A crosstalk between Toll-like receptor and C-type lectin

• Project/Area Number: 19390477
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Hokkaido University
• Principal Investigator: SHIBATA Kenichiro Hokkaido University, 大学院・歯学研究科, 教授 (50145265)
• Co-Investigator(Kenkyū-buntansha): HASEBE Akira 北海道大学, 大学院・歯学研究科, 助教 (90281815)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2007: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: 免疫 / 感染 / 炎症 / FSL-1 / TLR2 / DC-SIGN / XS106細胞 / リポアラビノマンナン / MDDC / Man_9(GLcNAc)_2

Research Abstract

Hosts recognize microbial invasion and then kill them intracellularily. In this study, we demonstrated that the signal mediated by a sensor that recognize bacterial invasion was suppressed by that by phagocytic receptor.

Research Products

• [Journal Article] The TLR2 ligand FSL-1 is internalized via clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2 (2010)
  • Author(s): HM Shamsul, A Hasebe, M Iyori, M Ohtani, K Kiura, D Zhang, Y Totsuka, K Shibata
  • Journal Title: Immunology 130 Pages: 262-272
  • NAID: 120002414898
• [Journal Article] The TLR2 ligand FSL-1 is internalized via clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2 (2010)
  • Author(s): HM.Shamsul, A.Hasebe, M.Iyori, M.Ohtani, K.Kiura, D.Zhang, Y Totsuka, K.Shibata
  • Journal Title: Immunology (In press)
  • NAID: 120002414898
  • Peer Reviewed
• [Journal Article] Effect of the antimicrobial peptide LL-37 on Toll-like receptor 2-, 3- and 4- triggered expression of IL-6, IL-8 and CXCL10 in human gingival fibroblasts (2010)
  • Author(s): Into T., Inomata M, Shibata K.
  • Journal Title: Mol.Oral Microbiol. (In press)
  • Peer Reviewed
• [Journal Article] Novel function of transcription factor ATF5 : blockade of p53-dependent apoptosis induced by ionizing irradiation. (2009)
  • Author(s): Nishioka T, Miyai Y, Haga H, Kawabata K, Shirato H, Homma A, Shibata K, Yasuda M.
  • Journal Title: Cell Struct Funct 34 Pages: 17-22
  • NAID: 130004137541
  • Peer Reviewed
• [Journal Article] A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli. (2008)
  • Author(s): M Iyori, M Ohtani, A Hasebe, Y Totsuka, K Shibata
  • Journal Title: Biochem. Biophy. Res. Comm 377(2) Pages: 367-372
  • NAID: 120002414904
• [Journal Article] Resveratrol modulates phagocytosis of bacteria through an NF-κB-dependent gene program (2008)
  • Author(s): M Iyori, H Kataoka, HM Shamsul, K Kiura, T Nakata, A Hasebe, M Yasuda, K Shibata
  • Journal Title: Antimicrob. Agents. Chemother 52(1) Pages: 121-127
  • NAID: 120002451617
• [Journal Article] Resveratrol modulates phagocytosis of bacteria through an NF-kB-dependent gene program (2008)
  • Author(s): M Iyori, H Kataoka, H M Shamsul, K Kiura, T Nakata, A Hasebe, M Yasuda, and K Shibata*
  • Journal Title: Antimicrobial Agents Chemotherapy 52 Pages: 121-127
  • Peer Reviewed
• [Journal Article] A role of the Ca^<2+> binding site of DC-SIGN in the phagocytosis of E. coli (2008)
  • Author(s): M Iyori, M Ohtani, A Hasebe, Y Totsuka and K Shibata
  • Journal Title: Biochem. Biophy. Res. Comm. 377 Pages: 367-372
  • NAID: 120002414904
  • Peer Reviewed
• [Journal Article] Regulation of MyD88-dependent signaling events by S-nitrosylation retards Toll-like receptor signal transduction and initiation of acute-phase immune responses (2008)
  • Author(s): Into T., Inomata M., Nakashima M., Shibata K., Hacker H., and Matsushita K.
  • Journal Title: Mol. Cell. Biol. 28 Pages: 1338-1347
  • Peer Reviewed
• [Journal Article] Reseveratrol modulates phagocytosis of bacteria through an NF-κB-dependent gene program. (2008)
  • Author(s): M Iyori, H Kataoka, H M Shamsul, K Kiura, T Nakata, A Hasebe, M Yasuda, and K Shibata*.
  • Journal Title: Antimicrobial Agents Chemotherapy 52
  • Peer Reviewed
• [Journal Article] The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway (2007)
  • Author(s): M Mae, M Iyori, M Yasuda, HM Shamsul, H Kataoka, A Hasebe, K Kiura, Y Totsuka, K Shibata
  • Journal Title: FEMS. Immunol. Med. Microbiol 49 Pages: 398-409
  • NAID: 120002451616
• [Presentation] Toll様受容体とC型レクチン受容体とのクロストーク (2010)
  • Author(s): 大谷誠
  • Organizer: 第83回日本細菌学会・総会
  • Place of Presentation: パシフィコ横浜、横浜市
• [Presentation] 9件)(1)柴田健一郎.マイコプラズマ由来リポタンパク質-リポペプチドのユニークな生物活性 (2009)
  • Author(s): 柴田健一郎
  • Organizer: 日本マイコプラズマ学会第36回学術集会講演要旨集:27
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-05
• [Presentation] How is the diacylated lipopeptide FSL-1processed after recognition by TLR2? (2009)
  • Author(s): MS Haque, A Hasebe, M Iyori, K Kiura, M Ohtani, K. Shibata
  • Organizer: he 96th Annual Meeting of The American Association of Immunologists
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2009-05-11
• [Presentation] Downregulation of the TLR2-mediated signal by the DC-SIGN ligand Man-LAM (2009)
  • Author(s): M Ohtani, A Hasebe, M Iyori, MS Haque, Y Totsuka, K Shibata
  • Organizer: The 96th Annual Meeting of The American Association of Immunologists
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2009-05-11
• [Presentation] Anti-and pro-tumor activities of the TLR2 ligand FSL-1 (2009)
  • Author(s): K Shibata, K Kiura, A Hasebe, M Iyori, MS Haque, M Ohtani
  • Organizer: The 96th Annual Meeting of The American Association of Immunologists
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2009-05-09
• [Presentation] Anti- and pro-tumor activities of the mycoplasmal lipopeptide FSL-1 as a TLR2 ligand. Innate Immunity Symposium (2008)
  • Author(s): Shibata K. Kiura K
  • Organizer: Regulation in Innate Immunity- from Recognition Molecules to Antimicrobial Peptides
  • Place of Presentation: Shionogi Innovation Center for drug discovery, Hokkaido University
  • Year and Date: 2008-10-28
• [Presentation] Toll-like receptor 2シグナルに及ぼすDC-SIGNシグナルの影響(優秀ポスター賞受賞) (2008)
  • Author(s): 大谷誠、伊従光洋、長谷部晃、木浦和人、戸塚靖則、柴田健一郎
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-24
• [Presentation] 腫瘍の増殖に及ぼすジアシルリポペプチドFSL-1の影響., 自然免疫の最前線-3学会合同大会2008- (2008)
  • Author(s): 木浦和人、柴田健一郎
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会・第19回日本生体防御学会学術総会・第45回補体シンポジウム
  • Place of Presentation: 札幌(北海道大学・学術交流会館)
  • Year and Date: 2008-07-10
• [Presentation] ジアシルリポペプチドFSL-1による制御性T細胞の増殖と腫瘍免疫応答の抑制 (2008)
  • Author(s): 木浦和人、長谷部晃、柴田健一郎
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-31
• [Presentation] Toll-like receptor 2とDC-SIGNシグナルのクロストーク (2008)
  • Author(s): 大谷誠、伊従光洋、長谷部晃、木浦和人、戸塚靖則、柴田健一郎
  • Organizer: 日本マイコプラズマ学会第35回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-30
• [Presentation] Toll様受容体とC型レクチン受容体とのクロストーク (2008)
  • Author(s): 円大谷誠
  • Organizer: 第50回歯科基礎医学会学術大会・総会
  • Place of Presentation: TOC有明、東京
• [Presentation] Toll様受容体と C型レクチン受容体とのクロストーク (2007)
  • Author(s): 柴田 健一郎
  • Organizer: 第49回歯科基礎医学会学術大会・総会
  • Place of Presentation: 北海道大学学術交流会館札幌
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/saikin/index.html