• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Clarification of the mechanisms of regeneration of periodontal ligament using human clonal periodontal ligament progenitor cell line

Research Project

Project/Area Number 19390486
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKyushu University

Principal Investigator

MAEDA Hidefumi  Kyushu University, 大学病院, 講師 (10284514)

Co-Investigator(Kenkyū-buntansha) AKAMINE Akifumi  九州大学, 大学院・歯学研究院, 教授 (00117053)
FUJII Shinsuke  九州大学, 大学病院, 助教 (60452786)
TOMOKIYO Atsushi  九州大学, 大学病院, 助教 (20507777)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Keywords歯内治療学 / ヒト歯根膜細胞 / 歯根膜幹細胞 / angiotensin / ヒト歯根膜幹細胞株 / 神経細胞 / NGF / カルシウム / BMP2 / 塩基性線維芽細胞増殖因子(bFGF) / SaOS2
Research Abstract

In this project, we have developed two clonal human periodontal ligament (PDL) stem/progenitor cell lines, and characterized them as cell elements responsible for PDL regeneration. Although both of cell lines expressed the same surface markers as bone marrow-derived mesenchymal stem cells, these cell lines specifically included Periostin and Scleraxis specific for PDL tissues. As scaffold elements, the materials containing calcium components effectively induced the differentiation of these two undifferentiated cell lines into cementoblastic/osteoblastic cells. Furthermore, as signaling elements, we clarified the efficacies of Basic Fibroblast Growth Factor and Transforming Growth Factor-β1 in PDL regeneration. And Angiotensin II that played important roles in regulating cardiovascular systems was suggested to affect tissue regeneration valuably. We also disclosed the functions of PDL stem/progenitor cells, indicating that our developed cell lines secreted Nerve Growth Factor to induce the neural differentiation, and neural migration, and inhibit apoptosis of neural cells. These results suggested that PDL tissue regeneration was accomplished by combining these three elements functionally.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (47 results)

All 2010 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (27 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Effect of MTAD on the differentiation of osteoblast-like cells2010

    • Author(s)
      Yasuda Y, Tatematsu Y, Fujii S, Maeda H, Akamine A, Torabinejad M, Saito T
    • Journal Title

      J Endod 36(2)

      Pages: 260-263

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human PeriodontalLigament Cells2010

    • Author(s)
      Maeda H, 他7名
    • Journal Title

      J Endod (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of MTAD on the differentiation of osteoblast-like cells2010

    • Author(s)
      Yasuda Y, 他6名
    • Journal Title

      J Endod 36

      Pages: 260-263

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MTAがヒト歯根膜線維芽細胞に及ぼす影響に関する研究2009

    • Author(s)
      前田英史, 友清淳, 藤井慎介, 島一也, 和田尚久, 門野内聡, 堀清美, 中野嗣久, 吉嶺嘉人, 赤峰昭文
    • Journal Title

      日歯保存誌 52(4)

      Pages: 355-362

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] MTAがヒト歯根膜線維芽細胞に及ぼす影響に関する研究2009

    • Author(s)
      前田英史, 他9名
    • Journal Title

      日歯保存誌 52

      Pages: 355-362

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 油症患者における歯周疾患ならびに口腔内色素沈着の疫学調査(第七報)2009

    • Author(s)
      橋口勇、吉嶺嘉人、前田英史、後藤康治、藤井慎介、友清淳、吉田桐枝、西垣奏一郎、門野内聡、堀清美、奥村英彦、赤峰昭文
    • Journal Title

      福岡医誌 100(5)

      Pages: 111-117

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミニマルインターベンションに基づく修復法の臨床応用に関する実態調査2009

    • Author(s)
      松下美樹子、畦森雅子、前田英史、坂井貴子、吉田桐枝、後藤康治、椛島浩明、赤峰昭文
    • Journal Title

      日歯保存誌 52(6)

      Pages: 483-492

    • NAID

      110007880696

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells.2008

    • Author(s)
      Yasuda Y, Inuyama H, Maeda H, Akamine A, Nor JE, Saito T
    • Journal Title

      J Oral Rehabil 35(12)

      Pages: 940-946

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways.2008

    • Author(s)
      Kano Y, Horie N, Doi S, Aramaki F, Maeda H, Hiragami F, Kawamura K, Motoda H, Koike Y, Akiyama J, Eguchi S, Hashimoto K
    • Journal Title

      Neurochem Res 33(9)

      Pages: 1795-803

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo.2008

    • Author(s)
      Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A
    • Journal Title

      J Cell Physiol 215

      Pages: 743-749

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multipotent clonal human periodontal ligament cell line.2008

    • Author(s)
      Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A
    • Journal Title

      Differentiation 76

      Pages: 337-347

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multipotent clonal human periodontal ligament cell line.2008

    • Author(s)
      Tomokiyo A, 他5名
    • Journal Title

      Differentiation 76

      Pages: 337-347

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo.2008

    • Author(s)
      Fujii S, 他5名
    • Journal Title

      J Cell Physiol 215

      Pages: 743-749

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways.2008

    • Author(s)
      Kano Y, 他11名
    • Journal Title

      Neurochem Res 33

      Pages: 1795-803

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multipotent clonal human periodontal ligament cell line2008

    • Author(s)
      Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A
    • Journal Title

      Differentiation (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo2008

    • Author(s)
      Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A
    • Journal Title

      J Cell Physiol (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells.

    • Author(s)
      Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A.
    • Journal Title

      J Endod (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] What are the characteristics of PDL stem cells? The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration: A View from Stem Cell Researches2010

    • Author(s)
      Maeda H, Fujii S, 他7名
    • Organizer
      Centennial Hall, Kyushu University School of Medicine
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-02-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Angiotensin II mediates the loading signal in human periodontal ligament cells.2010

    • Author(s)
      Monnouchi M, Maeda H, 他4名
    • Organizer
      The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration: A View from Stem Cell Researches"
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-02-05
    • Related Report
      2009 Final Research Report
  • [Presentation] What are the characteristics of PDL stem cells?2010

    • Author(s)
      Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada, Satoshi Monnouchi, Kiyomi Hori, Katsuaki Koori, Naohide Yamamoto, Akifumi Akamine
    • Organizer
      The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration : A View from Stem Cell Researches"
    • Place of Presentation
      Centennial Hall, Kyushu University School of Medicine, Fukuoka, Japan
    • Year and Date
      2010-02-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Angiotensin II mediates the loading signal in human periodontal ligament cells. The 5th International SAngiotensin II mediates the loading signal in human periodontal ligament cells2010

    • Author(s)
      Satoshi Monnouchi, Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Kiyomi Hori, Akifumi Akamine
    • Organizer
      The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration : A View from Stem Cell Researches"
    • Place of Presentation
      Centennial Hall, Kyushu University School of Medicine, Fukuoka, Japan
    • Year and Date
      2010-02-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Calcium-releasing Agent Exhibits Bioactive Effects in Endodontic Therapy.2009

    • Author(s)
      MAEDA H, TOMOKIYO A, 他5名
    • Organizer
      2nd World Conference on Magic Bullets (Ehrlich II)
    • Place of Presentation
      Nurnberg, Germany
    • Year and Date
      2009-10-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 歯根膜幹細胞はどんな特徴を持っているのだろうか?2009

    • Author(s)
      前田英史
    • Organizer
      第51回歯科基礎医学会サテライトシンポジウム
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター新潟市
    • Year and Date
      2009-09-09
    • Related Report
      2009 Final Research Report
  • [Presentation] 歯根膜幹細胞ばどんな特徴を持っているのだろうか?2009

    • Author(s)
      前田英史
    • Organizer
      第51回歯科基礎医学会
    • Place of Presentation
      新潟市
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Angiotensin II is involved in the loading signal in stretched human PDL cells.2009

    • Author(s)
      Monnouchi M, Maeda H, 他4名
    • Organizer
      3rd Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      Memorial Park, Hiroshima, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] AhRシグナルがヒト歯根膜細胞のコラーゲン代謝に及ぼす影響2009

    • Author(s)
      友清淳、前田英史, 他6名
    • Organizer
      第131回日本歯科保存学会秋季学術大会
    • Place of Presentation
      仙台
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] TGF-1がヒト歯根膜細胞および前駆細胞の増殖および分化に及ぼす影響2009

    • Author(s)
      藤井慎介、前田英史, 他6名
    • Organizer
      第130回日本歯科保存学会春季学術大会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] 伸展力が負荷されたヒト歯根膜細胞のシグナル伝達にはAngiotensin IIが関与している2009

    • Author(s)
      門野内聡、前田英史, 他4名
    • Organizer
      第130回日本歯科保存学会春季学術大会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] Expression and effects of TGFβ-1 in periodontal ligament tissue.2009

    • Author(s)
      S. FUJII, H. MAEDA, 他5名
    • Organizer
      87th General Session & Exhibition of the IADR/AADR/CADR
    • Place of Presentation
      MIAMI, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] MTA stimulates BMP2 expression in human PDL cells.2009

    • Author(s)
      H. MAEDA, A. TOMOKIYO, 他5名
    • Organizer
      87th General Session & Exhibition of the IADR/AADR/CADR
    • Place of Presentation
      2009 MIAMI, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] MTA stimulates BMP2 expression in human PDL cells.2009

    • Author(s)
      H.MAEDA, A.TOMOKIYO, S.FUJII, S.MONNOUCHI, N.WADA, K.HORI, A.AKAMINE
    • Organizer
      87th General Session & Exhibition of the IADR/AADR/CADR.
    • Place of Presentation
      MIAMI, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression and effects of TGFβ-1 in periodontal lig ament tissue.2009

    • Author(s)
      S.FUJII, H.MAEDA, N.WADA, A.TOMOKIYO, S.MONNOUSHI, K.HORI, A.AKAMINE
    • Organizer
      87th General Session & Exhibition of the IADR/AADR/CADR.
    • Place of Presentation
      MIAMI, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] 展力が負荷されたヒト歯根膜細胞のシグナル伝達にはAngiotensin IIが関与している2009

    • Author(s)
      門野内聡、前田英史、藤井慎介、友清淳、堀清美、赤峰昭文
    • Organizer
      第130回日本歯科保存学会春季学術大会
    • Place of Presentation
      札幌
    • Related Report
      2009 Annual Research Report
  • [Presentation] 1がヒト歯根膜細胞および前駆細胞の増殖および分化に及ぼす影響βTGF-2009

    • Author(s)
      藤井慎介、前田英史、友清淳、橋口勇、門野内聡、堀清美、和田尚久、赤峰昭文
    • Organizer
      第130回日本歯科保存学会春季学術大会
    • Place of Presentation
      札幌
    • Related Report
      2009 Annual Research Report
  • [Presentation] Angiotensin II is involved in the loading signal in stretched human PDL cells2009

    • Author(s)
      Satoshi Monnouchi, Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Kiyomi Hori, Akifumi Akamine
    • Organizer
      3rd Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      International Conference Center in Peace Memorial Park, Hiroshima. Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] MTAはヒト歯根膜細胞のBMP2発現を誘導する2008

    • Author(s)
      前田英史, 他8名
    • Organizer
      第129回日本歯科保存学会
    • Place of Presentation
      富山市
    • Year and Date
      2008-11-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] MTAが歯根膜細胞の分化に及ぼす影響に関する研究2008

    • Author(s)
      前田英史、友清淳, 他6名
    • Organizer
      第21回日本歯科医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] MTAはヒト歯根膜細胞のBMP2発現を誘導する2008

    • Author(s)
      前田英史、友清淳, 他7名
    • Organizer
      第129回日本歯科保存学会
    • Place of Presentation
      富山
    • Related Report
      2009 Final Research Report
  • [Presentation] MTA induces mineralization of human periodontal ligament cells2008

    • Author(s)
      H. MAEDA, A. TOMOKIYO, 他5名
    • Organizer
      86th General Session & Exhibition of the IADR/AADR/CADR
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2009 Final Research Report
  • [Presentation] A Multipotent hPDL Cell Line Promotes Neurocytic Differentiation and Migration.2008

    • Author(s)
      A. TOMOKIYO, H. MAEDA, 他4名
    • Organizer
      86th General Session & Exhibition of the IADR/AADR/CADR
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2009 Final Research Report
  • [Presentation] MTAはヒト歯根膜細胞の骨芽細胞様分化を誘導する.2008

    • Author(s)
      前田英史、中野嗣久, 他5名
    • Organizer
      第128回日本歯科保存学会
    • Place of Presentation
      新潟
    • Related Report
      2009 Final Research Report
  • [Presentation] 多分化能を有するヒト歯根膜クローン細胞株が神経細胞分化及び細胞遊走に及ぼす影響2008

    • Author(s)
      友清淳、前田英史, 他4名
    • Organizer
      第128回日本歯科保存学会
    • Place of Presentation
      新潟
    • Related Report
      2009 Final Research Report
  • [Presentation] 歯根膜組織の発生とヒト歯根膜2007

    • Author(s)
      前田英史
    • Organizer
      幹細胞株の樹立について再生歯科医学シンポジウム
    • Place of Presentation
      福岡市
    • Year and Date
      2007-04-12
    • Related Report
      2009 Final Research Report
  • [Presentation] EDTA、クエン酸または超音波を用いた根管洗浄後の根管象牙質のSEM観察.2007

    • Author(s)
      島一也、前田英史, 他6名
    • Organizer
      第28回日本歯内療法学会学術大会
    • Place of Presentation
      広島
    • Related Report
      2009 Final Research Report
  • [Book] 保存修復学専門用語集2009

    • Author(s)
      前田英史
    • Total Pages
      118
    • Publisher
      日本歯科保存学会編医歯薬出版
    • Related Report
      2009 Final Research Report
  • [Book] 医歯薬出版2009

    • Author(s)
      日本歯科保存学会
    • Total Pages
      118
    • Publisher
      保存修復学専門用語集
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等該当なし

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi