| Project/Area Number |
19390466
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
HARADA Hidemitsu Iwate Medical University, 歯学部, 教授 (70271210)
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| Co-Investigator(Kenkyū-buntansha) |
石関 清人 若手医科大学, 歯学部, 准教授 (50057775)
藤原 尚樹 若手医科大学, 歯学部, 講師 (20190100)
鍵谷 忠慶 若手医科大学, 歯学部, 助教 (30405774)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIZEKI Kiyoto 岩手医科大学, 歯学部, 准教授 (50057775)
FUJIWARA Naoki 岩手医科大学, 歯学部, 講師 (20190100)
KAGIYA Tadayoshi 岩手医科大学, 歯学部, 助教 (30405774)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Keywords | 歯の発生 / 象牙芽細胞 / リアルタイムイメージング / RECK / ROCK / 細胞極性 / マトリックスメタロプロテアーゼ / アメロゲニン / 発生・分化 / 歯学 / 細胞・組織 / シグナル伝達 / 歯胚発生 / Rhoシグナリング / real time imaging / slice culture / FGF9 / NGF / スライスカルチャー / ネスチン |
|---|
| Research Abstract |
We designed the real time imaging system in a slice tooth germ culture of actin promoter GFP transgenic mouse. Using the technique, we could capture the cell division and movement of mesenchymal cells in the dental papilla and demonstrate the formation of odontoblasts processes. We examined the role of Rho-associated kinase (ROCK) in odontoblasts differentiation using an organ culture of mouse incisor and mouse dental mesenchaml cell line. ROCK inhibitors and ROCK siRNA inhibited actin polymerization and E-cadherin expression on the cytoplasmic membrane. ROCK plays an important role in maintenance of cellular polarization through regulations of cytoskeleton and cell adhesion in the odontoblasts differentiation. Odotoblasts expressed the amelogenin, MMP inhibitors and MMP. Our data suggested that these factors play an important role in the maintenance of dentin canal.
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