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Investigation on the mechanisms of salivary gland tissue regeneration and effect of somatic stem cell transplantation

Research Project

Project/Area Number 19390512
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo

Principal Investigator

KAGAMI Hideaki  The University of Tokyo, 医科学研究所, 特任准教授 (80242866)

Co-Investigator(Kenkyū-buntansha) UEDA Minoru  名古屋大学, 大学院・医学系研究科, 教授 (00151803)
HONDA Masaki  東京大学, 医科学研究所・寄附研究部門, 教員 (70361623)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords唾液腺 / 再生 / 細胞培養 / 組織工学 / 遺伝子解析 / DNAマイクロアレイ / 細胞療法 / ディファレンシャルディスプレイ
Research Abstract

The regeneration of complex organ such as gland is still difficult. For the regeneration of organ, one of the most promising strategies is the acceleration of naturally existing regeneration capability. Although several approaches to enhance salivary gland regeneration have been reported, those cell based therapy are not sufficient so far. Accordingly, it was our aim to investigate a potential of salivary gland regeneration by use of somatic stem cells, which posses differentiating capability to virtually all salivary gland cells. First, we have investigated the effect of cell transplantation to irradiated salivary gland. Rat submandibular gland function was damaged after 20Gy irradiation. Allogenic mononuclear cells from bone marrow were transplanted to submandibular gland. The recovery of salivary gland secretion was observed and the treatment window was at least 1-14 days after transplantation. On the other hand, differentiation from mononuclear cells to parenchymal cells was not observed. Second, we have investigated the potential of stem cell culture from salivary gland, which can differentiate into all components of salivary gland. In this culture, possible salivary gland stem cells can be retained for relatively longer period and the cultured cells showed differentiation into acinar and duct. The possible trigger for the development has been investigated and the potential of autocrine or paracrine mechanisms were suggested.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (24 results)

All 2010 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (12 results) Book (3 results)

  • [Journal Article] A compact, automated cell culture system for clinical scale cell expansion from primary tissues.2010

    • Author(s)
      Kato R, Iejima D, Agata H, Asahina I, Okada K, Ueda M, Honda H, Kagami H.
    • Journal Title

      Tissue Eng Part C Methods (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Extracellular Matrix on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells into Smooth Musele Cell Lineage.2010

    • Author(s)
      Suzuki S, Narita Y, Yamawaki A, Murase Y, Satake M, Mutsuga M, Okamoto H, Kagami H, Ueda M, Ueda Y.
    • Journal Title

      Cells Tissues Organ (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel cell-based therapeutic strategy for ischemic colitis with use of bone marrow-derived mononuclear cells in rats.2009

    • Author(s)
      Tadauchi A, Narita Y, Kagami H, Niwa Y, Ueda M, Goto H
    • Journal Title

      Dis Colon Rectum 52

      Pages: 1443-1451

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restoring the function of salivary glands2008

    • Author(s)
      Kagami H, Songlin Wang, Bo Hai.
    • Journal Title

      Oral Diseases 14

      Pages: 15-24

    • Related Report
      2009 Final Research Report
  • [Journal Article] Restoring the function of salivary glands.2008

    • Author(s)
      Kagami H et al.
    • Journal Title

      Oral Diseases 24

      Pages: 15-24

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restoring the function of salivary glands2008

    • Author(s)
      Kagami H, et. al.
    • Journal Title

      Oral Diseases (in press)(掲載確定)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] A compact, automated cell culture system for clinical scale cell expansion from primary tissues

    • Author(s)
      Kato R, Iejima D, Agata H, Asahina I, Okada K, Ueda M, Honda H, Kagami H.
    • Journal Title

      Tissue Eng Part C Methods (in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Differential effects of growth differentiation factor-5 on porcine dental papilla-and follicle-derived cells

    • Author(s)
      Sumita Y, Honda MJ, Ueda M, Asahina I, Kagami H.
    • Journal Title

      Growth Factors (in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Limited but heterogeneous osteogenic response of human bone marrow mesenchymal stem cells to bone morphogenetic protein-2 and serum

    • Author(s)
      Mizuno M, Agata H, Furue H, Kimura A, Narita Y, Watanabe N, Ishii Y, Ueda M, Tojo A, Kagami H.
    • Journal Title

      Growth Factors (in press)

    • Related Report
      2009 Final Research Report
  • [Presentation] 唾液腺機能回復に対する新しいアプローチ~遺伝子治療と再生治療について~2010

    • Author(s)
      各務秀明
    • Organizer
      第12回神奈川県シェーグレン症候群研究会特別講演
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 体性幹細胞を用いた再生医療と顎口腔領域への応用2009

    • Author(s)
      各務秀明
    • Organizer
      第63回日本口腔科学会宿題報告
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2009-04-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス顎下腺を用いた新規無血清培養法の確立および器官形成能の検討2009

    • Author(s)
      青木玲奈, 縣秀樹, 大海忍, 各務秀明
    • Organizer
      第8回日本再生医療学会総会
    • Year and Date
      2009-03-06
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス顎下腺を用いた新規無血清培養法の確立および器官形成能の検討2009

    • Author(s)
      青木玲奈、縣秀樹、大海忍、各務秀明
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京国際フォーラム、東京
    • Year and Date
      2009-03-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス顎下腺を用いた新規無血清上皮培養法の開発2008

    • Author(s)
      青木玲奈, 縣秀樹, 大海忍, 各務秀明
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Year and Date
      2008-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス顎下腺を用いた新規無血清上皮培養法の開発2008

    • Author(s)
      青木玲奈、縣秀樹、大海忍、各務秀明
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートピアランド、神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] A novel serum-free culture method ofmouse submandibular gland epithelial cells2008

    • Author(s)
      Aoki R, Agata H, Imajoh-Ohmi S, -Kagami H
    • Organizer
      第22回唾液腺談話会
    • Year and Date
      2008-09-23
    • Related Report
      2009 Final Research Report
  • [Presentation] A novel serum-free culture method of mouse submandibular epithelial gland cells2008

    • Author(s)
      青木玲奈、縣秀樹、大海忍、各務秀明
    • Organizer
      第22回唾液腺談話会
    • Place of Presentation
      來京歯科大学、東京
    • Year and Date
      2008-09-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Tissue Engineering : Stem cells, growth factors and scaffold for tissue regeneration2008

    • Author(s)
      Kagami H.
    • Organizer
      Stem Cells and Clinic Applications Session II D : Clinical Translation & Disease Models Biobridge Event 2008
    • Place of Presentation
      United Nations, Geneva, Switzerland
    • Year and Date
      2008-09-22
    • Related Report
      2009 Final Research Report
  • [Presentation] (シンポウジウム (28) 細胞分化と口腔領域における再生)体性幹細胞の"stemness"について2008

    • Author(s)
      各務秀明, 縣秀樹, 水野大生, 上田実
    • Organizer
      第113回日本解剖学会総会
    • Year and Date
      2008-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] (シンポジウム2 口腔領域の Stem Cell Biology) 体性幹細胞の分化誘導と口腔組織再生への応用2007

    • Author(s)
      各務秀明, 本田雅規, 山田陽一, 水野裕和, 上田実
    • Organizer
      第61回日本口腔科学会総会
    • Year and Date
      2007-04-19
    • Related Report
      2009 Final Research Report
  • [Presentation] 体性幹細胞の分化誘導と口腔組織再生への応用2007

    • Author(s)
      各務 秀明、本田 雅規, 他
    • Organizer
      第61回日本口腔科学会総会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2007-04-19
    • Related Report
      2007 Annual Research Report
  • [Book] The Handbook of Intelligent Scaffold for Regenerative Medicine2010

    • Author(s)
      Kagami H, Agata H, Satake M, Narita Y
    • Publisher
      Pan Stanford Publishing(印刷中)
    • Related Report
      2009 Annual Research Report
  • [Book] 6-1. 口腔組織の再生・移植 (口腔内科学 尾崎登 喜雄編)2008

    • Author(s)
      各務秀明, 山本憲幸
    • Publisher
      飛鳥出版室
    • Related Report
      2009 Final Research Report
  • [Book] 口腔内科学2008

    • Author(s)
      各務秀明、山本憲幸
    • Total Pages
      464
    • Publisher
      飛烏出版室
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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