Possible involvement of sulfation of heparan sulfate proteoglycan in tooth regeneration.

• Project/Area Number: 19390529
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMASHIRO Takashi Okayama University, 大学院・医歯薬学総合研究科, 教授 (70294428)
• Co-Investigator(Kenkyū-buntansha): SAITO Masahiro 大阪大学, 歯学研究科, 講師 (40215562) ; KAMIOKA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80253219)
• Co-Investigator(Renkei-kenkyūsha): HARADA Hidemitsu 岩手医科大学, 歯学部, 教授 (70271210)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2007: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: 糖鎖 / 歯 / プロテオグリカン / 再生 / 象牙質 / Sulf1 / Sulf2 / ヘパラン硫酸 / 上皮間葉相互作用 / sulf1 / sulf2 / 歯の発生 / 遺伝子発現 / ノックアウトマウス / 器官培養

Research Abstract

糖鎖は生体のシグナルとしてはたらくタンパク質を多彩に修飾し、その生理的機能および伝達を制御する。本研究の目的は、歯や硬組織における形態形成と分化における糖鎖の役割を明らかにすることである。ヘパラン硫酸プロテオグリカン硫酸基分解酵素であるSulf1とSulf2遺伝子に注目し、その発現部位、ノックアウトマウスの表現型、またin vitroの解析の結果から、糖鎖の修飾が少なくともWntシグナルの制御を介して、歯の形態形成と分化に関与していることが明らかとなった。

Research Products

• [Journal Article] Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4 (2010)
  • Author(s): Kawanabe N, Murata S, Murakami K, Ishihara Y, Hayano S, Kurosaka H, Kamioka H, Takano-Yamamoto T, Yamashiro T.
  • Journal Title: Differentiation 79 (2) Pages: 74-83
  • Peer Reviewed
• [Journal Article] Runx1 is involved in the fusion of the primary and the secondary palatal shelves (2009)
  • Author(s): Charoenchaikorn K, Yokomizo T, Rice DP, Honjo T, Matsuzaki K, Shintaku Y, Imai Y, Wakamatsu A, Takahashi S, Ito Y, Takano-Yamamoto T, Thesleff I, Yamamoto M, Yamashiro T.
  • Journal Title: Dev Biol. 15 ; 326 (2) Pages: 392-402
  • Peer Reviewed
• [Journal Article] characteristics in a patient with leprechaunism treated with insulin-like growth factor-I (2008)
  • Author(s): Fukunaga T, Murakami T, Tanaka H, Miyawaki S, Yamashiro T, Takano-Yamamoto T. Dental, craniofacial
  • Journal Title: Angle Orthod 78 (4) Pages: 745-51
  • Peer Reviewed
• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes (2008)
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Murshid SA, Hashimoto K, Kataoka N, Tsujioka K, Kajiya F, Yamashiro T, Takano-Yamamoto T.
  • Journal Title: Bone. 43 (1) Pages: 19-24
  • Peer Reviewed
• [Journal Article] Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria (2008)
  • Author(s): Ishihara Y, Kamioka H, Honjo T, Ueda H, Takano-Yamamoto T, Yamashiro T.
  • Journal Title: J Bone Miner Res. 23 (3) Pages: 350-60
  • Peer Reviewed
• [Journal Article] Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis (2007)
  • Author(s): Yamashiro T, Zheng L, Shitaku Y, Saito M, Tsubakimoto T, Takada K, Takano-Yamamoto T, Thesleff I.
  • Journal Title: Differentiation 75 (5) Pages: 452-62
  • Peer Reviewed
• [Presentation] The role heparan sulfate 6-0-endosulfatase Sulf1 and Sulf2 in tooth development (2010)
  • Author(s): Hayano S, Kurosaka H, Kalus I, Dierks T, Yamashiro T
  • Organizer: Gordon Research Confference-Craniofacial Biology-Craniofacial Morphogenesis & Tissue Regeneration
  • Place of Presentation: Lucca (Barga), Italy
• [Presentation] ヒト歯髄幹細胞の性質について (2009)
  • Author(s): 村田智子, 川邉紀章, 福島宏明, 山城隆
  • Organizer: 第68回日本矯正歯科学会大会
  • Place of Presentation: 福岡
• [Presentation] The role heparan sulfate 6-0-endosulfatase Sulf1 and Sulf2 in tooth development (2009)
  • Author(s): Hayano S, Kurosaka H, Kalus I, Dierks T, Yamashiro T
  • Organizer: Gordon Research Confference-Bone & Teeth
  • Place of Presentation: Biddeford, ME, USA
• [Presentation] 歯の発生におけるヘヘパラン硫酸の役割について (2008)
  • Author(s): 黒坂寛, 早野暁, 山城隆
  • Organizer: 第67回日本矯正歯科学会大会
  • Place of Presentation: 千葉
• [Patent(Industrial Property Rights)] 歯髄細胞から象牙芽細胞への分化誘導方法 (2009)
  • Inventor(s): 山城隆, 黒坂寛, 川邉紀章
  • Industrial Property Rights Holder: 国立大学法人 岡山大学
  • Industrial Property Number: 2009-262378
  • Filing Date: 2009-11-17
• [Patent(Industrial Property Rights)] 歯または歯周組織からの幹細胞の同定・単離方法ならびに該方法により得られる幹細胞 (2009)
  • Inventor(s): 川邉紀章, 山城隆
  • Industrial Property Rights Holder: 国立大学法人 岡山大学
  • Filing Date: 2009-08-04