Molecular genetics study of gutter-shaped root (GSR) in inbred mice using RNAi analysis.

• Project/Area Number: 19390534
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: ASADA Yoshinobu Tsurumi University, 歯学部, 教授 (20184145)
• Co-Investigator(Kenkyū-buntansha): IDAIRA Yayoi 鶴見大学, 歯学部, 助教 (40200018) ; INOUE Hiroko 鶴見大学, 歯学部, 講師 (50367306) ; MISHIMA Kenji 鶴見大学, 歯学部, 准教授 (50275343) ; SAITO Ichiro 鶴見大学, 歯学部, 教授 (60147634)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2007: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
• Keywords: 樋状根 / Real time PCR / Gene Chip / QTL解析 / Shh遺伝子 / Recombinant / inbred mice / マイクロアレイ法 / SHH pathway / AKXLマウス / 近交系マウス / Neuropeptide Y

Research Abstract

A detailed study using Quantitative trait locus (QTL) analysis, microarray and Real time PCR revealed that gutter-shaped root (GSR) formation in the C57L/J mice is strongly controlled by the genetic factor(s). It is well known that Shh signaling pathway is associated with the extension of the epithelial diaphragm (ED). It is suggested that the arrested development of ED in the C57L/J mice is caused by the fall of expression level of the Shh gene at 11days after birth. We concluded that GSR formationin the C57L/J mice is regulated by signaling factors such as Shh pathway.

Research Products

• [Journal Article] Genetic study of gutter-shaped root (GSR) in AKXL RI mouse strains using QTL analysis (2010)
  • Author(s): I. Tashima, K. Arita, Y. Asada
  • Journal Title: J Oral Science 52(2)(in printing)
  • NAID: 130000301257
  • Peer Reviewed
• [Journal Article] Quantitative trait locus analysis of gutter-shaped root(s) in C57L/J mouse (2010)
  • Author(s): K. Arita, I. Tashima, K. Ikeda, H. Nishimura, Y. Arai, I. Saito, Y. Asada
  • Journal Title: Pediatric Dent. J 20(1) Pages: 1-6
  • NAID: 10027485579
  • Peer Reviewed
• [Journal Article] Quantitative trait locus analysis of gutter-shaped root(s) in C57L/Jmouse (2010)
  • Author(s): Koichiro Arita, Itaru Tashima, Kenichi Ikeda, Hiroyuki Nishimura, Yoshinori Arai, Ichiro Saito, Yoshinobu Asada
  • Journal Title: Pediatric Dental Journal 20(1) Pages: 1-6
  • NAID: 10027485579
  • Peer Reviewed
• [Journal Article] Genetic study of gutter-shaped root(GSR)in AKXL RI mouse strains using QTL analysis (2010)
  • Author(s): Itaru Tashima, Koichiro Arita, Yoshinobu Asada
  • Journal Title: Journal of Oral Science 52(2)(in printing)
  • NAID: 130000301257
  • Peer Reviewed
• [Journal Article] A volume Evaluation of Dental Caries in a Mouse with the Use of the Micro-CT Scan (2008)
  • Author(s): Shinji Shimoda, Masumi Ohta, Yumna Mohideen, Tomoko Ohshima, Nobuko Maeda, Kenzo Kawasaki, Yoshinobu Asada
  • Journal Title: J Hard Tissue Biol 17(3) Pages: 109-114
  • NAID: 110007081304
  • Peer Reviewed
• [Journal Article] The Zinc transporter SLC39A13/ZIP13 is required for connective Tissue development; its involvement in BMP/TGF-βsignaling pathways (2008)
  • Author(s): Fukada T, Civic N, Furuichi T, Shimoda S, Mishima K, Higashiyama H, Idaira Y, Asada Y, Kitamura H, Yamasaki S, Hojyo S, Nakayama M, Ohara O, Koseki H, Santos, H.G. Bonafe L, Ha-Vinh R, Zankl A, Unger S, Kraenzlin, M.E. Beckmann J.S., Saito I, Rivolta C, IFukada T, Civic N, Furuichi T, Shim
  • Journal Title: PLos ONE 3(11)
  • Peer Reviewed
• [Journal Article] A volume evaluation of dental caries in a mouse with the use of the micro-CT scan (2008)
  • Author(s): Shinji Shimoda, Masumi Ohta, Yoshinobu Asada, et al.
  • Journal Title: Journal of Hard Tissue Biology 17(3) Pages: 109-114
  • NAID: 110007081304
  • Peer Reviewed
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-buccal surface of the mandibular first molar in microphthalmic mouse (2007)
  • Author(s): Tadafumi Tomohiro
  • Journal Title: Archives of Oral Biology 52 Pages: 828-835
  • Peer Reviewed
• [Presentation] 樋状根成因に対するsonic hedgehog signaling pathwayの関与について (2009)
  • Author(s): 田島格、有田浩一朗、伊平弥生、友広忠文、朝田芳信
  • Organizer: 第47回日本小児歯科学会学術大会
  • Place of Presentation: 大阪
• [Presentation] Zip13の歯根膜の恒常性とその再生における役割 (2009)
  • Author(s): 伊平弥生、下田信冶、深田俊幸、朝田芳信
  • Organizer: 第47回日本小児歯科学会学術大会
  • Place of Presentation: 大阪
• [Presentation] QTL解析を用いた樋状根成因に対する主働遺伝子の局在 (2007)
  • Author(s): 田島 格
  • Organizer: 第45回日本小児歯科学会大会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2007-07-19
• [Presentation] QTL解析を用いた樋状根成因に対する主導遺伝子の局在 (2007)
  • Author(s): 田島格、有田浩一朗、伊平弥生、古宇田ゆき、朝田芳信
  • Organizer: 第45回日本小児歯科学会学術大会
  • Place of Presentation: 東京