Impaired scaffold formation of AQP5 during aging : in the relation with mastication and swallowing

• Project/Area Number: 19390540
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: ISHIKAWA Yasuko The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 准教授 (40144985)
• Co-Investigator(Kenkyū-buntansha): OKAMURA Ko 大阪大学, 大学院・医学研究科, 助教 (50437381) ; SHONO Masayuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 技術職員 (60380101) ; 中江 良子 徳島大学, 大学院・ヘルスバイサイエンス研究部, 助教 (80127844)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 社会歯科学 / 水チャネル / アクアポリン / 老化 / ラフト / 咀嚼 / 嚥下 / 細胞内情報伝達系 / 基盤分子

Research Abstract

In order to spend a sane social life with the high quality even if we grow old, we investigate mechanism of pathogenesis of xerostomia caused by aging in relation with AQP5 and scaffold proteins. We developed a diagnostic kit of xerostomia using AQP5 in saliva. We examined whether the impaired mastication/swallowing ability linked decreasing the value that we obtained with the kit. The decreasing of AQP5 trafficking and the enhancement of AQP5 mistrafficking to basal plasma membrane were improved by administration of plant ceramide.

Research Products

• [Journal Article] Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva (2009)
  • Author(s): Yan Pan, Fusako Iwata, Di Wang, Masahiro Munaguchi, Keiko Ooga, Yasukazu Oomoto, Masaaki Takai, Gota Cho, Jinsen Kang, Masayuki Shono, Xue-jun Li, Ko Okamura, Toyoki Mori, Yasuko Ishikawa
  • Journal Title: Biochimica Et Biophysica Acta Vol.1790 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Correlation between salivary secretion and salivary AQP5 levels in health and disease. (2009)
  • Author(s): Di Wang, Fusako Iwata, Masahiro Muraguchi, Keiko Ooga, Yasukazu Ohmoto, Masaaki Takai, Toyoki Mori, Yasuko Ishikawa
  • Journal Title: he Journal of Medical Investigation Vol.56 Pages: 350-353
  • NAID: 130004822636
  • Peer Reviewed
• [Journal Article] Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva (2009)
  • Author(s): Pan Y,
  • Journal Title: Biochimica Et Biophysics Acta 1790 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Correlation between salivary secretion and salivary AQP5 levels in health and disease (2009)
  • Author(s): Wang D
  • Journal Title: The Journal of Medical Investigation 56 Pages: 350-353
  • Peer Reviewed
• [Journal Article] Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva (2009)
  • Author(s): Pan Y
  • Journal Title: Biochimica Et Biophysica Acta 1790 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Effect of iron-querceti n complex on reduction of nitrite in in vitro and in vivo systems. (2008)
  • Author(s): Hideki Ohnishi, Satoshi Iwanaga, Kazuyoshi Kawazoe, Keisuke Ishikawa, Sakiko Orino, Shuhci Tomita, Koichiro Tsuchiya, Yasuhisa Kanematsu, Nagakatsu Harada, Kazuhiro Mori, Tomoko Tsuchihnshi, Yasuko Ishikawa Toshiaki Tamaki
  • Journal Title: journal of Agricultural and Food Chemistry vol.56 Pages: 10092-10098
  • Peer Reviewed
• [Journal Article] Effect of iron-quercetin complex on reduction of nitrite in in vitro and in vivo system (2008)
  • Author(s): Ohnishi H
  • Journal Title: Journal of Agricultural and Food Chemistry 56 Pages: 10092-10098
  • Peer Reviewed
• [Journal Article] 水チャネル・アクアポリン (2007)
  • Author(s): 張 剛太
  • Journal Title: 日本薬理学雑誌 129 Pages: 136-137
  • Peer Reviewed
• [Presentation] Activation of M3 muscarinic receptors in rat parotid acinar cells induced AQP5 trafficking to nuclei and apical plasma membrane. (2010)
  • Author(s): Di Wang, Gota Cho, Ko Okamura, Yasuko Ishikawa
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-17
• [Presentation] Activation of α1-adrenoceptors inrat parotid acinar cells inducesAQP5 and GM1 trafficking toapicalplasma membrane and nuclei (2010)
  • Author(s): 王〓
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-03-17
• [Presentation] 唾液中の水チャネル・アクアポリン5のバイオマーカーとしての評価 (2010)
  • Author(s): 王〓, 岩田房子, 村口正宏, 大賀恵子, 大本安一, 高井正明, 森豊樹, 石川康子
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] Activation ofα 1-adrenoceptors in rat parotid acinar cells induced AQP5 and 01 trafficking to apical plasma membrane and nuclei. (2010)
  • Author(s): Di Wang, Gota Cho, Ko Okamura, Yasuko Ishikawa
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] Activation of M3 muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane (2010)
  • Author(s): 王〓
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-03-16
• [Presentation] The correlation between salivary secretion and salivary AQP5 levels in health and disease (2010)
  • Author(s): 王〓
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-03-16
• [Presentation] 耳下腺腺房細胞における水チャネル・AQP5の核と管腔膜への移動 (2009)
  • Author(s): 王〓, 石川康子
  • Organizer: 第51歯科基礎医学会学術大会総会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] 耳下腺腺房細胞における水チャネル・AQP5の核と管腔膜への移動 (2009)
  • Author(s): 王〓
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-10
• [Presentation] ムスカリン受容体刺激による唾液と唾液腺における水チャル・アクアポリンの動態 (2009)
  • Author(s): 石川康子
  • Organizer: 第51回歯科基礎医学会学術大会・サテライトシンポジウム
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-09
• [Presentation] The correlation between salivary secretion and salivary AQP5 levels in health and disease (2009)
  • Author(s): Wang D
  • Organizer: The 11^<th> International Symposium on Exocrine Secretion
  • Place of Presentation: 徳島市
  • Year and Date: 2009-07-25
• [Presentation] Correlation between salivary secretion and salivary AQP5 levels in health and disease. (2009)
  • Author(s): Di Wang, Fusako Iwata, Masahiro Muraguchi, Keiko Ooga, Yasukazu Ohmoto, Masaaki Takai, Toyoki Mori, Yasuko Ishikawa
  • Organizer: The 11th International Symposium on Exocrine Secretion (ISES)
  • Place of Presentation: Tokushima
  • Year and Date: 2009-07-24
• [Presentation] Association of salivary AQP5 and lipid rafts with salivary volume in men and women (2009)
  • Author(s): Di Wang, Ko Okamura, Yasuko Ishikawa
  • Organizer: 第82回目本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] 唾液中のAQP5や脂質・ラフトの濃度と唾液量の連関性 (2009)
  • Author(s): 王 〓
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] ムスカリン受容体刺激による水チャネル・アクアポリンの動態 (2009)
  • Author(s): 石川康子, 王〓, 岡村航
  • Organizer: 第51歯科基礎医学会学術大会・総会サテライトシンポジウム
  • Place of Presentation: 新潟
• [Presentation] ヒト分泌唾液中の水チャネル上アクアポリンと脂質・ラフトの検査指標としての価値 (2008)
  • Author(s): 石川康子, 王〓, 高嶋まゆ子, 岡村航
  • Organizer: 第53回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-06
• [Presentation] ヒト分泌唾液中の水チャネル・アクアポリンと脂質・ラフトの検査指標としての価値 (2008)
  • Author(s): 石川 康子
  • Organizer: 第53回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-06
• [Presentation] ヒト分泌唾液中の水チャネル・AQP5と脂質・ラフトの検査パラメーターとしての検討 (2008)
  • Author(s): 王〓, 石川康子
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Identification. of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimelne-stimulated saliva. (2008)
  • Author(s): Di Wang, Fusako Iwata Masahiro Munaguchi Keiko Ooga, Yasukazu Oomoto, Masaski Takai, Toyoki Mori, Yasuko Ishikawa
  • Organizer: The International Symposium on Oral Sciences to Improve the Quality of Life
  • Place of Presentation: Tokushima
  • Year and Date: 2008-09-06
• [Presentation] Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimelne-stimulated saliva (2008)
  • Author(s): Wang D
  • Organizer: The International Symposium on Oral Sciences to Improve the Quality of life
  • Place of Presentation: Tokushima
  • Year and Date: 2008-09-06
• [Presentation] シンポジウム「創薬ターゲットとしての水チャネル」ドライマウスの病態生理と治療薬 (2008)
  • Author(s): 石川 康子
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-26
• [Presentation] 唾液中AQP5と脂質・ラフトの動態 (2008)
  • Author(s): 康金森, 張剛太, 王〓, 石川康子
  • Organizer: 第81回日本薬理学会年会
  • Year and Date: 2008-03-19
• [Presentation] M3ムスカリン受容体刺激によるAQP5と脂質・ラフトの管腔膜と核への細胞内移動 (2008)
  • Author(s): 張剛太, 王〓, 康金森, 岡村航, 中條信義, 石川康子
  • Organizer: 第81回日本薬理学会年会
  • Year and Date: 2008-03-18
• [Presentation] M3ムスカリン受容体刺激によるAQP5とラフトの管腔膜と核への細胞内移動 (2008)
  • Author(s): 張 剛太
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-18
• [Presentation] 唾液中のAQP5と脂質・ラフトの動態 (2008)
  • Author(s): 康 金森
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-18
• [Presentation] 耳下腺腺房細胞における水チャネル・AQP5と脂質・ラフトの刺激による細胞内移動と唾液への遊離 (2007)
  • Author(s): 張剛太, 康金森, 王〓, 中條信義, 石川康子
  • Organizer: 第52回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2007-12-08
• [Presentation] ヒト分泌唾液中の水チャネル・AQP5と脂質ラフトの動態 (2007)
  • Author(s): 康金森, 張剛太, 岡村航, 中江良子, 石川康子
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 北海
  • Year and Date: 2007-08-30
• [Presentation] 耳下腺腺房細胞における水チャネル・AQP5と脂質ラフトの細胞内移動 (2007)
  • Author(s): 張剛太, 金森, 岡村航, 中江良子, 石川康子
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 北海道
  • Year and Date: 2007-08-30
• [Presentation] 耳下腺腺房細胞における水チャネル・AQP5と脂質・ラフトの刺激による細胞内移動 (2007)
  • Author(s): 張 剛太
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-30
• [Presentation] ヒト分泌唾液中の水チャネル・AQP5と脂質・ラフトの動態, (2007)
  • Author(s): 康 金森
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-30
• [Presentation] Impaired trafficking of AQP5 in Parotid glands of streptozotocin-induced diabetes. (2007)
  • Author(s): Zhenfang Yuan
  • Organizer: The 5^<th> International Conference of Aquaporin
  • Place of Presentation: Nara
  • Year and Date: 2007-07-15
• [Presentation] Dynamic trafficking of AQP5 together with lipid rafts in acinar cells ofrat parotid glands. (2007)
  • Author(s): Gouta Cho
  • Organizer: The 5^<th> International Conference of Aquaporin
  • Place of Presentation: Nara
  • Year and Date: 2007-07-14
• [Presentation] Alteration of AQP5 and lipid raft levels in basal and stimulated humansaliva. (2007)
  • Author(s): Yuan Pan
  • Organizer: The 5^<th> International Conference of Aquaporin
  • Place of Presentation: Nara
  • Year and Date: 2007-07-14
• [Book] からだと水の事典(佐々木成, 石橋賢一編) (2008)
  • Author(s): 石川康子, 大本安一, 高井正明, 森豊樹
  • Publisher: 朝倉書店
• [Book] からだと水の事典「口腔と唾液腺」 (2008)
  • Author(s): 石川 康子
  • Total Pages: 357
  • Publisher: 朝倉書店
• [Book] からだと水の事典 (2008)
  • Author(s): 石川 康子
  • Total Pages: 450
  • Publisher: 朝倉書店
• [Patent(Industrial Property Rights)] 唾液中の脂質・ラフトおよびAQP5を用いた唾液腺機能検査と全身疾患検査法 (2007)
  • Inventor(s): 石川康子, 大本安一, 高井正明, 森豊樹
  • Industrial Property Rights Holder: 国立大学法人徳島大学,大塚製薬株式会社
  • Filing Date: 2007-03-29