Analysis of proteins accumulated onto octacalcium phosphate and the related calcium phosphate crystals

• Project/Area Number: 19390490
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Osamu Tohoku University, 大学院・歯学研究科, 教授 (60374948)
• Co-Investigator(Kenkyū-buntansha): KAMAKURA Shinji 東北大学, 大学院・医工学研究科, 教授 (80224640) ; KAMIIE Junichi 麻布大学, 獣医学部, 講師 (10400269) ; ANMIIE Takahisa 東北大学, 大学院・歯学研究科, 助教 (30398466)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 生体材料 / リン酸オクタカルシウム(OCP) / 骨再生 / 結晶配向 / 成長因子

Research Abstract

Synthetic octacalcium phosphate (OCP) enhances bone formation and accumulates circulating serum proteins on its surface if implanted in bone defect. The rat serum proteins accumulated on OCP in vitro preparation were analyzed by proteomic approaches using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Over 100 proteins were detected from OCP surface as cellular and extracellular components. The extracellular components were categorized into molecular function, biological process and intracellular elements based on Protein ANalysis THrough Evolutionary Relationships classification system.

Research Products

• [Journal Article] Intramembranous bone tissue response to biodegradable octacalcium phosphate implant (2009)
  • Author(s): Kikawa T, Suzuki O, et al.
  • Journal Title: Acta Biomater 5(5) Pages: 1756-1766
  • Peer Reviewed
• [Journal Article] 市販β-TCP焼結体と比較した合成リン酸オクタカルシウム (OCP) の骨芽細胞の分化能 (2009)
  • Author(s): 川井忠, 穴田貴久, 鎌倉慎治, 鈴木治, 他
  • Journal Title: 日本口腔外科学会雑誌 56(1) Pages: 2-8
  • NAID: 10027841996
  • Peer Reviewed
• [Journal Article] The effect of microstructure of octacalcium phosphate on the bone regenerative property (2009)
  • Author(s): Honda Y, Anada T, Kamakura S, Suzuki O, et al.
  • Journal Title: Tissue Eng Part A 15(8) Pages: 1965-1973
  • Peer Reviewed
• [Journal Article] Synthetic octacalcium phosphate augments bone regeneration correlated with its content in collagen scaffold (2009)
  • Author(s): Kawai T, Anada T, Kamakura S, Suzuki O, et al.
  • Journal Title: Tissue Eng Part A 15(1) Pages: 23-32
  • Peer Reviewed
• [Journal Article] The effect of mcrostructure of octacalcium phosphate on the bone regenerative property (2009)
  • Author(s): Honda Y, 穴田貴久, 鎌倉慎治, 鈴木治, et al.
  • Journal Title: Tissue Eng Part A 15 Pages: 1965-1973
  • Peer Reviewed
• [Journal Article] 市販β-TCP焼結体と比較した合成リン酸オクタカルシウム(OCP)の骨芽細胞の分化能 (2009)
  • Author(s): 川井忠, 穴田貴久, 鎌倉慎治, 鈴木治, ほか
  • Journal Title: 日本口腔外科学会雑誌 56 Pages: 2-8
  • NAID: 10027841996
  • Peer Reviewed
• [Journal Article] Intramembranous bone tissue response to biodegradable octacalcium phosphate implant (2009)
  • Author(s): Kikawa T, 鈴木治, et al.
  • Journal Title: Acta Biomater 5 Pages: 1756-1766
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Suzuki O
  • Journal Title: Chemical and physical factors affecting osteoconductivity of octacalcium phosphate bone substitute material. Interface Oral Health Science 2009(In : Sasano T, Suzuki O, editors.)(Springer, Tokyo) Pages: 100-107
• [Journal Article] (2009)
  • Author(s): Fuji T, Anada T, Suzuki O, et al
  • Journal Title: Alginate/octacalcium phosphate composites enhance bone formation in critical-sized mouse calvaria. Interface Oral Health Science 2009(In : Sasano T, Suzuki O, editors.)(Springer, Tokyo) Pages: 308-310
• [Journal Article] Synthetic octacalcium phosphate augments bone regeneration correlated with its content in collagen scaffold (2009)
  • Author(s): Kawai T, Kamakura S, Suzuki O, et.al.
  • Journal Title: Tissue Eng Part A 15 Pages: 23-32
  • Peer Reviewed
• [Journal Article] Analysis of appositional bone formation using a novel rat experimental model (2008)
  • Author(s): Nakajima D, Kamakura S, Suzuki O, Sasano Y, et al.
  • Journal Title: Oral Dis 14(4) Pages: 308-313
  • Peer Reviewed
• [Journal Article] Oversulfated chondroitin sulfate-E binds to BMP-4 and enhances osteoblast differentiation (2008)
  • Author(s): Miyazaki T, Anada T, Suzuki O, et al.
  • Journal Title: J Cell Physiol 217(3) Pages: 769-777
  • Peer Reviewed
• [Journal Article] Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells (2008)
  • Author(s): Anada T, Kamakura S, Shimauchi H, Suzuki O, et al.
  • Journal Title: Tissue Eng Part A 14(6) Pages: 965-978
  • Peer Reviewed
• [Journal Article] Oversulfated chondroitin sulfate-E binds to BMP-4 and enhances osteoblast differentiation (2008)
  • Author(s): Miyazaki T, Anada T, Suzuki O, et.al.
  • Journal Title: J Cell Physiol 217 Pages: 769-777
  • Peer Reviewed
• [Journal Article] Analysis of appositional bone formation using a novel rat experimental model (2008)
  • Author(s): Nakajima D, Kamakura S, Suzuki O, Sasano Y, et.al.
  • Journal Title: Oral Dis 14 Pages: 308-313
  • Peer Reviewed
• [Journal Article] Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells (2008)
  • Author(s): Anada T, Kamakura S, Shimauchi H, Suzuki O, et. al.
  • Journal Title: Tissue Eng (印刷中)
  • Peer Reviewed
• [Journal Article] Stability of regenerated bone by octacalcium phosphate (OCP) combined with collagen (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et al.
  • Journal Title: Key Eng Mater 330-332 Pages: 1315-1318
  • Peer Reviewed
• [Journal Article] The primacy of octacalcium phosphate (OCP) collagen composites in bone regeneration (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et al.
  • Journal Title: J Biomed Mater Res A 83(3) Pages: 725-733
  • Peer Reviewed
• [Journal Article] Dehydrothermal treatment of collagen influences on bone regeneration by octacalcium phosphate (OCP) collagen composites (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et al.
  • Journal Title: J Tissue Eng Regen Med 1(6) Pages: 450-456
  • Peer Reviewed
• [Journal Article] Proliferation and adhesion of periodontal ligament cells on synthetic biominerals (2007)
  • Author(s): Hatakeyama J, Suzuki O, Sasano Y, et al.
  • Journal Title: Oral Dis 13(5) Pages: 500-506
  • Peer Reviewed
• [Journal Article] Dehydrothermal treatment of collagen influences on bone regeneration byoctacalcium phosphate(OCP)collagen composites (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et. al.
  • Journal Title: J Tissue Eng Regen Med 1 Pages: 450-456
  • Peer Reviewed
• [Journal Article] Proliferation and adhesion of periodontal ligament cells on synthetic biominerals (2007)
  • Author(s): Hatakeyama J, Suzuki O, Sasano Y, et. al.
  • Journal Title: Oral Dis 13 Pages: 500-506
  • Peer Reviewed
• [Journal Article] The primacy of octacalcium phosphate(OCP)collagen composites in bone regeneration (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et. al.
  • Journal Title: J Biomed Mater Res A 83 Pages: 725-733
  • Peer Reviewed
• [Journal Article] Octacalcium phosphate(OCP)combined with collagen orthotopocally enhances bone regeneration (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et. al.
  • Journal Title: J Biomed Mater Res B Appl Biomater 79 Pages: 210-217
  • Peer Reviewed
• [Journal Article] Stability of regenerated bone by octacalcium phosphate(OCP)combinedwith collagen (2007)
  • Author(s): Kamakura S, Anada T, Suzuki O, et. al.
  • Journal Title: Key Eng Mater 330-332 Pages: 1315-1318
  • Peer Reviewed
• [Presentation] リン酸カルシウム骨補填材による骨再生と骨置換促進のメカニズム (2009)
  • Author(s): 鈴木治
  • Organizer: 第424回東北大学整形外科談論会
  • Place of Presentation: 仙台(特別講演)
  • Year and Date: 2009-12-05
• [Presentation] リン酸カルシウム骨補填材による骨再生と骨置換促進のメカニズム (2009)
  • Author(s): 鈴木治
  • Organizer: 第424回東北大学整形外科談論会.特別講演
  • Place of Presentation: 仙台
  • Year and Date: 2009-12-05
• [Presentation] Osteoconductive and biodegradable characteristics of octacalcium phosphate (OCP) (2009)
  • Author(s): Suzuki O.
  • Organizer: Asia Core Program : Interdisciplinary Science of Nanomaterials : Session B "Nanobio & Carbon Nanotube Materials"
  • Place of Presentation: Sendai
  • Year and Date: 2009-09-25
• [Presentation] Osteoconductive and biodegradable characteristics of octacalcium phosphate (OCP) (2009)
  • Author(s): 鈴木治
  • Organizer: Asia Core Program : Interdisciplinary Science of Nanomaterials : Session B "Nanobio & Carbon Nanotube Materials" (招待講演)
  • Place of Presentation: Sendai
  • Year and Date: 2009-09-25
• [Presentation] Effect of topography of the octacalcium phosphate granule surfaces on its bone regenerative property (2009)
  • Author(s): Honda Y, Suzuki O, et al.
  • Organizer: Forsyth-Tohoku Symposium 2009
  • Place of Presentation: Boston MA, USA
  • Year and Date: 2009-03-10
• [Presentation] 高分子リンカーを有するリン酸カルシウム結合性 (2009)
  • Author(s): 穴田貴久, 鈴木治, ほか
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
• [Presentation] OCP顆粒インプラントの微細構造が骨形成に及ぼす影響 (2009)
  • Author(s): 本田義知, 穴田貴久, 鈴木治
  • Organizer: 第19回無機リン化学討論会
  • Place of Presentation: 東京
• [Presentation] リン酸オクタカルシウム (OCP) に集積するラット血清由来タンパク質のプロテオーム解析 (2009)
  • Author(s): 金子裕史, 上家潤一, 穴田貴久, 鎌倉慎治, 島内英俊, 鈴木治, ほか
  • Organizer: 第54回日本歯科理工学会学術講演会
  • Place of Presentation: 鹿児島
• [Presentation] リン酸オクタカルシウム(OCP)に集積するラット血清由来タンパク質のプロテオーム解析 (2009)
  • Author(s): 金子裕史, 上家潤一, 穴田貴久, 鎌倉慎治, 鈴木治, ほか
  • Organizer: 第54回日本歯科理工学会学術講演会
  • Place of Presentation: 鹿児島
• [Presentation] ヒドロキシアパタイト形成および骨形成における骨塩ミネラルの役割-アパタイト前駆体OCPが示す骨再生促進作用からの考察- (2008)
  • Author(s): 鈴木治
  • Organizer: 第50回歯科基礎医学会総会・学術大会, シンポジウム3
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] ヒドロキシアパタイト形成および骨形成における骨塩ミネラルの役割-アパタイト前駆体OCPが示す骨再生促進作用からの考察- (2008)
  • Author(s): 鈴木治
  • Organizer: 第50回歯科基礎医学会総会・学術大会, シンポジウム3(S3)
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Intramembranous bone reaction to synthetic OCP during hydroxyapatite deposition onto the OCP template (2008)
  • Author(s): Suzuki O, Kamakura S, et al.
  • Organizer: 8th World Biomaterials Congress (WBC2008)
  • Place of Presentation: Amsterdam, Netherland
  • Year and Date: 2008-05-30
• [Presentation] 合成リン酸オクタカルシウムの物理化学的性質と骨再生促進作用 (2008)
  • Author(s): 鈴木治
  • Organizer: 東北大学大学院工学研究科・機能高分子化学セミナー
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-02
• [Presentation] Release of β-Lactoglobulin adsorbed on octacalcium phosphate and its hydrolyzates (2007)
  • Author(s): Kumagai T, Anada T, Shimauchi H, Suzuki O, et al.
  • Organizer: International Dental Materials Congress 2007
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Biological response of octacalcium phosphate combined with collagen implanted in subperiosteal region (2007)
  • Author(s): Suzuki Y, Kamakura S, Anada T, Suzuki O, et al.
  • Organizer: International Dental Materials Congress 2007
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Effect of octacalcium phosphate ionic dissolution products on osteoblastic cell differentiation (2007)
  • Author(s): Anada T, Kamakura S, Suzuki O, et al.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes, France
• [Presentation] Differences of bone regeneration by various calcium phosphate/collagen composites (2007)
  • Author(s): Kamakura S, Anada T, Echigo S, Suzuki O, et al.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes, France
• [Presentation] Release of B-Lactoglobulin adsorbed on octacalcium phosphate and its hvdrolvzates (2007)
  • Author(s): Kumagai T, Anada T, Suzuki O, et. al.
  • Organizer: International Dental Materials Congress 2007
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Effect of octacalcium phosphate ionic dissolution products on osteoblastic cell differentiation (2007)
  • Author(s): Anada T, Kamakura S, Suzuki O, et. al.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes-France
• [Presentation] Differences of bone regeneration by various calcium phosphate/collagen composites (2007)
  • Author(s): Anada T, Kamakura S, Suzuki O, et. al.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes-France
• [Book] Alginate/octacalcium phosphate composites enhance bone formation in critical-sized mouse calvaria(Sasano T, Suzuki O, editors. Interface Oral Health Science 2009) (2009)
  • Author(s): Fuji T, Anada T, Suzuki O, et al.
  • Publisher: Springer
• [Book] Chemical and physical factors affecting osteoconductivity of octacalcium phosphate bone substitute material.(Sasano T, Suzuki O, editors. Interface Oral Health Science 2009) (2009)
  • Author(s): Suzuki O.
  • Publisher: Springer
• [Book] 生体模倣材料による生理的骨再生へのアプローチ (2008)
  • Author(s): 鈴木治, 鎌倉慎治, 穴田貴久, 本田義知
  • Publisher: ディカルレビュー社
• [Book] リン酸オクタカルシウム (2008)
  • Author(s): 鈴木治, 鎌倉慎治, 穴田貴久
  • Publisher: 日本医学館
• [Book] 「リン酸オクタカルシウムの骨再生能と生体内吸収性」セラミックス (2008)
  • Author(s): 鈴木治
  • Publisher: 日本セラミックス協会
• [Book] バイオマテリアル-生体材料-骨置換性材料「リン酸オクタカルシウム」, vol.26 No.3 (2008)
  • Author(s): 鈴木治, 鎌倉慎治, 穴田貴久
  • Total Pages: 7
  • Publisher: (株)日本医学館
• [Book] 再生医療、日本再生医療学会雑誌「生体模倣材料による生理的骨再生へのアプローチ」, Vol.7 No.2 (2008)
  • Author(s): 鈴木治, 鎌倉慎治, 穴田貴久, 本田義知
  • Total Pages: 6
  • Publisher: (株)メディカルレビュー社
• [Book] 「リン酸オクタカルシウムの骨再生能と生体内吸収性」セラミックス誌 (2008)
  • Author(s): 鈴木 治
  • Total Pages: 4
  • Publisher: 日本セラミックス協会
• [Book] Induction of octacalcium phosphate by surface modification of TiO2 film prepared by electron cyclotron resonance plasma oxidation : In Interface Oral Health Science 2007.(M. Watanabe, O. Okuno, editors) (2007)
  • Author(s): Orii Y, Suzuki O, et al.
  • Publisher: Springer
• [Book] Induction of octacalcium phosphate by surface modification of TiO_2 film prepared by electron cyclotron resonance plasma oxididation in"lntertace Oral Health Science 2007"eds M. Watanabe, O. Okuno, K. Sasaki, O. Suzuki, and N. Takahashi (2007)
  • Author(s): Orii Y, Suzuki O, et. al.
  • Publisher: Springer
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Remarks]
  • URL: http://www-kiso.dent.tohoku.ac.jp/cfe/index.html
• [Patent(Industrial Property Rights)] 骨再生材料およびその製造方法 (2008)
  • Inventor(s): 鈴木治, 他
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2008-04-16
• [Patent(Industrial Property Rights)] 骨再生材料およびその製造方法 (2008)
  • Inventor(s): 鈴木治, 他
  • Industrial Property Rights Holder: 東北大学, (株)ニプロ
  • Filing Date: 2008-04-16