Development of specific molecular target therapy for cancer using sonoporation and investigation for possibility of clinical application

• Project/Area Number: 19390522
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: FUKUDA Jin-ichi Kyushu Dental College, 歯学部, 特任教授 (00106270)
• Co-Investigator(Kenkyū-buntansha): NISHIHARA Tatsuji 九州歯科大学, 歯学部, 教授 (80192251) ; TOMINAGA Kazuhiro 九州歯科大学, 歯学部, 教授 (40188793) ; YOSHIOKA Izumi 九州歯科大学, 歯学部, 准教授 (10305823) ; HABU Manabu 九州歯科大学, 歯学部, 助教 (00360058) ; IWANAGA Kenjiro 九州歯科大学, 歯学部, 助教 (20448484)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2008: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 癌 / 遺伝子導入 / ソノポレーション / マイクロバブル / アポトーシス / ソノポレーション法 / 超音波導入法 / ナノバブル / 抗癌効果 / 口腔癌 / 扁平上皮癌細胞

Research Abstract

近年, 癌治療における薬剤, 遺伝子の有効性を高める方法として, ナノスケールの運搬体を利用して病巣に選択的に薬剤および遺伝子を送達することを目的とするターゲティング型DDS(Drug Delivery System)が注目を集めている. 我々は, 医療用に用いられる程度の出力の超音波と造影剤であるナノバブルを併用することにより, 癌細胞や癌組織に遺伝子や薬剤を導入する技術を見出し, 癌の特異的分子標的治療法の実現に向け多くの知見を得ることができた.

Research Products

• [Journal Article] Targeted drug delivery system for oral cancer therapy using sonoporation (2009)
  • Author(s): Hironobu Maeda, Kazuhiro Tominaga, Kenjiro Iwanaga, Fuminori Nagao, Manabu Habu, Toshiyuki Tsujisawa, Yuji Seta, Kuniaki Toyoshima, Jin-ichi Fukuda, Tatsuji Nishihara
  • Journal Title: Journal of Oral Pathology and Medicine (in press)
  • Peer Reviewed
• [Journal Article] The BMP signaling and its Smads in Mandibular Distraction Osteogenesis (2008)
  • Author(s): Khanal, A., Yoshioka, I., Tominaga, K., Furuta, N., Habu, M. and Fukuda, J.
  • Journal Title: Oral Diseases 14 Pages: 347-355
  • Peer Reviewed
• [Journal Article] Effects of latency on bone formation during the consolidation period of rabbit mandibular distraction (2008)
  • Author(s): Hayashi, K., Yoshioka, I., Khanal, A., Furuta, N., Tominaga, K., Fukuda, J.
  • Journal Title: Asian J Oral Maxillofac Surg 20(1) Pages: 5-11
  • Peer Reviewed
• [Journal Article] The BMP signaling and its Smads in Mandibular Distraction Osteogenesls. (2008)
  • Author(s): A, Khanal., J, Fukuda., et al.
  • Journal Title: Oral Diseases 14 Pages: 347-355
  • Peer Reviewed
• [Journal Article] Effects of latency on bone formation during the consolidation period of rabbit mandibular distraction. (2008)
  • Author(s): K, Hayashi., J, Fukuda., et al.
  • Journal Title: Asian J Oral Maxillofac Surg 20(1) Pages: 5-11
  • Peer Reviewed
• [Journal Article] Local delivery system of cytotoxic agents to tomor by focused sonoporation (2007)
  • Author(s): Iwanaga, K., et.Al.
  • Journal Title: Cancer Gene Therapy 14 Pages: 354-363
  • Peer Reviewed
• [Journal Article] Morphplogical features of cartilage during mandibular distraction in rabbits (2007)
  • Author(s): Furuta, N., et. al.
  • Journal Title: Int. J. Oral Maxillofac. Surg 36 Pages: 243-249
  • Peer Reviewed
• [Journal Article] Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasonography (2007)
  • Author(s): Tominaga, K., et. al.
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 409-411
  • Peer Reviewed
• [Presentation] Targeted drug delivery system for cancer therapy using sonoporation (2008)
  • Author(s): Maeda, H., Iwanaga, K., Nagao, F., Tsujisawa, T., Tominaga, K., Fukuda, J., Nishihara, T.
  • Organizer: 86^<st> General Session of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Gene transfer of interleukin-1 receptor antagonist into HIG-82 cells (2008)
  • Author(s): Nagao, F., Tujisawa, T., Maeda, H., Iwanaga, K., Habu, M., Yoshioka, I., Tominaga, K., Fukuda, J., Nishihara, T.
  • Organizer: 86^<st> General Session of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Targeted drug delivery system for cancer therapy using sonopor ation. (2008)
  • Author(s): H, Maeda., J, Fukuda., et al.
  • Organizer: 86^<st> General Session of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Gene transfer of interleukin-1 receptor antagonist into HIG-82cells. (2008)
  • Author(s): F, Nagao., J, Fukuda., et al.
  • Organizer: 86^<st> General Session of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] ソノポレーション法を用いて薬剤および遺伝子を導入したヒト扁平上皮癌に見られた細胞増殖抑制効果について (2007)
  • Author(s): 前田 博信, 他
  • Organizer: 第49回歯科基礎医学会総会
  • Place of Presentation: 札幌
• [Book] 口腔外科YEAR BOOK/一般臨床家、口腔外科医のための口腔外科ハンドマニュアル'07.特集3唇顎口蓋裂症例の長期経過観察 (2007)
  • Author(s): 佐藤 耕一, 福田 仁一
  • Total Pages: 2
  • Publisher: クインテッセンス出版2007.