Elucidation of association between retinal ganglion cell death and extracellular matrix in various models of optic nerve degeneration

• Project/Area Number: 19390444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kobe University
• Principal Investigator: NEGI Akira Kobe University, 大学院・医学研究科, 教授 (00189359)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Makoto 神戸大学, 大学院・医学研究科, 講師 (80273788) ; KANAMORI Akiyasu 神戸大学, 医学部附属病院, 助教 (10444572)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 緑内障 / 糖尿病網膜症 / 視神経変性 / 細胞外基質特性 / 網膜神経節細胞死 / 水チャンネル / アクアポリン / 視神経 / 網膜神経節細胞 / グリア細胞 / アポトーシス / 視神経挫滅 / 慢性高眼圧 / 糖尿病網膜証 / 軸索輸送

Research Abstract

There was a substantial difference in magnitude, spatial distribution, and time course of apoptotic death of retinal ganglion cells among three models of optic nerve degeneration induced by diabetes, optic nerve crush, and glaucoma. The changes in aquaporin expression in the retinal ganglion cells and glia in the retina and optic nerve were significantly different among these three models. Besides, the possibility was also demonstrated that the optic nerved degeneration in human glaucoma eyes reflects the scleral biomechanics.

Research Products

• [Journal Article] Elevated intraocular pressure reduces aquaporin-9 expression in rat optic nerve head and retina. (2010)
  • Author(s): N aka M, Kanamori A, Negi A, Nakamura M.
  • Journal Title: Invest Ophthalmol Vis Sci. Epub ahead print
  • Peer Reviewed
• [Journal Article] Marked cup reversal presumably associated with scleral biomechanics in a case of adult glaucoma. (2010)
  • Author(s): K akutani Y, Nakamura M, Nagai-Kusuhara A, Kanamori A, Negi A.
  • Journal Title: Arch Ophthalmol. 128 Pages: 139-141
  • Peer Reviewed
• [Journal Article] Altered expression of aquaporins 1 and 4 coincides with neurodegenerative events in retinas of spontaneously diabetic Torii rats. (2010)
  • Author(s): F ukuda M, Negi A, Nakamura M, et al.
  • Journal Title: Exp Eye Res. 90 Pages: 17-25
  • NAID: 120003323950
  • Peer Reviewed
• [Journal Article] Marked cup reversal presumably associated with scleral biomechanics in acase of adult glaucoma (2010)
  • Author(s): Yoko Kakutani
  • Journal Title: Archives of Ophthalmology 128 Pages: 139-141
  • Peer Reviewed
• [Journal Article] Altered expression of aquaporins 1 and 4 coincides with neurodegenerative events in retinas of spontaneously diabetic Torii rats (2010)
  • Author(s): Masahide Fukuda
  • Journal Title: Experimental Eye Research 90 Pages: 17-25
  • Peer Reviewed
• [Journal Article] Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo. (2009)
  • Author(s): Kanamori A, Naka M, Fukuda M, Nakamura M, Negi A.
  • Journal Title: Exp Eye Res. 88 Pages: 535-541
  • Peer Reviewed
• [Journal Article] Association of optic disc configuration and clustered visual field sensitivity in glaucomatous eyes with hemifield visual field defects. (2009)
  • Author(s): N agai-Kusuhara A, Nakamura M, Kanamori A, Negi A.
  • Journal Title: J Glaucoma 18 Pages: 62-68
  • Peer Reviewed
• [Journal Article] 早発型発達緑内障の弱視化要因 (2009)
  • Author(s): 中村誠、調廣子、溝上國義、根木昭
  • Journal Title: 日眼会誌 113 Pages: 1145-1152
  • NAID: 10026260875
  • Peer Reviewed
• [Journal Article] Serous macular detachment due to diabetic papillopathy detected using optical coherence tomography. (2009)
  • Author(s): Nakamura M, Kanamori A, Nagai-Kusuhara A, Kusuhara S, Yamada Y, Negi A.
  • Journal Title: Arch Ophthalmol 127 Pages: 105-107
  • Peer Reviewed
• [Journal Article] Comparison of mean deviation with AGIS and CIGTS scores in association with structural parameters in glaucomatous eyes. (2009)
  • Author(s): N aka M, Kanamori A, Tatsumi Y, Fujioka M, Nagai-Kusuhara A, Nakamura M, Negi A.
  • Journal Title: J Glaucoma 18 Pages: 379-384
  • Peer Reviewed
• [Journal Article] Tafluprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo. (2009)
  • Author(s): Kanamori A, Naka M, Fukuda M, Nakamura M, Negi A
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. 247 Pages: 1353-1360
  • Peer Reviewed
• [Journal Article] Association of toll-like receptor 2 gene polymorphisms with normal tension glaucoma. (2009)
  • Author(s): Naka mura J, Nakamura M, Negi A, et al
  • Journal Title: Mol Vis 15 Pages: 2905-2910
  • Peer Reviewed
• [Journal Article] Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo (2009)
  • Author(s): Akiyasu Kanamori
  • Journal Title: Experimental Eye Research 88 Pages: 535-541
  • Peer Reviewed
• [Journal Article] Regional relationship between retinal nerve fiber layer thickness and corresponding visual field sensitivity in glaucomatous eyes. (2008)
  • Author(s): K anamori A, Naka M, Nagai-Kusuhara A, Yamada Y, Nakamura M, Negi A.
  • Journal Title: Arch Ophthalmol. 126 Pages: 1500-1506
  • Peer Reviewed
• [Journal Article] Microsatellite analysis of the GLC1B locus on chromosome 2 points to NCK2 as a new candidate gene for normal tension glaucoma. (2008)
  • Author(s): Akiy ama M, Nakamura M, Negi A, et al
  • Journal Title: Br J Ophthalmol. 92 Pages: 1293-1296
  • Peer Reviewed
• [Journal Article] Nipradilol protects rat retinal ganglion cells from apoptosis induced by serum deprivation in vitro and by diabetes in vivo. (2008)
  • Author(s): Tats umi Y, Kanamori A, Nagai-Kusuhara A, Nakanishi Y, Agarwal N, Negi A, Nakamura M.
  • Journal Title: Curr Eye Res. 33 Pages: 683-692
  • Peer Reviewed
• [Journal Article] Disagreement between Heidelberg Retina Tomograph and optical coherence tomography in assessing optic nerve head configuration of eyes with band atrophy and normal eyes. (2008)
  • Author(s): Naga i-Kusuhara A, Nakamura M, Tatsumi Y, Nakanishi Y, Negi A.
  • Journal Title: Br J Ophthalmol. 92 Pages: 1382-1386
  • Peer Reviewed
• [Journal Article] Association of Toll-like receptor 4 gene polymorphisms with normal tension glaucoma. (2008)
  • Author(s): Shib uya E, Nakamura M, Negi A, et al
  • Journal Title: Invest Ophthalmol Vis Sci. 49 Pages: 4453-4457
  • Peer Reviewed
• [Journal Article] Long-term results of amniotic membrane transplantation-assisted bleb revision for leaking blebs. (2008)
  • Author(s): Naga i-Kusuhara A, Nakamura M, Fujioka M, Negi A.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. 246 Pages: 567-571
  • Peer Reviewed
• [Journal Article] Association of retinal nerve fiber layer thickness measured by confocal scanning laser ophthalmoscopy and optical coherence tomography with disc size and axial length. (2008)
  • Author(s): Naga i-Kusuhara A, Nakamura M, Fujioka M, Tatsumi Y, Negi A.
  • Journal Title: Br J Ophthalmol 92 Pages: 186-190
  • Peer Reviewed
• [Journal Article] Evaluation of optic nerve head configuration in various types of optic neuropathy with Heidelberg Retina Tomograph. (2008)
  • Author(s): Naga i-Kusuhara A, Nakamura M, Kanamori A, Nakanishi Y, Kusuhara S, Negi A.
  • Journal Title: Eye. 22 Pages: 1154-1160
  • Peer Reviewed
• [Journal Article] Regional relationship between retinal nerve fiber layer thickness and corresponding visual field sensitivity in glaucomatous eyes (2008)
  • Author(s): Akiyasu Kanamori
  • Journal Title: Archives Ophthalmology 126・11 Pages: 1500-1506
  • Peer Reviewed
• [Journal Article] Association of Toll-like receptor 4 gene polymorphisms with normal tension glaucoma. (2008)
  • Author(s): Etsuko Shibuya
  • Journal Title: Investigative Ophthalmology Visual Science 49・10 Pages: 4453-4457
  • Peer Reviewed
• [Journal Article] Association of retinal nerve fiber layer thickness measured by confocal scanning laser ophthalmoscopy and optical coherence tomography with disc size and axial length (2008)
  • Author(s): Azusa Nagai-Kusuhara
  • Journal Title: British Journal of Ophthalmology 92・2 Pages: 186-190
  • Peer Reviewed
• [Journal Article] Association of retinal nerve fiber layer thickness measured by confocal scanning laser ophthalmoscopy and optical coherence tomography with disc size and axial length. (2008)
  • Author(s): Nagai-Kusuhara Azusa
  • Journal Title: British Journal of Ophthalmology 92・2 Pages: 186-190
  • Peer Reviewed
• [Journal Article] Quantification of retinal nerve fiber layer thickness reduction associated with a relative afferent pupillary defect in asymmetric glaucoma. (2007)
  • Author(s): Tats umi Y, Nakamura M, Fujioka M, Nakanishi Y, Kusuhara A, Maeda H, Negi A.
  • Journal Title: Br J Ophthalmol 91 Pages: 633-7
  • Peer Reviewed
• [Journal Article] Comparison of Pentacam Scheimpflug camera with ultrasound pachymetry and noncontact specular microscopy in measuring central corneal thickness. (2007)
  • Author(s): Fuji oka M, Nakamura M, Tatsumi Y, Kusuhara A, Maeda H, Negi A.
  • Journal Title: Curr Eye Res 32 Pages: 89-94
  • Peer Reviewed
• [Journal Article] cAMP-responsive element binding protein mediates a cGMP/protein kinase G-dependent anti-apoptotic signal induced by nitric oxide in retinal neuro-glial progenitor cells. (2007)
  • Author(s): Naga i-Kusuhara A, Nakamura M, Mukuno H, Kanamori A, Negi A, Seigel GM.
  • Journal Title: Exp Eye Res. 84 Pages: 152-62
  • NAID: 120000945865
  • Peer Reviewed
• [Journal Article] Quantification of retinal nerve fiber layer thickness reduction associated with a relative afferent pupillary defect in asymmetric glaucoma. (2007)
  • Author(s): Yasuko Tatsumi
  • Journal Title: British Journal of Ophthalmology 91・5 Pages: 633-637
  • Peer Reviewed
• [Presentation] NTGとPOAGにおける体位変換時の眼圧変動と視野進行の関係. (2009)
  • Author(s): 溝上淳二、中村誠、根木昭
  • Organizer: 第20回日本緑内障学会.
  • Place of Presentation: 沖縄
  • Year and Date: 2009-11-14
• [Presentation] 小児緑内障の診断と治療. (2008)
  • Author(s): 根木昭
  • Organizer: 第19回日本緑内障学会.
  • Place of Presentation: 大阪.
  • Year and Date: 2008-09-14
• [Presentation] Nipradilol protects rat retinal ganglion cells from apoptosis induced by serum deprivation in vitro and by diabetes in vivo. (2008)
  • Author(s): Nakamura M, Tatsumi Y, Kanamori A, Naka M, Agarwal N, Negi A.
  • Organizer: 世界眼会議.
  • Place of Presentation: 香港
  • Year and Date: 2008-06-29
• [Presentation] Optical coherence tomographic findings of macular lesions associated with diabetic papillopathy. (2008)
  • Author(s): Nakamura M, Kanamori A, Nagai-Kusuhara A, Kusuhara S, Yamada Y, Negi A.
  • Organizer: 世界眼会議.
  • Place of Presentation: 香港
  • Year and Date: 2008-06-28
• [Presentation] Nakanishi Y, Negi A. Potential anti-apoptotic mechanisms of latanoprost and nipradilol. (2007)
  • Author(s): Nakamura M, Kanamori A, Nagai-Kusuhara A, Tatsumi Y
  • Organizer: アジアオセアニア緑内障会議.
  • Place of Presentation: タイ(バンコク)
  • Year and Date: 2007-12-03
• [Presentation] Agreement between HRT and OCT in assessing optic nerve head configuration. (2007)
  • Author(s): Nakamura M, Kanamori A, Nagai-Kusuhara A, Tatsumi Y, Nakanishi Y, Negi A.
  • Organizer: アジアオセアニア緑内障会議
  • Place of Presentation: タイ(バンコク)
  • Year and Date: 2007-12-02
• [Presentation] Structure/function relationship in glaucoma analyzed with optical coherence tomography and Heidelberg retina tomograph. (2007)
  • Author(s): N aka M, Kanamori A, Nakamura M, Negi A.
  • Organizer: 米国眼アカデミー
  • Place of Presentation: New Orleans.
• [Presentation] The regional relationship between retinal nerve fiber layer thickness and corresponding visual field sensitivity. (2007)
  • Author(s): Kanamori A, Naka M, Fujioka M, Nakamura M, Negi A.
  • Organizer: 米国眼アカデミー
  • Place of Presentation: New Orleans.
• [Presentation] ホームページ等
  • Author(s): http://www. med. kobe-u. ac. jp/ophthal/index. html