| Project/Area Number |
19390413
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
OKAMOTO Keisei Shiga University of Medical Science, 医学部, 講師 (50303780)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Takahiro (OKADA Yusaku) 滋賀医科大学, 医学部, 非常勤講師 (90346023)
JOHNIN Kazuyoshi 滋賀医科大学, 医学部, 助教 (90324590)
岡田 裕作 滋賀医科大学, 医学部, 教授 (20127062)
牛田 博 滋賀医科大学, 医学部, 助教 (80418756)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 精巣腫瘍 / 散在性反復配列 / 脱メチル化 / メチルトランスフェラーゼ / エピジェネティックス / メチル化 / 発癌モデル |
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| Research Abstract |
Our group has continuosly studied on epigenetics of testicular germ cell tumors (TGCTs) and shown that DNA of TGCTs has distinctive demethylated DNA profiles that differ from those of somatic tissue-derived cancers or somatic tissues (Int J Androl 2007). We have already described the potential clinical utility of this unique methylation phenotype in TGCTs with regard to developing a novel tumour marker. In this study, we have tried to establish a new tumorigenesis model based on abnormal demathylation of DNA repetitive elements in TGCTs. Through this study, we have obtained evidence that DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma (Clin Cancer Res 2010). Also, we have shown that unique methylation patterns of DNA repetitive elements are present in TGCTs. These methylation patterns most likely reflect the origin of these cells. Further elucidation of the mechanisms behind the maintenance of DNA hypomethylation in TGCTs is necessary in order to clarify the basic biology of this unique neoplasm.
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