Epigenetic investigation of oral squamous cell carcinoma -Application of epigenetic changes to cancer diagnosis and treatment-

• Project/Area Number: 19390520
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University
• Principal Investigator: SHINOHARA Masanori Kumamoto University, 医学薬学研究部, 教授 (90117127)
• Co-Investigator(Kenkyū-buntansha): HIRAKI Akimitsu 熊本大学, 大学院・医学薬学研究部, 講師 (60404034) ; NAKAYAMA Hideki 熊本大学, 医学部・附属病院, 助教 (70381001)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2007: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: DNAメチル化 / エピジェネティクス / がん抑制遺伝子 / CpGアイランド / 口腔扁平上皮癌 / 転移 / 薬剤耐性 / CGH分析 / 遺伝子解析 / メチル化

Research Abstract

癌においてDNAメチル化によるがん抑制遺伝子の不活化が重要な役割をなしていることが判ってきている。今回の研究で、癌細胞のDNAメチル化異常の検索で癌の存在や癌の性質の診断が可能となることが示唆された。また、正常組織に蓄積したDNAメチル化異常を検索することで発癌リスクを診断することが可能となることが示唆された。さらに、腫瘍細胞の転写抑制を回復させる脱メチル化剤による治療の可能性が示唆された。

Research Products

• [Journal Article] Significant Association Between Score of Periodontal Disease and Coronary Artery Disease (2009)
  • Author(s): Oe Y, Soejima H, Nakayama H, Fukunaga T, Sugamura K, Kawano H, Sugiyama S, Matsuo K, Shinohara M, Izumi Y, Ogawa H
  • Journal Title: Heart and Vessels 24 Pages: 103-107
  • Peer Reviewed
• [Journal Article] Aberrant expression of serum amyloid A in head and neck squamous cell carcinoma (2009)
  • Author(s): Shinriki S, Ueda M, Ota K, Nakamura M, Kudo M, Ibusuki M, Kim J, Yoshitake Y, Fukuma D, Jono H, Kuratsu JI, Shinohara M, Ando Y.
  • Journal Title: J Oral Pathol Med. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Humanized anti-interleukin-6 receptor antibody suppresses tumor angiogenesis and vivo growth of human oral squamous cell carcinoma (2009)
  • Author(s): Shinriki S., Jono H., Ota K., Ueda M., Kudo M., Ota T., Oike Y., Endo M., Ibusuk M., Hiraki A., Nakayama H., Yoshitake Y., Shinohara M., Ando Y.
  • Journal Title: Clin Cancer Res 15 Pages: 5426-5434
  • Peer Reviewed
• [Journal Article] Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recrutment to stalled replication forks (2008)
  • Author(s): Tsuji Y., Watanabe K., Araki K., Shinohara M., Tamagata Y., Tsurimoto T., Hanaoka F., Yamamura K., Yamaizumi M., Tateishi S.
  • Journal Title: Genes to Cell 13 Pages: 343-354
  • Peer Reviewed
• [Journal Article] Involvement of NF-kB-mediated of ADAM-17 in the invasion of oral squmous cell carcinoma (2008)
  • Author(s): Takamune Y, Ikebe T, Nagano O, Shinohara M
  • Journal Title: Biochem Biophys Res Commun 365 Pages: 393-398
  • Peer Reviewed
• [Journal Article] The role of DC-STAMP in maintenance of immune tolerance through regulation of dendritic cell function (2008)
  • Author(s): Sawatani Y., Miyamoto T., Nagai S., Maruya M., Imai J., Miyamoto K., Fujita N., Ninomiya K., Suzuki T., Iwasaki R., Toyama Y., Shinohara M., Koyasu S., Suda T.
  • Journal Title: Int Immunol 20(10) Pages: 1259-68
  • NAID: 10024357033
  • Peer Reviewed
• [Journal Article] Midkine as a prognostic biomaker in oral squamous cell carcinoma (2008)
  • Author(s): Ota K., Fujimori H., Ueda M., Shiniriki S., Kudo M., Jono H., Fukuyoshi Y., Yamamoto Y., Sugiuchi H., Iwase H., Shinohara M., Ando Y.
  • Journal Title: Br J Cancer 99 Pages: 655-662
  • Peer Reviewed
• [Journal Article] Periodontal Status And Prevotella Intermedia Antibody In Acute Coronary Syndrome (2008)
  • Author(s): Soejima H, Oe Y, Nakayama H, Matsuo K, Fukunaga T, Sugamura K, Kawano H, Sugiyama S, Shinohara M, Izumi Y, Ogawa H
  • Journal Title: Int J Cardiol (Epub ahead of print)
  • Peer Reviewed
• [Presentation] Inhibition of interleukin-6 receptor suppresses tumor growth of human oral squamous cell carcinoma cells by suppressing angiogenesis (2008)
  • Author(s): Shinriki S., Ota K., Jono H., Ueda M., Ota T., Kudo M., Sueyashi T., Shinohara M., Ando Y.
  • Organizer: 8^<th> International Conference of the Asian Clinical Oncology Society
  • Place of Presentation: Manila Philippine
  • Year and Date: 2008-09-21
• [Presentation] Midkine as a prognostic biomarker in oral squamous cell carcinoma (2008)
  • Author(s): Ota K., Fujimori H., Ueda M., Shinriki S., Kudo M., Jona H., Fukuyoshi Y., Ota T., Shinohara M., Ando Y.
  • Organizer: 8^<th> International Conference of the Asian Clinical Oncology Society
  • Place of Presentation: Manila Philippine
  • Year and Date: 2008-09-21
• [Presentation] Epigenetic regulation INK4/ARF focus by CTCF insulators (2008)
  • Author(s): Hirosue A., Ishihara K., Nakao M
  • Organizer: 2008 Global COR-IMEG Joint Summer Retreat Semina in ASO
  • Place of Presentation: Kumamoito JAPAN
  • Year and Date: 2008-08-28
• [Presentation] Preoperative concurrent chemoradiotherapy with S-1 for oral squamous cell carcinoma (2008)
  • Author(s): Nagata M., Namayama H., Nakahata A., Tanaka T., Hirosue A., Ota K., Namba A., Yashitake Y., Ota K., Obayashi T., Hiraki A., Shinohara M.
  • Organizer: 12^<th> International Congress on Oral Cancer
  • Place of Presentation: Shanghai China
  • Year and Date: 2008-05-22
• [Presentation] TNFa activates ADAM17 in order to cleve CD44 durung the invation of oral squamous cell carcinoma (2008)
  • Author(s): Takamune Y., Ikebe T., Yoshitake Y., Ogi H., Nakayama H., Ota K., Obayashi T., Hiraki A., Shinohara M.
  • Organizer: 58^<th> Congres of the Germany Society for Cranio-maxsillogacial Surgery
  • Place of Presentation: Munster Germany
  • Year and Date: 2008-05-14
• [Presentation] Relationship between expression of ADAM17 and CD44 cleavage in mechanism for the metastasis of orak squaqmous cell carcinoma (2007)
  • Author(s): Takamune Y., Ikebe T., Yoshitake Y., Ogi H., Nakayama H., Ota K., Obayashi T., Hiraki A., Shinohara M.
  • Organizer: American Association of Oral and Maxilloofacial Surgery 2007 Annual Meeting
  • Place of Presentation: Hawaii USA
  • Year and Date: 2007-10-08