Molecular and genetic analysis of osteoclast differentiantion factor and osteoclastgenesis inhibitory factor in osteoporosis and control of inhibition of bone resorption by 1, 4-dihydroxy-2-naphthoic acid(DHNA)

• Project/Area Number: 19390516
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Aichi Gakuin University (2008); Okayama University (2007)
• Principal Investigator: SUGAHARA Toshio Aichi Gakuin University, 歯学部, 客員教授 (10116048)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Katsuaki 岡山大学病院, 口腔外科, 講師 (60304317) ; UENO Takaaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60252996) ; HIRATA Azumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (40263587) ; MATSUMURA Tatsushi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70432648) ; MINAMI Katsuhiro 愛知学院大学, 歯学部, 講師 (70346162) ; FUJIWARA Kumiko 愛知学院大学, 歯学部, 非常勤助教 (60404737) ; 山田 朋弘 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60335619)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2007: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
• Keywords: 骨粗鬆症 / ビフィズス菌 / プロピオン酸 / DHNA / 骨免疫 / 骨吸収 / 免疫 / 破骨細胞 / RANKL遺伝子

Research Abstract

免疫抑制剤FK506投与による骨粗懸症に対するプロピオン酸菌の発酵産物(PC)である新規なビフィズス菌増殖因子1, 4-dihydroxy-2-naphthoic acid(DHNA)の効果の検討した。
1. DHNAの骨粗鬆症改善効果を証明した。
2. この効果はFK506投与により増加したB細胞を減少させ、減少したT細胞を増加させることにより起こると考えられた。
3. 骨髄から、マイクロアレイを用いた網羅的解析を行ない、対照群と比較し発現差が認められたのは、骨免疫に関与している2487個の遺伝子であった。

Research Products

• [Journal Article] Application of a novel method to analyze lip motion to cleft lip patients before and after lip repair (2009)
  • Author(s): Mishima K., Yamada T., Sugii A., Matsumura T., Sugahara T.
  • Journal Title: Dentomaxillofacial Radiology (in press)
  • Peer Reviewed
• [Journal Article] Localization of Runx2, osterix and osteopontin in tooth root formation in Rat Molars (2009)
  • Author(s): Hirata, A., Sugahara, T. and Nakamura, H.
  • Journal Title: Journal of Histochemistry and Cytochemistry, (Epub ahead of print) 57 Pages: 397-403
  • Peer Reviewed
• [Journal Article] A histological comparison of bone induced from grafted periosteum with bone induced from grafted bone marrow in the rat calvarial defect model (2008)
  • Author(s): Ueno T, Honda K, Hirata A, Kagawa T, Kanou M, Shirasu N, Sugahara T
  • Journal Title: Acta Histochmeica 110(3) Pages: 217-223
  • Peer Reviewed
• [Journal Article] Hepkakenn ranase contributes to palate fusion by degradind perlecan (2008)
  • Author(s): A. Hirata, H. Imura, T. Yamada, T. Ueno, E. Yamachika, N. Moritani, T. Matsumura, T. Kagawa, K. Mishima, T. Sugahara
  • Journal Title: Jounal of Cranio-Maxillofacial Surgery Vol 36, (1) Pages: 173-173
  • Peer Reviewed
• [Journal Article] Expression of human β-defensin -1, -2, and -3 in non-inflamed pseudocyst, mucoceles (2008)
  • Author(s): MK Frederic, T Yamai, N Mizukawa, Y Kaneda, N Katase, M Gunduz, H Nagatsuka, T Sugahara
  • Journal Title: Oral Diseases 14 Pages: 652-657
  • Peer Reviewed
• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin in mice (2008)
  • Author(s): Fujiwara, K., Yamada, T., Mishima, K., Imura, H., Sugahara, T.
  • Journal Title: Congenital Anomalies 48(2) Pages: 68-73
  • NAID: 10027387216
  • Peer Reviewed
• [Journal Article] Relationships between nasalance scores and nasopharyngeal shapes in cleft palate patients (2008)
  • Author(s): Katsuaki Mishima, Tomohiro YAMADA, Asuka SUGII, Hideto IMURA and Toshio SUGAHARA
  • Journal Title: J Cranio-Maxillofacial Surgery 36(1) Pages: 11-14
  • Peer Reviewed
• [Journal Article] Dialectal and gender differences in nasalance scores in a Japanese population (2008)
  • Author(s): Katsuaki Mishima, Asuka SUGII, Tomohiro YAMADA, Hideto IMURA and Toshio SUGAHARA
  • Journal Title: J Cranio-Maxillofacial Surgery 2008b 36(1) Pages: 8-10
  • Peer Reviewed
• [Journal Article] Histological comparison of bone induced from autogenously grafted periosteum with bone induced from autogenously grafted bone marrow in the rat calvarial defect model (2008)
  • Author(s): Ueno, T., Honda, K., Hirata, A., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Yamachika, E., Mizukawa, N. and Sugahara, T.
  • Journal Title: Acta Histochmeica 110 Pages: 217-223
  • Peer Reviewed
• [Journal Article] Postoperative course after SSRO in mandibular asymmetries with or without MMF (2008)
  • Author(s): Tomohiro Yamada
  • Journal Title: Jounal of Cranio-Maxillofacial Surgery 36(1) Pages: 173-173
  • Peer Reviewed
• [Journal Article] Localization of Runx2, Osterix and Osteopontin in Tooth Root Formation in Rat Molars (2008)
  • Author(s): Azumi Hirata
  • Journal Title: J Histchetn & Cytochem (in press)
  • Peer Reviewed
• [Journal Article] Expression of human β-defensin -1, -2, and -3 in non-inflamed pseudocyst, mucoceles (2008)
  • Author(s): MK Frederic
  • Journal Title: Oral Diseases 14 Pages: 652-657
  • Peer Reviewed
• [Journal Article] 超高気孔率ハイドロキシアパタイトのラツト頭蓋骨骨欠損部での組織学的、X線学的観察-骨形成過程の予備的研究- (2008)
  • Author(s): 植野高章
  • Journal Title: 日本顎顔面インプラント学会雑誌 6(3) Pages: 205-211
  • Peer Reviewed
• [Journal Article] Relationships between nasalance scores and nasopharyngeal shapes in cleft palate patient (2008)
  • Author(s): Katsuaki Mishima
  • Journal Title: J Cranio -Maxillofacial Surgery 36(1) Pages: 11-14
  • Peer Reviewed
• [Journal Article] Functional requirement of CCN2 for intramembranous bone formation in embryonic mice (2008)
  • Author(s): Tomohiro Yamada
  • Journal Title: Biochem Biophys Res Commun 366(2) Pages: 450-456
  • Peer Reviewed
• [Journal Article] A Histological comparison of bone induced from grafted periosteum with bone induced from grafted bone marrow in the rat calvarial defect model (2008)
  • Author(s): Ueno T., Honda K., Hirata A., Kagawa T., Kanou M., Shirasu N., Sawaki M., Yamachika E., Mizukawa N., Sugahara T.
  • Journal Title: Acta Histochmeica E-pub(in press)
  • Peer Reviewed
• [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the development of the murine palate in organ culture (2007)
  • Author(s): Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Sugahara, T.
  • Journal Title: Asian J Oral Maxillofac Surg 19(4) Pages: 192-196
  • Peer Reviewed
• [Journal Article] The evaluation of bone formation of the whole tissue periosteum transplantation in combination with betatricalcium phosphate(TCP) (2007)
  • Author(s): Ueno. T., Sakata, Y., Hirata, A., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Fukunaga, J., Mizukawa, N. and Sugahara, T.
  • Journal Title: Annals of Plastic Surgery 59 Pages: 707-712
  • Peer Reviewed
• [Journal Article] Evaluation of Osteogenic Potential of Cultured Periosteum Derived Cells-Preliminary Animal Study- (2007)
  • Author(s): Ueno. T., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Imura, H., Hirata, A., Yamachika, E., Mizukawa, N. and Sugahara, T.
  • Journal Title: Journal of Hard Tissue Biology 16 Pages: 50-53
  • NAID: 110006401721
  • Peer Reviewed
• [Journal Article] Evaluation of osteogenic Potential of Cultured Periosteum Derived Cells-Preliminary Animal Study- (2007)
  • Author(s): Ueno T., Sakata Y., Hirata A., KagawazT., Kanou M., Shirasu N., Sawaki M., Mizukawa N., Sugahara T.
  • Journal Title: Journal of Hard Tissue Biology 16(2) Pages: 50-53
  • NAID: 110006401721
  • Peer Reviewed
• [Journal Article] Clinical investigation of dental implant reconstruction for grafted alveolar cleft patients. (2007)
  • Author(s): Sawaki M., Ueno T., Kagawa T., Kanou M., Sakata Y., Honda K., Shirasu N., Kuboki T., Sugahara T.
  • Journal Title: Journal of Hard Tissue Biology 16(2) Pages: 87-90
  • NAID: 110006401727
  • Peer Reviewed
• [Journal Article] The evaluation of bone formation of the whole tissue periosteum trasplantation in combination with beta-tricalcium phosphate (TCP) (2007)
  • Author(s): Ueno T., Sakata Y., Hirata A., Kagawa T., Kanou M., Shirasu N., Sawaki M., Fukunaga J., Mizukawa N., Sugahara T.
  • Journal Title: Annals of Plastic Surgery 59 Pages: 707-712
  • Peer Reviewed
• [Presentation] ヘパラナーゼは基底膜パールカンよりbFGFを遊離することで口蓋突起癒合に関与する (2008)
  • Author(s): 平田あずみ、井村英人、山田朋弘、植野高章、山近英樹、森谷徳文、松村達志、香川智正、三島克章、菅原利夫
  • Organizer: 第53回日本口腔外科学会総会
  • Place of Presentation: 徳島市
• [Presentation] Dental Implant treatment for the patients with Cleft Lip and Palate (2008)
  • Author(s): K. Minami, H. Imura, T. Niimi, H. Furukawa, K. Fujiwara, Y. Mori, T. Sugahara, N. Natsume
  • Organizer: 8th Asian Congress on Oral and Maxillogacial Surgery 2008
  • Place of Presentation: Thailand
• [Presentation] TCDD exposed fused palates had various phenomena (2008)
  • Author(s): H. Imura, T. Yamada, A. Hirata, K. Mishima, K. Minami, H. Furukawa, T., Niimi, K. Fujiwara, N. Natsume, T. Sugahara
  • Organizer: 8th Asian Congress on Oral and Maxillogacial Surgery 2008
  • Place of Presentation: Thailand
• [Presentation] Heparanase contributes to palate fusion by degragind perlecan (2008)
  • Author(s): A. Hirata, H. Imura, T. Yamada, T. Ueno, E. Yamachika, N. Moritani, T. Matsumura, T. Kagawa, K. Mishima, T. Sugahara
  • Organizer: 19th Congress of the European Association for Cranio- Maxillofacial Surgery
  • Place of Presentation: Bologna, Italy
• [Presentation] Osteogenic potential of grafted periosteum and bone marrow (2008)
  • Author(s): T. Ueno, A. Hirata, T. Kagawa, M. Wakita, M. Kanou, N. Shirasu, M. Sawaki, M. Moritani, Y. Yamachika, M. Mizukawa, T. Sugahara
  • Organizer: 19th Congress of the European Association for Cranio-Maxillofacial Surgery
  • Place of Presentation: Bologna, Italy
• [Presentation] Heparanase contributes to palate fusion by degragind perlecan (2008)
  • Author(s): Azumi Hirata
  • Organizer: 19th Congress of the European Association for Cranio-Maxillofacial Surgery
  • Place of Presentation: Bologna, Italy
• [Presentation] Dental Implant treatment for the patients with Cleft Lip and Palate (2008)
  • Author(s): Katsuhiro Minami
  • Organizer: 8^<th> Asian Congress on Oral and Maxillogacial Surgery 2008.
  • Place of Presentation: Bangkok, Thailand