Clinical development of early detection method for renal cell carcinoma with serum cancer markers and methylated DNA-fragment cancer markers

• Project/Area Number: 19390417
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: SHUIN Taro Kochi University, 教育研究部医療学系, 教授 (80179019); 執印 太郎 (2008) Kochi University, 教育研究部医療学系, 教授 (70128601)
• Co-Investigator(Kenkyū-buntansha): 蘆田 真吾 高知大学, 医学部附属病院, 助教 (80380327) ; 井上 啓史 高知大学, 医学部, 准教授 (00294827)
• Co-Investigator(Renkei-kenkyūsha): ASHIDA Shingo 高知大学, 教育研究部医療学系, 助教 (80380327) ; INOUE Keiji 高知大学, 教育研究部医療学系, 准教授 (00294827)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 腎細胞癌 / 腫瘍マーカー / HIG2 / Hoxb13 / メチル化 / HOXB13 / メチル化DNA

Research Abstract

ヒト腎の問題点は診断マーカーが存在しないため画像以外には早期診断が難しいこと、また転移の早期発見が難しく転移癌への有効な治療法が存在しないことである。そのためヒトDNAメチル化マーカーと血清マーカーの開発を検討した。その結果血清マーカーHIG2, TMEM22などの有用性を検討し明らかにした。またmethylated DAN marker HOXb13などを中心にメチル化DNAマーカーについても蛋白の発現誘導実験を行い、新しいマーカーの存在を検討した。

Research Products

• [Journal Article] Evidence-based clinical practice guidelines for renal cel carcinoma(Summary-JUA 2007 Edition) (2009)
  • Author(s): Fujioka T, Obara W, Miki Hara I, Hirao Y, Kakehi Y, Kanayama HO, Naito S, Nonomura N, Ogawa Y, Ozono S, Shimazui T, Shinohara N, Shuin T, Takahashi S, Tomita Y, Eto M, Inoue K, Kato T, Kinouchi T, Konda R, Mugiya S, Nagamori S, Nakazawa H, Nishitani MA, Oikawa T, Sugimoto M, Takahashi M, Mizutani Y, Obara W.
  • Journal Title: Int J Urol. 16(4) Pages: 339-339
  • Peer Reviewed
• [Journal Article] Involvement of TMEM22 overexpressionin the growth of renal cell carcinomacells (2009)
  • Author(s): Dobashi S, Katagiri T, Hirota E, Ashida S, Daigo Y, Shuin T, Fujioka T, Miki T, Nakamura Y
  • Journal Title: OncolRep. 21(2) Pages: 305-312
  • Peer Reviewed
• [Journal Article] Involvement of TMEM22 overexpression in the growth of renal cell carcinoma cells. (2009)
  • Author(s): Dobashi S, Shuin T, et al.
  • Journal Title: Oncology Report 21 Pages: 305-312
  • Peer Reviewed
• [Journal Article] Chromophobe renal cell carcinoma: useful diagnostic application imprinttology and fluorescence in situ hybridization of chromosomes 10 and 21 in two cases of typical and eosinophilic variants (2008)
  • Author(s): Kuroda N, Katto K, Yamaguchi T, KaT, Imamura Y, Hes O, Michal M, Shuin T, Lee GH.
  • Journal Title: MedMolMorphol 41(4) Pages: 227-232
  • Peer Reviewed
• [Journal Article] Acute inactivation of the VHL gene contributes to protective effects of ischemic preconditioning in the mouse kidney (2008)
  • Author(s): Iguchi M, Kakinuma Y, Kurabayashi A, Sato T, Shuin T, Hong SB, Schmidt LS, Furihata M.
  • Journal Title: Nephron Exp Nephrol. 110(3)
  • Peer Reviewed
• [Journal Article] Sarcomatoid acquired cystic disease-associated renal cell carcinoma (2008)
  • Author(s): Kuroda N, Tamura M, Taguchi T, Tominaga A, Hes O, Michal M, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Shuin T, Lee GH.
  • Journal Title: HistolHistopathol 23(11) Pages: 1327-1331
  • Peer Reviewed
• [Journal Article] Promoter methylation of the bone morphogenetic protein-6 gene in association with adult T-cll leukemia (2008)
  • Author(s): Taniguchi A, Nemoto Y, Yokoyama AKotani N, Imai S, Shuin T, Daibata M.
  • Journal Title: Int J Cancer 123(8) Pages: 1824-131
  • Peer Reviewed
• [Journal Article] Prospective study of combined treatment with interferon-alpha and active vitamin D3 for Japanese patients with metastatic renal cellcarcinoma (2008)
  • Author(s): Obara W, Mizutani Y, Oyama C, Akaza HIshii N, Kohri K, Namiki M, Okuyama A, Shima H, Yokoyama M, Shuin T, Miki T, Watanabe Y Fujioka T.
  • Journal Title: Int J Urol. 15(9) Pages: 794-799
  • Peer Reviewed
• [Journal Article] Sarcomatoid acquired cystic disease-associated renal cell carcinoma. (2008)
  • Author(s): Kuroda N, Shuin T, et al.
  • Journal Title: Histology Histopathology 23 Pages: 1327-1331
  • Peer Reviewed
• [Journal Article] KTernary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy technique (2007)
  • Author(s): Kinoshita K, Coryo K, Takada M, Tomokuni Y, Aso T, Okuda H, Shuin T, Fukumura H, Sogawa
  • Journal Title: FEBS J. 274(21) Pages: 5567-75
  • Peer Reviewed
• [Journal Article] WT1(Wilms' tumor 1)peptide immunotherapy for renal cell carcinoma (2007)
  • Author(s): Iiyama T, Udaka K, Takeda S, Takeuchi T, Adachi YC, Ohtsuki Y, Tsuboi A, Nakatsuka S, Elisseeva OA, Oji Y, Kawakami M, Nakajima H, Nishida S, Shirakata T, Oka Y, Shuin T, Sugiyama H.
  • Journal Title: Mcrobi Imnol. 51(5) Pages: 519-30
  • Peer Reviewed
• [Journal Article] Promoter hypermethylation of the bone morphogenetic protein-6 gene in malignant lymphoma (2007)
  • Author(s): Daibata M, Nemoto Y, BandobashiKotani N, Kuroda M, Tsuchiya M, Okuda H, Takakuwa T, Imai S, Shuin T, Taguchi H.
  • Journal Title: Clin Cancer Res. 13(12) Pages: 3528-3535
  • Peer Reviewed
• [Journal Article] Modification in the von Hippel-Lindau protein is involved in the progression of experimentally induced rat glomrulonephritis (2007)
  • Author(s): Kudo Y, Kakinuma Y, Iguchi M, Sato TSugiura T, Furihata M, Shuin T.
  • Journal Title: ephron Exp Nephrol 106(3)
  • Peer Reviewed
• [Journal Article] Renal leiomyoma : an immunohistochemical, ultrastructural and comparative genomic hybridizion study Histol Histopathol (2007)
  • Author(s): Kuroda N, Inoue Y, Taguchi T, Tominaga A, Hes O, Michal M, Hayashi Y, Hiroi M, Shuin T, Lee GH.
  • Journal Title: 22(8) Pages: 883-8
  • Peer Reviewed
• [Journal Article] Blockade of the vascular endothelial growth factor-receptor 2 pathway inhibits the growth of human renal cell carcinoma, RBM1-IT4, in the kidney but not in the bone of nude mice (2007)
  • Author(s): Karashima T, Inoue K, Fukata S, Iiyama T, Kurabayashi A, Kawada C, Shuin T.
  • Journal Title: Int J Oncol 30(4) Pages: 937-45
  • Peer Reviewed
• [Presentation] (2007)
  • Author(s): 奥田平和
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-05
• [Presentation] 腎細胞癌におけるHOXB13癌抑制遺伝子の転写標的の同定 (2007)
  • Author(s): 奥田平和
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05