Study for mechanism of gonadotropin action on follicular and oocyte maturation

• Project/Area Number: 19390425
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Gunma University
• Principal Investigator: MINEGISHI Takashi Gunma University, 大学院・医学系研究科, 教授 (00209842)
• Co-Investigator(Kenkyū-buntansha): ITOH Masahiro 群馬大学, 医学部, 准教授 (20282402) ; SHINOZAKI Hiromitsu 群馬大学, 医学部, 教授 (30334139) ; IGARASHI Shigeo 群馬大学, 医学部, 講師 (60343084)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: LH-R / E_2 / FSH-R / β-グリカン / TGF-β / E2

Research Abstract

GRP78は小胞体において蛋白が高次構造を獲得するのに関与している分子シャペロンである。LHレセプター(LHR)を恒常的に発現している細胞にGRP78を発現させるとLHRとGRP78が結合し、更にGRP78発現量依存的にLHR発現が増加することを確認した。ヒト、ラットともhCG添加に伴いGRP78 promoter活性の上昇がみられ、またhCGにエストロゲンを加えることで若干ではあるが更なるpromoter活性上昇がみられた。LH/hCGによりGRP78 promoter活性が変化し、LHR発現調節にGRP78が関与することを強く示唆する結果が得られた。

Research Products

• [Journal Article] Expression and Cyclic Change of Betaglycan in the Rat Oviduct (2009)
  • Author(s): Kubota K, Omori Y, Ikeda S, Minegishi T
  • Journal Title: J Reprod Dev (印刷中)
  • Peer Reviewed
• [Journal Article] Expression and Cyclic Change of Betaglycan in the Rat Oviduct. (2009)
  • Author(s): Kubota, Kazuko
  • Journal Title: Journal of Reproduction and Develonment (印刷中)
  • Peer Reviewed
• [Journal Article] Primary hypothyroidism presenting as multiple ovarian cysts in an adult woman : a case report (2008)
  • Author(s): Kubota K, Itho M, Kishi H, Igarashi S, Minegishi T
  • Journal Title: Gynecol Endocrinol 24(10) Pages: 586-589
  • Peer Reviewed
• [Journal Article] Auersperg N, Leung PC, HOX cofactors expression and regulation in the human ovary (2008)
  • Author(s): Ota T, Asahina H, Park SH, Huang Q, Minegishi T
  • Journal Title: Reprod Biol Endocrinol 6 Pages: 49-49
  • Peer Reviewed
• [Journal Article] Compartment syndrome with thrombosis of common iliac artery after gynecologic surgery (2008)
  • Author(s): Nakamura K, Aoki H, Hirakawa T, Murata T, Kanuma T, Minegishi T
  • Journal Title: Obstet Gynecol 112(2Pt2) Pages: 486-488
  • Peer Reviewed
• [Journal Article] Expression of an activated mammalian target of rapamycin in adenocarcinoma of the cervix : A potential biomarker and molecular target therapy (2008)
  • Author(s): Faried LS, Faried A, Kanuma T, Aoki H, Sano T, Nakazato T, Tamura T, Kuwano H, Minegishi T
  • Journal Title: Mol Carcinog 47(6) Pages: 446-457
  • Peer Reviewed
• [Journal Article] Regulation of human luteinizing hormone receptor in the ovary (2008)
  • Author(s): Minegishi T, Nakamura K, Yamashita S, Ikeda S, Kogure K
  • Journal Title: Reprod Med Biol 7(1) Pages: 11-16
  • NAID: 10021246872
  • Peer Reviewed
• [Journal Article] Effect of Estrogen on the Expression of Luteinizing Hormone-Human Chorionic Gonadotropin Receptor Messenger Ribonucleic Acid in Cultured Rat Granulosa Cells (2008)
  • Author(s): Ikeda S, Nakamura K, Kogure K, Omori Y, Yamashita S, Kubota K, Mizutani T, Miyamoto K, Minegishi T
  • Journal Title: Endocrinology 149(4) Pages: 1524-1533
  • Peer Reviewed
• [Journal Article] Expression of an activated mammalian target of rapamycin in adenocarci-noma of the cervix: A potential biomarker and molecular target therapy. (2008)
  • Author(s): Faried LS
  • Journal Title: Molecular Carcinogenesis 47 Pages: 446-457
  • Peer Reviewed
• [Journal Article] Compartment syndrome with thrombosis of common iliac artery after gynecologic surgery. (2008)
  • Author(s): Nakamura, Kazuto
  • Journal Title: Obstetrics & Gynecology 112 Pages: 486-488
  • Peer Reviewed
• [Journal Article] HOX cofactors expression and regulation in the human ovary. (2008)
  • Author(s): Ota, Takayo
  • Journal Title: Reproductive biology and endocrinology 6 Pages: 49-49
  • Peer Reviewed
• [Journal Article] Primary hypothyroidism presenting as multiple ovarian cysts in an adult woman: a case report. (2008)
  • Author(s): Kubota, Kazuko
  • Journal Title: Gynecological Endocrinology 24 Pages: 586-589
  • Peer Reviewed
• [Journal Article] Effect of on the Expression of Luteinizing Hormone-Human Cthorionic Gonadotropin Reoeptor Messenger Ribonucleic Acid in Cultured Rat Granulosa Cells (2008)
  • Author(s): Sadatomo Ikeda
  • Journal Title: Endocrinology (掲載決定)
  • Peer Reviewed
• [Journal Article] Regulation of human luteinizing hormone receptor in the ovary (2008)
  • Author(s): Takashi Minegishi
  • Journal Title: Reproductive Medicine and Biology 7 Pages: 11-16
  • NAID: 10021246872
  • Peer Reviewed
• [Journal Article] 日本産科婦人科学会研修コーナーC.産婦人科検査法1.内分泌・不妊検査法5)血中ホルモン測定6)ホルモン負荷試験 (2007)
  • Author(s): 峯岸敬
  • Journal Title: 日本産科婦人科学会雑誌 59(4) Pages: 43-43
• [Journal Article] 日本産科婦人科学会研修コーナーC.産婦人科検査法 1.内分泌・不妊検査法 5)血中ホルモン測定 6)ホルモン負荷試験 (2007)
  • Author(s): 峯岸 敬
  • Journal Title: 日本産科婦人科学会雑誌 59 Pages: 39-43
• [Presentation] The effect of 78?kilodalton glucose-regulated protein on the expression of rat luteinizing hormone receptor. (2008)
  • Author(s): Nakamura, Kazuto
  • Organizer: 13th International Congress of Endocrinologl (ICE)
  • Place of Presentation: ブラジル
  • Year and Date: 2008-11-10
• [Presentation] The effect of 78-kilodalton glucose-regulated protein on the expression of rat luteinizing hormone receptor (2008)
  • Author(s): Nakamura K, Kogure K, Kubota K, Ikeda S, Minegishi T
  • Organizer: 13^<th> International Congress of Endocrinology
  • Place of Presentation: リオ・デ・ジャネイロ
  • Year and Date: 2008-11-01
• [Presentation] ラット卵巣におけるLHRおよびGRP78発現量変化についての検討 (2008)
  • Author(s): 小暮佳代子、中村和人、久保田和子、池田禎智、峯岸敬
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: 神奈川
  • Year and Date: 2008-04-14
• [Presentation] ラット卵巣におけるLHRおよびGRP78発現量変化についての検討 (2008)
  • Author(s): 小暮佳代子
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: 神奈川県
  • Year and Date: 2008-04-14
• [Presentation] Progress Report British Columbia-Gunma (2007)
  • Author(s): Minegishi T
  • Organizer: 4th Canada-Japan Bilateral Workshop on Human Reproduction Biology(Canada-Japan Bilateral Symposium on Womens Reproductive Health)
  • Place of Presentation: 青森
  • Year and Date: 2007-08-01
• [Presentation] Progress Report British Columbia-Gunma (2007)
  • Author(s): 峯岸 敬
  • Organizer: 4th Canada-Japan Bilateral Workshop on Huma Reproduction Biology
  • Place of Presentation: 弘前市
  • Year and Date: 2007-08-01
• [Presentation] 不妊症患者の抗リン脂質抗体出現頻度 (2007)
  • Author(s): 伊藤理廣、五十嵐茂雄、岸裕司、田村友宏、池田禎智、峯岸敬
  • Organizer: 第59回日本産科婦人科学会総会・学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2007-04-16
• [Presentation] 不妊症患者の抗リン脂質抗体出現頻度 (2007)
  • Author(s): 伊藤 理廣
  • Organizer: 第59回日本産科婦人科学会総会・学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2007-04-16
• [Book] Annual Review糖尿病・代謝・内分泌2009 (2009)
  • Author(s): 峯岸敬
  • Publisher: 中外医学社
• [Book] Anllual Review 糖尿病・代謝・内分泌 2009 (2009)
  • Author(s): 峯岸敬
  • Publisher: 中外医学社
• [Remarks]
  • URL: http://obgyn.dept.med.gunma-u.ac.jp/
• [Remarks]
  • URL: http://obgyn.dept.med.gunma-u.jp/