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A proposal of new concept of tumorgenesis in human testicular cancer by abnormal de-methylation of interspersed elements

Research Project

Project/Area Number 19390413
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionShiga University of Medical Science

Principal Investigator

OKAMOTO Keisei  Shiga University of Medical Science, 医学部, 講師 (50303780)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Takahiro (OKADA Yusaku)  滋賀医科大学, 医学部, 非常勤講師 (90346023)
JOHNIN Kazuyoshi  滋賀医科大学, 医学部, 助教 (90324590)
岡田 裕作  滋賀医科大学, 医学部, 教授 (20127062)
牛田 博  滋賀医科大学, 医学部, 助教 (80418756)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords精巣腫瘍 / 散在性反復配列 / 脱メチル化 / メチルトランスフェラーゼ / エピジェネティックス / メチル化 / 発癌モデル
Research Abstract

Our group has continuosly studied on epigenetics of testicular germ cell tumors (TGCTs) and shown that DNA of TGCTs has distinctive demethylated DNA profiles that differ from those of somatic tissue-derived cancers or somatic tissues (Int J Androl 2007). We have already described the potential clinical utility of this unique methylation phenotype in TGCTs with regard to developing a novel tumour marker. In this study, we have tried to establish a new tumorigenesis model based on abnormal demathylation of DNA repetitive elements in TGCTs. Through this study, we have obtained evidence that DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma (Clin Cancer Res 2010). Also, we have shown that unique methylation patterns of DNA repetitive elements are present in TGCTs. These methylation patterns most likely reflect the origin of these cells. Further elucidation of the mechanisms behind the maintenance of DNA hypomethylation in TGCTs is necessary in order to clarify the basic biology of this unique neoplasm.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (18 results)

All 2010 2009 2007

All Journal Article (11 results) (of which Peer Reviewed: 8 results) Presentation (7 results)

  • [Journal Article] A rare case of multiple schwannomas presenting with scrotal mass : a probable case of schwannomatosis.2010

    • Author(s)
      Ikari R, 岡本圭生, Yoshida T, Johnin K, Okabe H, Okada Y.
    • Journal Title

      Int J Urol 17

      Pages: 734-736

    • NAID

      10027259708

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Primary synovial sarcoma arising from a crossed ectopic kidney with fusion2010

    • Author(s)
      Kageyama S, Tsuru T, 岡本圭生, Narita M, Okada Y.
    • Journal Title

      Int J Urol 17

      Pages: 96-98

    • NAID

      10027256655

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma2010

    • Author(s)
      Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, 岡本圭生
    • Journal Title

      Clin Cancer Res 16

      Pages: 2751-9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 発生起源から考える精巣腫瘍の生物学的特性2010

    • Author(s)
      岡本圭生
    • Journal Title

      日本臨床 68巻増刊号4

      Pages: 453-456

    • Related Report
      2009 Final Research Report
  • [Journal Article] DNMT3L is a novel marker and essential for the growth of human embryonal carcinoma2010

    • Author(s)
      Minami K, Okamoto K, et al.
    • Journal Title

      Clin Cancer Res (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primary synovial sarcoma arising from a ectopic kidney with fusion2010

    • Author(s)
      Kageyama S, Okamoto K, et al.
    • Journal Title

      Int J Urol 17

      Pages: 96-98

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 発生起源からみた精巣腫瘍の分子生物学的特性と新たなバイオマーカーの探索2009

    • Author(s)
      岡本圭生
    • Journal Title

      Urology View 7

      Pages: 116-121

    • Related Report
      2009 Final Research Report
  • [Journal Article] 発生起源からみた精巣腫瘍の分子生物学的特性2009

    • Author(s)
      岡本圭生
    • Journal Title

      日本臨床 腎・泌尿器癌 増刊号

      Pages: 453-456

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Epigenetic profile of testicular germ cell tumours2007

    • Author(s)
      岡本圭生, Kawakami T
    • Journal Title

      Int J Androl 30

      Pages: 385-392

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic profile of testicular germ cell tumours.2007

    • Author(s)
      Okamoto K and Kawalcami T.
    • Journal Title

      Int J Androl 30

      Pages: 385-92

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bilateral sinlgle ectopic ureters with hypoplastic bladder:How should we treat these challenging entities2007

    • Author(s)
      Johnin K, Okada Y, et. al.
    • Journal Title

      J Pediatr Urol 3

      Pages: 243-246

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mutational analysis of P53 gene in cancer cells presenting loss of the inactive X chromosome.2010

    • Author(s)
      南かほり, Takahiro Kawakami, 茶野徳宏, 岡部英俊, 岡本圭生
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会
    • Related Report
      2009 Final Research Report
  • [Presentation] 散在性反復配列のエピジェネティックス異常が精巣腫瘍発生に果たす意義にっいて2010

    • Author(s)
      牛田博、南かほり、茶野徳宏、岡部英俊、岡田裕作、岡本圭生.
    • Organizer
      第98回日本泌尿器科学会総会
    • Related Report
      2009 Final Research Report
  • [Presentation] DNMT3L is a novel marker and essential for the growth of human embryonal carcinoma.2010

    • Author(s)
      Hiroshi Ushida, 南かほり, 茶野徳宏, Takahiro Kawakami, 岡部英俊, 岡田裕作, 岡本圭生
    • Organizer
      The 27th Japan-Korea Urological Congress
    • Related Report
      2009 Final Research Report
  • [Presentation] DNMT3L is a novel marker for human embryonal carcinoma of the testis2009

    • Author(s)
      南かほり, 岡本圭生
    • Organizer
      日本癌学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of fetal specific methyltransferase like protein DNMT3L in testicular germ cell tumors.2009

    • Author(s)
      南かほり, 茶野徳宏, 岡部英俊, 岡本圭生
    • Organizer
      第68回日本癌学会総会
    • Related Report
      2009 Final Research Report
  • [Presentation] 精巣腫瘍におけるDNMT3lの解析2009

    • Author(s)
      牛田博、南かほり、茶野徳宏、岡部英俊、岡田裕作、岡本圭生.
    • Organizer
      第97回日本泌尿器科学会総会
    • Related Report
      2009 Final Research Report
  • [Presentation] DNMT3L is a novel marker for human embryonal carcinoma of the testis2009

    • Author(s)
      南かほり, 茶野徳宏, Takahiro Kawakami, 牛田博, Ryoji Kushima, 岡部英俊, 岡田裕作, 岡本圭生
    • Organizer
      第69回日本癌学会
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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