
Ebina K, Fukuhara A, Ando W, Hirao M, Koga T, Oshima T, Matsuda M, Maeda K, Nakamura T, Ochi T, Shimomura I, Yoshikawa H, Hashimoto J
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Journal Title
Clinical Rheumatology (Epub)
Related Report
Peer Reviewed
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[Journal Article] Adenovirus-mediated gene transfer of adiponectin reduces theseverity of co11agen-induced arthritis in mice.2009
Author(s)
Ebina K, Oshima K, Matsuda M, Fukuhara A, Maeda K, Kihara S, Hashimoto J, Ochi T, Banda NK, Yoshikawa H, Shimomura I.
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Journal Title
Biochem Biophys Res Commun. 378
Pages: 186-191
Related Report
Peer Reviewed
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[Journal Article] In Vitro Generation of a Scaffold-Free Tissue-Engineered Construct (TEC) Derived from Human Synovial Mesenchymal Stem Cells: Biological and Mechanical Properties, and Further Chondrogenic Potential.2008
Author(s)
Ando W, Tateishi K, Katakai D, Hart DA, Higuchi C, Nakata K, Hashimoto J, Fujie H, Shino K, Yoshikawa H, Nakamuara N
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Journal Title
Tissue Eng Part A 14(12)
Pages: 2041-9
Related Report
Peer Reviewed
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[Journal Article] Characteristics of fracture and related factors in patientswith rheumatoid arthritis2008
Author(s)
Kampei A, Hashimoto J, Koyanagi J, Ono T, Hashimoto H, Tsumaki N, Tomita T, Sugamoto K, Nishimoto N, Ochi T, Yoshikawa H
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Journal Title
Modern Rheumatology 18
Pages: 180-186
Related Report
Peer Reviewed
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[Journal Article] In Vitro Generation of a Scaffold-Free Tissue-Engineered Construct(TEC)Derived from Human Synovia1 Mesenchyma1 stem Ce11s : Biological and Mechanical Properties, and Further Chondrogenic Potential.2008
Author(s)
Ando W, Tateishi K, Katakai D, Hart DA, Higuchi C, Nakata K, Hashimoto J, Fujie H, Shino K, Yoshikawa H. Nakamura N.
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Journal Title
Tissue Eng Part A 14
Pages: 2041-2049
Related Report
Peer Reviewed
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