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Molecular approaches to identify molecules involved in the cell aggregates formation in the skeletal blastema and elucidation of their functional properties

Research Project

Project/Area Number 19390475
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

NIFUJI Akira  Tsurumi University, 歯学部, 教授 (00240747)

Co-Investigator(Kenkyū-buntansha) SHIBATA Tatsuya  鶴見大学, 歯学部, 助教 (90323708)
荒木 良子  放射線医学総合研究所, 先端遺伝子発現研究グループ, 研究員 (40392211)
Co-Investigator(Renkei-kenkyūsha) ARAKI Ryoko  放射線医学総合研究所, 重粒子医科学センター, チームリーダー (40392211)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords薬理学 / 分化 / 遺伝子 / 発生 / 骨格形成 / 軟骨細胞 / 骨格
Research Abstract

During skeletal development, the skeletal blastema starts from cell aggregates formation in the mesoderm tissue. To understand skeletal tissue formation, it is crucial to elucidate molecular mechanism how cell aggregates are formed. In this study we aimed to establish molecular approaches to identify functional molecules involved in the cell aggregates formation and to analyze their expressions and functions. Through these approaches, we identified and characterized several molecules that may function in the skeletal blastema.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 2007

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (23 results) Book (2 results)

  • [Journal Article] Nemo-like kinase (NLK) expression in osteoblastic cells and suppression of osteoblastic differentiation2010

    • Author(s)
      Nifuji A, Ideno H, Ohyama Y, Takanabe R, Araki R, Abe M, Noda M, Shibuya H
    • Journal Title

      Exp Cell Res 316(7)

      Pages: 1127-36

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved methods for immunohistochemical detection of BrdU in hard tissue2010

    • Author(s)
      Nifuji A, et al
    • Journal Title

      Experimental Cell Research 316

      Pages: 1127-1136

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and cerberus (PRDC) inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro2009

    • Author(s)
      Ideno H, Takanabe R, Shimada A, Imaizumi K, Araki R, Abe M, Nifuji A
    • Journal Title

      Exp Cell Res 315(3)

      Pages: 474-84

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and cerberus (PRDC) inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro.2009

    • Author(s)
      Ideno H, et al
    • Journal Title

      Experimental Cell Research 315

      Pages: 474-484

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and cerberus (PRDC)inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro.2009

    • Author(s)
      Ideno H, et al
    • Journal Title

      Experimental Cell Research 315

      Pages: 474-84

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved methods for immunohistochemical detection of BrdU in hard tissue2008

    • Author(s)
      Shimada A, Shibata T, Komatsu K, Nifuji A
    • Journal Title

      Journal of Immunological Methods 339(1)

      Pages: 11-6

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth2008

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Shimoda S, Oida S, Kawasaki K, Nifuji A
    • Journal Title

      J Periodont Res 43(2)

      Pages: 194-200

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth2008

    • Author(s)
      Komatsu K, et al
    • Journal Title

      Journal Periodontal Research 43

      Pages: 194-200

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved methods for immunohistochemical detection of BrdU in hard tissue2008

    • Author(s)
      Shimada A, et al
    • Journal Title

      Journal Immunological Methods 339

      Pages: 11-6

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of CIZ, a nucleocytoplasmic shuttling protein, prevents unloading-induced bone loss through the enhancement of osteoblastic bone formation in vivo2007

    • Author(s)
      Hino K, Nakamoto T, Nifuji A, Morinobu M, Yamamoto H, Ezura Y, Noda M
    • Journal Title

      Bone 40(4)

      Pages: 852-60

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ihh/Gli2 Signaling Promotes Osteoblast Differentiation by Regulating Runx2 Expression and Function2007

    • Author(s)
      Shimoyama A, Wada M, Ikeda F, Hata K, Matsubara T, Nifuji A, Noda M, Amano K, Yamaguchi A, Nishimura R, Yoneda T
    • Journal Title

      Mol Biol Cell 18(7)

      Pages: 2411-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteopontin is required for mechanical stress-dependent signals to bone marrow cells2007

    • Author(s)
      Ishijima M, Tsuji K, Rittling SR, Yamashita T, Kurosawa H, Denhardt DT, Nifuji A, Ezura Y, Noda M
    • Journal Title

      J Endocrinology 193(2)

      Pages: 235-43

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] PlexinD1 deficiency induces defects in axial skeletal morphogenesis2007

    • Author(s)
      Kanda T, Yoshida Y, Izu Y, Nifuji A, Ezura Y, Nakashima K, Noda M
    • Journal Title

      J Cell Biochem 101(6)

      Pages: 1329-37

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] PlexinD1 deficiency induces defects in axial skeletal morphogenesis.2007

    • Author(s)
      Kanda T, Yoshida Y, Izu Y, Nifuji A, Ezura Y, Nakashima K, Noda M.
    • Journal Title

      J Cell Biochem 101

      Pages: 1329-37

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth2007

    • Author(s)
      Ishijima M, Tsuji K, Rittling SR, Yamashita T, Kurosawa H, Denhardt DT, Nifuji A, Ezura Y, Noda M.
    • Journal Title

      J Endocrinol 193(2):

      Pages: 235-43

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ihh/Gli2 Signaling Promotes Osteoblast Differentiation by Regulating Runx2 Expression and Function.2007

    • Author(s)
      Shimoyama A, Wada M, Ikeda F, Hata K, Matsubara T, Nifuji A, Noda M, Amano K, Yamaguchi A, Nishimura R, Yoneda T
    • Journal Title

      Mol Biol Cell. 18(7):

      Pages: 2411-8

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of CIZ, a nucleocytoplasmic shuttling protein, prevents unloading-induced bone loss through the enhancement of osteoblastic bone formation in vivo.2007

    • Author(s)
      Hino K, Nakamoto T, Nifuji A, Morinobu M, Yamamoto H, Ezura Y, Noda M.
    • Journal Title

      Bone 40(4)

      Pages: 852-60

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] The BMP antagonist PRDC promotes ALP activity in the absence of BMP2009

    • Author(s)
      出野尚、荒木良子、今泉和彦、安倍真澄、二藤彰
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] Masumi Abe Coordinate expression of multiple transcripts containing fragments of Line-1 retrotranposons during skeletal cell differentiation2009

    • Author(s)
      Akira Nifuji, Hisashi Ideno, Ryoko Araki
    • Organizer
      The 31st meeting of the American Society for Bone and Mineral Research (ASBMR)
    • Place of Presentation
      Denver Co
    • Related Report
      2009 Final Research Report
  • [Presentation] BMP antagonist PRDCの発現抑制による骨形成の促進2009

    • Author(s)
      出野尚、荒木良子、今泉和彦、安倍真澄、二藤彰
    • Organizer
      第64回体力医学会
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 骨再生を目的としたプラスミドDNAによるin vivo遺伝子導入のためのキャリアーの検討2009

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第27回骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨芽細胞分化の抑制・促進双方に働くBMP antagonist PRDCの機能2009

    • Author(s)
      出野尚、荒木良子、安倍真澄、二藤彰
    • Organizer
      第27回骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨芽細胞の生存と分化に及ぼすアレンドロネートの影響は分化段階により異なる2009

    • Author(s)
      小松浩一郎、島田明美、柴田達也、二藤彰
    • Organizer
      第27回骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] 生体吸収性材料とBMP-2プラスミドDNAの組合せによる頭蓋骨欠損修復の試み2009

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第8回日本再生医療学会総会、東京国際フォーラム
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨再生を目的としたプラスミドDNAによるin vivo遺伝子導入のためのキャリアーの検討2009

    • Author(s)
      柴田達也
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨芽細胞分化の抑制・促進双方に働くBMP antagonist PRDCの機能2009

    • Author(s)
      出野尚
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨芽細胞の生存と分化に及ぼすアレンドロネートの影響は分化段階により異なる2009

    • Author(s)
      小松浩一郎
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Coordinate expression of multiple transcripts containing fragments of Line-1 retrotranposons during skeletal cell differentiation2009

    • Author(s)
      二藤彰
    • Organizer
      第31回米国骨代謝学会ASBMR
    • Place of Presentation
      デンバー
    • Related Report
      2009 Annual Research Report
  • [Presentation] 1. 生体吸収性材料とBMP-2プラスミドDNAの組合せによる頭蓋骨欠損修復の試み2009

    • Author(s)
      柴田達也
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] 頭蓋骨欠損モデルを用いた安全な骨再生法の探究2008

    • Author(s)
      柴田達也
    • Organizer
      鶴見大学歯学会第68回例会
    • Place of Presentation
      横浜
    • Year and Date
      2008-12-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 硬組織におけるBrdU免疫組織化学の高感度化2008

    • Author(s)
      島田明美、柴田達也、小松浩一郎、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 再植歯周囲の骨リモデリングに及ぼす局所適用アレンドロネートの影響2008

    • Author(s)
      小松浩一郎、島田明美、柴田達也、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] プラスミドDNA遺伝子導入による骨再生の試み2008

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] プラスミドDNA遺伝子導入による骨再生の試み2008

    • Author(s)
      柴田達也
    • Organizer
      第26回日本骨代謝学会、
    • Place of Presentation
      大阪
    • Related Report
      2008 Annual Research Report
  • [Presentation] 骨再生を目指したプラスミドDNAによる頭蓋骨欠損修復モデルの確立2008

    • Author(s)
      荒木香映子
    • Organizer
      第21回日本歯科医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] 硬組織におけるBrdU免疫組織化学の最適化2007

    • Author(s)
      島田明美、柴田達也、小松浩一郎、二藤彰
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-31
    • Related Report
      2009 Final Research Report
  • [Presentation] アレンドロネート局所適用した再植歯周囲の骨密度と細胞増殖2007

    • Author(s)
      小松浩一郎、島田明美、柴田達也、二藤彰
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-30
    • Related Report
      2009 Final Research Report
  • [Presentation] Protein Related to DAN and Cerberus (PRDC) is expressed in skeletogenesis and prevents osteoblastic differentiation2007

    • Author(s)
      Nifuji A, Ideno H, Takanabe R, Shimada A, Araki R, Abe M
    • Organizer
      The 29th meeting of the American Society for Bone and Mineral Research (ASBMR)
    • Place of Presentation
      Honolulu, Hawaii
    • Related Report
      2009 Final Research Report
  • [Presentation] BMP抑制分子PRDCの骨芽細胞分化における発現、制御とその機能2007

    • Author(s)
      出野尚, 高鍋利依子, 荒木良子, 安倍真澄, 二藤彰
    • Organizer
      第25回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] Protein Related to DAN and Cerberus(PRDC)is expressed in skeletogenesis and prevents osteoblastic differentiation2007

    • Author(s)
      Nifuji A, Ideno H, Takanabe R, Shimada A, Araki R, Abe M
    • Organizer
      The 29th meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Honolulu
    • Related Report
      2007 Annual Research Report
  • [Book] 解る薬理学2008

    • Author(s)
      二藤彰、浜田節男、坂上宏、李昌一
    • Total Pages
      343
    • Publisher
      学建書院
    • Related Report
      2009 Final Research Report
  • [Book] 解る歯科薬理学2008

    • Author(s)
      二藤彰, 他
    • Total Pages
      343
    • Publisher
      学建出版
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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