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Functional analysis of fusogenic factors related to infertility and its clinical application

Research Project

Project/Area Number 19390431
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNational Research Institute for Child Health and Development

Principal Investigator

MIYADO Kenji  National Research Institute for Child Health and Development, 生殖・細胞医療研究部, 室長 (60324844)

Co-Investigator(Kenkyū-buntansha) AKUTSU Hidenori  独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 室長 (50347225)
宮本 潔子  国立成育医療センター(研究所), 生殖・細胞医療研究部, 共同研究員 (20415590)
斉藤 英和 (斎藤 英和)  国立成育医療センター(研究所), 生殖・細胞医療研究部, 共同研究員 (90125766)
高橋 祐司  国立成育医療センター(研究所), 生殖・細胞医療研究部, 共同研究員 (00392499)
宮戸 真美  国立成育医療センター(研究所), 生殖・細胞医療研究部, 共同研究員 (00386252)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords生殖医学 / 受精 / 膜融合 / CD9 / エキソソーム / チューブリン / テトラスパニン / テトラスバニン
Research Abstract

Membrane fusion is an essential step in the encounter of two nuclei from sperm and egg in fertilization. Here, we showed that sperm-egg fusion is mediated by vesicles containing CD9 that are released from the egg and interact with sperm.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (90 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (48 results) (of which Peer Reviewed: 35 results) Presentation (27 results) Book (11 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage.2011

    • Author(s)
      Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N.
    • Journal Title

      BMC Dev Biol.

      Pages: 11-22

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipid Rafts : Keys to SpermMaturation, Fertilization, and Early Embryo genesis.2011

    • Author(s)
      Kawano N, Yoshida K, Miyado K, Yoshida M.
    • Journal Title

      Journal of Lipids

      Volume: 2011

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of CD9 and CD9-containing exosomes in sperm-egg membrane fusion.2010

    • Author(s)
      Kawano N., Yoshida K., Harada Y., Onami N., Takezawa Y., Miyado K.
    • Journal Title

      J Mamm Ova Res. 27(4)

      Pages: 191-197

    • NAID

      10027595414

    • Related Report
      2010 Final Research Report
  • [Journal Article] Age-associated changes in the subcellular localization of phosphorylated p38 MAPK in human granulosa cells.2010

    • Author(s)
      Ito M, Miyado K, Nakagawa K, Muraki M, Imai M, Yamakawa N, Qin J, Hosoi Y, Saito H, Takahashi Y.
    • Journal Title

      Mol Hum Reprod. 16(12)

      Pages: 928-37

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the Mutant Sperm are infertile in vitro.2010

    • Author(s)
      Kawano N., Kang W., Yamashita M., Koga Y., Yamazaki T., Hata T., Miyado K., Baba T.
    • Journal Title

      Biol Reprod. 83(3)

      Pages: 359-69

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Xenografted human amniotic membrane-derived mesenchymal stem cells were immunologically tolerated and transdifferentiated into cardiomyocytes.2010

    • Author(s)
      Tsuji H, Miyoshi S, Ikegami Y, Hida N, Asada H, Togashi I, Suzuki J, Satake M, Nakamizo H, Tanaka M, Mori T, Segawa K, Nishiyama N, Inoue J, Makino H, Miyado K, Ogawa S, Yoshimura Y, Umezawa A.
    • Journal Title

      Circulation Res 106(10)

      Pages: 1613-23

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tetraspanin family protein CD9 in the mouse sperm : unique localization, appearance, behavior and fate during fertilization.2010

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toshimori K.
    • Journal Title

      Cell Tissue Res. 340(3)

      Pages: 583-94

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum-independent cardiomyogenic transdifferentiation in human endometrium-derived mesenchymal cells.2010

    • Author(s)
      Ikegami Y, Miyoshi S, Nishiyama N, Hida N, Okamoto K, Miyado K, Segawa K, Ogawa S, Umezawa A.
    • Journal Title

      Artif Organs 34(4)

      Pages: 280-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Age-associated changes in the subcellular localization of phosphorylated p38 MAPK in human granulosa cells.2010

    • Author(s)
      Ito M, Miyado K, Nakagawa K, Muraki M, Imai M, Yamakawa N, Qin J, Hosoi Y, Saito H, Takahashi Y.
    • Journal Title

      Mol Hum Reprod.

      Volume: 16 Pages: 928-937

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro.2010

    • Author(s)
      Kawano N, Kang W, Yamashita M, Koga Y, Yamazaki T, Hata T, Miyado K, Baba T.
    • Journal Title

      Biol Reprod.

      Volume: 83 Pages: 359-369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetraspanin family protein CD9 in the mouse sperm : unique localization, appearance, behavior and fate during fertilization.2010

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toshimori K.
    • Journal Title

      Cell Tissue Res.

      Volume: 340 Pages: 583-594

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of CD9 and CD9-containing exosomes in sperm-egg membrane fusion.2010

    • Author(s)
      Kawano N., Yoshida K., Harada Y., Onami N., Takezawa Y., Miyado K.
    • Journal Title

      J Mamm Ova Res.

      Volume: 27 Pages: 191-197

    • NAID

      10027595414

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Tetraspanin family protein CD9 in the mouse sperm : unique localization, appearance, behavior and fate during fertilization2010

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toshimori K.
    • Journal Title

      Cell Tissue Res

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Tetraspanin family protein CD9 in the mouse sperm : unique localization, appearance, behavior and fate during fertilization2010

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toshimori K.
    • Journal Title

      Cell Tissue Research (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9.2009

    • Author(s)
      Akutsu H, Miura T, Machida M, Birumachi JI, Hamada A, Yamada M, Sullivan S, Miyado K, Umezawa A.
    • Journal Title

      Differentiation 78(2-3)

      Pages: 137-42

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Equatorin : Identification and Characterization of the Epitope of the MN9 Antibody in the Mouse.2009

    • Author(s)
      Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.
    • Journal Title

      Biol Reprod 81(5)

      Pages: 889-97

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shortening of human cell life span by induction of p16ink4a through the platelet-derived growth factor receptor beta.2009

    • Author(s)
      Takahashi H, Toyoda M, Birumachi J, Horie A, Uyama T, Miyado K, Matsumoto K, Saito H, Umezawa A.
    • Journal Title

      J Cell Physiol 221(2)

      Pages: 335-42

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD92009

    • Author(s)
      Akutsu H, Miura T, Machida M, Birumachi JI, Hamada A, Yamada M, Sullivan S, Miyado K, Umezawa A.
    • Journal Title

      Differentiation 78(2-3)

      Pages: 137-42

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Equatorin : Identification and Characterization of the Epitope of the MN9 Antibody in the Mouse2009

    • Author(s)
      Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.
    • Journal Title

      Biol Reprod 81(5)

      Pages: 889-97

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD92009

    • Author(s)
      Akutsu H, Miura T, Machida M, Birumachi JI, Hamada A, Yamada M, Sullivan S, Miyado K, Umezawa A
    • Journal Title

      Differentiation 78

      Pages: 137-142

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Equatorin : Identification and Characterization of the Epitope of the MN9 Antibody in the Mouse2009

    • Author(s)
      Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.
    • Journal Title

      Biology of Reproduction 81

      Pages: 889-897

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice.2008

    • Author(s)
      Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, Miyamoto K, Akutsu H, Kondo T, Takahashi Y, Ban T, Ito C, Toshimori K, Nakamura A, Ito M, Miyado M, Mekada E, Umezawa A.
    • Journal Title

      Proc Natl Acad Sci USA(Miyado K is a corresponding author; Faculty of 1000 Biology推薦論文) 105(35)

      Pages: 12921-6

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.2008

    • Author(s)
      Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, Suzuki M, Minami S, Iwasaki T, Goya S, Kijima T, Kumagai T, Yoshida M, Osaki T, Komori T, Mekada E, Kawase I.
    • Journal Title

      J Biol Chem 283(38)

      Pages: 26089-97

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional significance of stage-specific embryonic antigens in the development of preimplantation embryos.2008

    • Author(s)
      Katagiri Y, Sato B, Miyado K, Akutsu H, Okita H, Umezawa A, Fujimoto J, Kiyokawa N.
    • Journal Title

      Trends in Glycoscience and Glycotechnology 20(113)

      Pages: 131-39

    • NAID

      10024322754

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells.2008

    • Author(s)
      Hida N, Nishiyama N, Miyoshi S, Kira S, Segawa K, Uyama T, Mori T, Miyado K, Ikegami Y, Cui C, Kiyono T, Kyo S, Shimizu T, Okano T, Sakamoto M, Ogawa S, Umezawa A.
    • Journal Title

      Stem Cells 26(7)

      Pages: 1695-704

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] What can we learn from gene expression profiling of mouse oocytes?2008

    • Author(s)
      Hamatani T, Yamada M, Akutsu H, Kuji N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A, Yoshimura Y.
    • Journal Title

      Reproduction 135(5)

      Pages: 581-92

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of CD81 in acrosome reaction of sperm in mice.2008

    • Author(s)
      Tanigawa M, Miyamoto K, Kobayashi S, Sato M, Akutsu H, Okabe M, Mekada E, Umezawa A, Miyado K.
    • Journal Title

      Mol Reprod Dev(Miyado K is a corresponding author) 75(1)

      Pages: 150-5

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility.2008

    • Author(s)
      Ito M, Muraki M, Takahashi Y, Imai M, Tsukui T, Yamakawa N, Nakagawa K, Ohgi S, Horikawa T, Iwasaki W, Iida A, Nishi Y, Yanase T, Nawata H, Miyado K, Kono T, Hosoi Y, Saito H.
    • Journal Title

      Fertil Steril 90(4)

      Pages: 1026-35

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice2008

    • Author(s)
      Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, Miyamoto K, Akutsu H, Kondo T, Takahashi Y, Ban T, Ito C, Toshimori K, Nakamura A, Ito M, Miyado M, Mekada E, Umezawa A.
    • Journal Title

      Proc Natl Acad Sci USA 105(35)

      Pages: 12921-6

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice2008

    • Author(s)
      Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, Suzuki M, Minami S, Iwasaki T, Goya S, Kijima T, Kumagai T, Yoshida M, Osaki T, Komori T, Mekada E, Kawase I.
    • Journal Title

      J Biol Chem 283(38)

      Pages: 26089-97

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells2008

    • Author(s)
      Hida N, Nishiyama N, Miyoshi S, Kira S, Segawa K, Uyama T, Mori T, Miyado K, Ikegami Y, Cui C, Kiyono T, Kyo S, Shimizu T, Okano T, Sakamoto M, Ogawa S, Umezawa A.
    • Journal Title

      Stem Cells 26(7)

      Pages: 1695-704

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Possible involvement of CD81 in acrosome reaction of sperm in mice2008

    • Author(s)
      Tanigawa M, Miyamoto K, Kobayashi S, Sato M, Akutsu H, Okabe M, Mekada E, Umezawa A, Miyado K.
    • Journal Title

      Mol Repro Dev 75

      Pages: 150-5

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility2008

    • Author(s)
      Ito M, Muraki M, Takahashi Y, Imai M, Tsukui T, Yamakawa N, Nakagawa K, Ohgi S, Horikawa T, Iwasaki W, Iida A, Nishi Y, Yanase T, Nawata H, Miyado K, Kono T, Hosoi Y, Saito H.
    • Journal Title

      Fertil Steril 90(4)

      Pages: 1026-35

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice2008

    • Author(s)
      Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, Miyamoto K, Akutsu H, Kondo T, Takahashi Y, Ban T, Ito C, Toshimori K, Nakamura A, Ito M, Miyado M, Mekada E, Umezawa A
    • Journal Title

      Proc Natl Acad Sci USA 105

      Pages: 12921-6

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice2008

    • Author(s)
      Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, Suzuki M, Minami S, Iwasaki T, Goya S, Kijima T, Kumagaia T, Yoshida M, Osaki T, Komori T, Mekada E, Kawase I
    • Journal Title

      J Biol Chem 283

      Pages: 26089-97

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] What can we learn from gene expression profiling of mouse oocytes?2008

    • Author(s)
      Hamatani T, Yamada M, Akutsu H, Kuji N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A, Yoshimura Y
    • Journal Title

      Reproduction 135

      Pages: 581-92

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of CD81 in acrosome reaction of sperm in mice2008

    • Author(s)
      Tanigawa M, Miyamoto K, Koba yashi S, Sato M, Akutsu H, Okabe M, Mekada E, Umezawa A, and Miyado K.
    • Journal Title

      Molecular Reproduction md Development 75

      Pages: 150-155

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.2007

    • Author(s)
      Sato B, Katagiri YU, Miyado K, Akutsu H, Miyagawa Y, Horiuchi Y, Nakajima H, Okita H, Umezawa A, Hata J, Fujimoto J, Toshimori K, Kiyokawa N.
    • Journal Title

      Biochem Biophys Res Commun 364(4)

      Pages: 838-43

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sonic hedgehog expression during early tooth development in Suncus murinus.2007

    • Author(s)
      Miyado M, Ogi H, Yamada G, Kitoh J, Jogahara T, Oda S, Sato I, Miyado K, Sunohara M.
    • Journal Title

      Biochem Biophys Res Commun 363(2)

      Pages: 269-75

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells.2007

    • Author(s)
      Okamoto K, Miyoshi S, Toyoda M, Hida N, Ikegami Y, Makino H, Nishiyama N, Tsuji H, Cui CH, Segawa K, Uyama T, Kami D, Miyado K, Asada H, Matsumoto K, Saito H, Yoshimura Y, Ogawa S, Aeba R, Yozu R, Umezawa A.
    • Journal Title

      Exp Cell Res 313(12)

      Pages: 2550-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro.2007

    • Author(s)
      Nishiyama N, Miyoshi S, Hida N, Uyama T, Okamoto K, Ikegami Y, Miyado K, Segawa K, Terai M, Sakamoto M, Ogawa S, Umezawa A.
    • Journal Title

      Stem Cells 25(8)

      Pages: 2017-24

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Menstrual blood-derived cells confer human dystrophin expression in the murine model of Duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation.2007

    • Author(s)
      Cui CH, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, Umezawa A.
    • Journal Title

      Mol Biol Cell 18(5)

      Pages: 1586-94

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts.2007

    • Author(s)
      Sugiki T, Uyama T, Toyoda M, Morioka H, Kume S, Miyado K, Matsumoto K, Saito H, Tsumaki N, Takahashi Y, Toyama Y, Umezawa A.
    • Journal Title

      J Cell Biochem 100(5)

      Pages: 1240-54

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos2007

    • Author(s)
      Sato B, Katagiri YU, Miyado K, Akutsu H, Miyagawa Y, Horiuchi Y, Nakajima H, Okita H, Umezawa A, Hata J, Fujimoto J, Toshimori K, Kiyokawa N.
    • Journal Title

      Biochem Biophys Res Commun 364(4)

      Pages: 838-43

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Sonic hedgehog expression during early tooth development in Suncus murinus2007

    • Author(s)
      Miyado M, Ogi H, Yamada G, Kitoh J, Jogahara T, Oda S, Sato I, Miyado K, Sunohara M.
    • Journal Title

      Biochem Biophys Res Commun 363(2)

      Pages: 269-75

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Menstrual blood-derived cells confer human dystrophin expression in the murine model of Duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation2007

    • Author(s)
      Cui CH, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, Umezawa A.
    • Journal Title

      Mol Biol Cell 18(5)

      Pages: 1586-94

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Preferential localization of SSEA-4 in interfaces between blastomeresof mouse preimplantaion embryos2007

    • Author(s)
      Sato B, Katagiri YU, Miyado K, Akutsu H, Miyagawa Y, Horiuchi Y, Nakajima H, Okita H, Umezawa A, Hata J, Fujimoto J, To Shimori K, Kiyokawa N.
    • Journal Title

      Biochem Biophys Res Comun 364

      Pages: 838-843

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipid Rafts : Keys to SpermMaturation, Fertilization, and Early Embryogenesis.

    • Author(s)
      Kawano N, Yoshida K, Miyado K, Yoshida M.
    • Journal Title

      Journal of Lipids in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 精子と卵子の融合メカニズム : 基本原理と種特異性2011

    • Author(s)
      宮戸健二
    • Organizer
      第13回麻布大学生殖発生工学セミナー
    • Place of Presentation
      麻布大学獣医学部
    • Year and Date
      2011-03-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 哺乳動物の受精に関わる分子メカニズム2010

    • Author(s)
      宮戸健二
    • Organizer
      第12回生命・環境科学交流セミナー
    • Place of Presentation
      山形大学農学部
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 哺乳動物における配偶子融合の分子認証機構.シンポジウム「植物から学ぶ受精機構:動植物共通のアロ認証機構を考える2010

    • Author(s)
      宮戸健二
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 哺乳類における配偶子融合の分子認証機構2010

    • Author(s)
      宮戸健二
    • Organizer
      日本動物学会第81回大会シンポジウム
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Signal transduction and function of sperm citrate synthase as a sperm factor in vertebrate fertilization2010

    • Author(s)
      Harada Y, Iwao Y, Miyado K
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2010-06-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ablation of beta-catenin suppresses membrane localization of E-cadherin and reduces the ability of membrane adhesion with sperm in mouse eggs2010

    • Author(s)
      Takezawa Y, Nakamura A, Sakakibara K, Kawano N, Harada Y, Yoshida K, Umezawa A, Miyado K
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2010-06-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of CD9 in sperm-egg fusion and a general role of CD9 in fusion phenomena2010

    • Author(s)
      Miyado K, Yoshida K, Harada Y, Kawano N
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2010-06-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Arrest of spermatogenesis at round spermatids in PLCZ1-deficient mice2010

    • Author(s)
      Ito M, Nagaoka K, Kuroda K, Kawano N, Yoshida K, Harada Y, Shikano T, Miyado M, Oda S, Toshimori K, Mizukami Y, Murata T, Umezawa A, Miyazaki S, Miyado K
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] The essential role of seminal vesicle secretions on the sperm fertility in the female reproductive tract2010

    • Author(s)
      Kawano N, Araki N, Yoshida K, Yoshida M, Miyado K
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Arrest of spermatogenesis at round spermatids in PLCZ1-deficient mice.2010

    • Author(s)
      Ito M, Nagaoka K, Kuroda K, Kawano N, Yoshida K, Harada, Y, Shikano T, Miyado M, Oda S, Toshimori K, Mizukami Y, Murata T, Umezawa A, Miyazaki S, Miyado K.
    • Organizer
      Symposium : Sperm formation and differentiation, 11th International Symposium on Spermatology
    • Related Report
      2010 Final Research Report
  • [Presentation] The essential role of seminal vesicle secretions on the sperm fertility in the female reproductive tract.2010

    • Author(s)
      Kawano N, Araki N, Yoshida K, Yoshida M, Miyado K.
    • Organizer
      11th International Symposium on Spermatology
    • Related Report
      2010 Final Research Report
  • [Presentation] Signal transduction and function of sperm citrate synthase as a sperm factor in vertebrate fertilization.2010

    • Author(s)
      Harada Y, Iwao Y, Miyado K.
    • Organizer
      11th International Symposium on Spermatology
    • Related Report
      2010 Final Research Report
  • [Presentation] Ablation of beta-catenin suppresses membrane localization of E-cadherin and reduces the ability of membrane adhesion with sperm in moue eggs.2010

    • Author(s)
      Takezawa Y, Nakamura A, Sakakibara K, Kawano N, Harada Y, Yoshida K, Umezawa A, Miyado K.
    • Organizer
      11th International Symposium on Spermatology
    • Related Report
      2010 Final Research Report
  • [Presentation] CD9 action in the egg and on the sperm of mice2009

    • Author(s)
      宮戸健二
    • Organizer
      第32回日本分子生物学会念会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] 受精の膜融合における卵側因子CD9とエキソソームの役割2009

    • Author(s)
      宮戸健二
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] CD9 action in the egg and on the sperm of mice2009

    • Author(s)
      Miyado K.
    • Organizer
      Gordon Research Conferences
    • Place of Presentation
      Colby-Sawyer College, New London, NH, USA.
    • Year and Date
      2009-07-20
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] CD9 action in the egg and on the sperm of mice2009

    • Author(s)
      宮戸健二
    • Organizer
      Gordon Research Conferences
    • Place of Presentation
      Colby-Sawyer College, New London, NH, USA
    • Year and Date
      2009-07-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] CD9 action in the egg and on the sperm of mice.2009

    • Author(s)
      宮戸健二
    • Organizer
      Cell-Cell Fusion Gordon Research Conference
    • Place of Presentation
      New London
    • Year and Date
      2009-07-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 細胞融合の分子メカニズム:精子と卵子の融合系からのアプローチ2009

    • Author(s)
      宮戸健二
    • Organizer
      第114回日本解剖学会総会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 受精の膜融合における卵側因子CD9とエキソソームの役割2009

    • Author(s)
      宮戸健二
    • Organizer
      シンポジウム「動植物におけるアロ認証機構」第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 膜融合を制御するテトラスパニンの役割:受精からのアプローチ2009

    • Author(s)
      河野菜摘子、宮戸健二
    • Organizer
      シンポジウム「テトラスパニンの生化学」第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 受精の膜融合における卵側因子CD9とエキソソームの役割2009

    • Author(s)
      宮戸健二
    • Organizer
      ワークショップ「動植物におけるアロ認証機構」第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 受精の膜融合におけるCD9のユニークで不可欠な挙動2009

    • Author(s)
      河野菜摘子、宮戸健二
    • Organizer
      ワークショップ「テトラスパニン研究の新展開」第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 受精の膜融合における分子メカニズム2008

    • Author(s)
      宮戸健二
    • Organizer
      第53回日本生殖医学会総会・学術講演会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2008-10-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] Tetraspanin and gamete membrane fusion2008

    • Author(s)
      宮戸健二
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      米国コネチカット州ニューヘブン
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Tetraspanin and gamete membrane fusion2008

    • Author(s)
      Miyado K.
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      New Haven, CT, USA.
    • Year and Date
      2008-06-26
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 精子と卵子の融合の分子メカニズム2007

    • Author(s)
      宮戸 健二
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Book] VI-12歯胚形成過程におけるsShh遺伝子の発現2011

    • Author(s)
      宮戸真美、尾木秀直、城ヶ原貴通、織田銑一、宮戸健二
    • Publisher
      「スンクスの生物学」学会出版センター
    • Related Report
      2010 Final Research Report
  • [Book] VI-13スンクス雄の外生殖器2011

    • Author(s)
      宮戸真美、尾木秀直、織田銑一、宮戸健二
    • Publisher
      「スンクスの生物学」学会出版センター
    • Related Report
      2010 Final Research Report
  • [Book] スンクスの生物学(VI-13スンクス雄の外生殖器)2011

    • Author(s)
      宮戸真美、尾木秀直、織田銑一、宮戸健二
    • Total Pages
      449
    • Publisher
      学会出版センター
    • Related Report
      2010 Annual Research Report
  • [Book] Cell Fusions : Regulation and Control,(Chnpter 7 : Role of CD9 in sperm-egg fusion and its general role in fusion phenomena)2011

    • Author(s)
      Kawano N., Harada Y, Yoshida K, Miyado M., Miyado K.
    • Total Pages
      436
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Book] Role of CD9 in sperm-egg fusion and its general role in fusion phenomena, Cell Fusions : Regulation and Control. Larsson, Lars-Inge (ed).2010

    • Author(s)
      Kawano N., Harada Y., Yoshida K., Miyado M., Miyado K.
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] 目で見る生殖に関連したモデル動物-受精障害モデル動物-Hormone Frontier in Gynecology 162009

    • Author(s)
      宮戸健二
    • Related Report
      2010 Final Research Report
  • [Book] 顕微鏡活用なるほどQ&A2008

    • Author(s)
      岡部勝、宮戸健二編
    • Publisher
      羊土社
    • Related Report
      2010 Final Research Report
  • [Book] 生殖における酸化ストレスの生理的役割と不妊症疾患(実験医学26)2008

    • Author(s)
      高橋祐司、宮戸健二、齋藤英和
    • Related Report
      2010 Final Research Report
  • [Book] 顕微鏡なるほどQ&A2008

    • Author(s)
      宮戸健二, 岡部勝, 編
    • Publisher
      羊土社
    • Related Report
      2009 Self-evaluation Report
  • [Book] 顕微鏡活用なるほどQ&A-意外に知らない基礎知識2008

    • Author(s)
      戸健二, 岡部勝, 編
    • Total Pages
      202
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report
  • [Book] 受精卵が作られるしくみ:精子と卵の融合機構の解明(研究報告集23)2007

    • Author(s)
      宮戸健二
    • Publisher
      財団法人 三共生命科学研究振興財団
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.ncchd.go.jp/research.php

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞の造腫瘍性試験方法及び腫瘍マーカー2010

    • Inventor(s)
      宮戸健二、梅澤明弘
    • Industrial Property Rights Holder
      ヒューマンサイエンス財団
    • Industrial Property Number
      2010-215828
    • Filing Date
      2010-09-27
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 哺乳動物卵内への細胞外物質の導入促進剤及び導入方法2010

    • Inventor(s)
      阿久津英憲、宮戸真美、宮戸健二
    • Industrial Property Rights Holder
      Japan Science and Technology Agency
    • Acquisition Date
      2010-01-29
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] Promoter for introducing extracellular substance into mammalian ovum and introduction method2010

    • Inventor(s)
      阿久津英憲, 宮戸真美, 宮戸健二
    • Industrial Property Rights Holder
      Japan Science and Technology Agency
    • Acquisition Date
      2010-11-11
    • Related Report
      2010 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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