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Study of effect of oral microflora on oral and general health

Research Project

Project/Area Number 19390541
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionKyushu University

Principal Investigator

YAMASHITA Yoshihisa  Kyushu University, 大学院・歯学研究院, 教授 (20192403)

Co-Investigator(Kenkyū-buntansha) NAKANO Yoshio  九州大学, 大学院・歯学研究院, 准教授 (80253459)
SHIMAZAKI Yoshihiro  九州大学, 大学院・歯学研究院, 准教授 (10291519)
SHIOTA Susumiu  九州大学, 大学院・歯学研究院, 助教 (00150467)
KIYOHARA Yutaka  九州大学, 大学院・医学研究院, 教授 (80161602)
IIDA Mitsuo  九州大学, 大学院・医学研究院, 教授 (00127961)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords口腔フローラ / T-RFLP / 口腔疾患 / 全身の健康 / 16S rRNA遺伝子 / 網羅的解析 / 口腔の健康 / 口腔細菌叢 / 唾液検査
Research Abstract

In this study, oral microflora was examined in relationship to periodontitis and malodor, and was further analyzed with febrile days and pneumonia in institutionalized elderly. To characterize the composition of oral microflora, chromosomal DNA was extracted from bacteria in saliva or tongue coat and Terminal-Restriction Fragment Length Polymorphism analysis was carried out using the extracted bacterial DNA as template. The results indicated that T-RFLP pattern of salivary bacteria was closely associated with the prevalence of periodontitis and malodor. In addition, T-RFLP pattern in tongue coat was also associated with febrile days and pneumonia in institutionalized elderly.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (39 results)

All 2010 2009 2008 2007 Other

All Journal Article (11 results) (of which Peer Reviewed: 5 results) Presentation (22 results) Book (4 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Relationship between oral malordor and the global composition of indigenous bacterial populations in salaiva Appl Environ Microbiol.2010

    • Author(s)
      Takeshita T, Suzuki N, Nakano Y, Shimazaki Y, Yoneda M, Hirofqi T, Yamashita Y
    • Journal Title

      (in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] JAm Geriatr Soc2010

    • Author(s)
      Takeshita T, Tomioka M, Shimazaki Y, Matsuyama M, Koyano K, Matsuda K, Yamashita Y
    • Journal Title

      (in press)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Relationship between oral malodor and the global composition of indigen ous bacterial populations in saliva.2010

    • Author(s)
      Takeshita T, et al.
    • Journal Title

      Appl Eviron Microbiol (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfloral characterization of the tongue coating and associated risk for pneumonia-related health problems in the institutionalized elderly2010

    • Author(s)
      Takeshita T, et al.
    • Journal Title

      J Am Geriatr Soc (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status.2009

    • Author(s)
      Takeshita T, Nakano Y, Kumagai T, Yasui M, Kamio N, Shibata Y, Shiota S, Yamashita Y
    • Journal Title

      ISMEJ 3(1)

      Pages: 65-78

    • Related Report
      2009 Final Research Report
  • [Journal Article] The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status2009

    • Author(s)
      Toru Takeshita, et al.
    • Journal Title

      ISMEJ 3

      Pages: 65-78

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and application of a T-RFLP data analysis method using correlation coefficient matrices.2008

    • Author(s)
      Nakano Y, Takeshita T, Kamio N, Shiota S, Shibata Y, Yasui M, Yamashita Y
    • Journal Title

      JMicrobiol Methods 75(3)

      Pages: 501-505

    • Related Report
      2009 Final Research Report
  • [Journal Article] Development and application of a T-RFLP data analysis method using correlation coefficient matrices2008

    • Author(s)
      Nakano Y, et al.
    • Journal Title

      J Microbiol Methods 75

      Pages: 501-505

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improveded accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standarddefinition.2007

    • Author(s)
      Takeshita T, Nakano Y, Yamashita Y
    • Journal Title

      Oral Microbiol Immunol 22(6)

      Pages: 419-428

    • Related Report
      2009 Final Research Report
  • [Journal Article] 歯周病とメタボリックシンドローム2007

    • Author(s)
      山下 喜久
    • Journal Title

      公衆衛生 71

      Pages: 388-392

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standarddefinition2007

    • Author(s)
      Takeshita T, Nakano Y, Yamashita Y
    • Journal Title

      Oral Microbiology Immunology 22

      Pages: 419-428

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 唾液中に認められる細菌種の由来についての検討2010

    • Author(s)
      竹下徹、山中渉、山下喜久
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜市
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 口腔細菌叢と高齢者の健康2010

    • Author(s)
      山下喜久
    • Organizer
      第83回日本細菌学会総会ワークショップ
    • Place of Presentation
      横浜市
    • Year and Date
      2010-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 唾液中に認められる細菌種由来についての検討2010

    • Author(s)
      竹下徹、山中渉、山下喜久
    • Organizer
      日本細菌学会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 口腔細菌叢と高齢者の健康(ワークショップ)2010

    • Author(s)
      山下喜久
    • Organizer
      日本細菌学会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Relationship between the global composition of indigenous bacterial populations in saliva and oral malodor2010

    • Author(s)
      Takeshita T, Suzuki N, Nakano Y, Shimazakia Y, Yoneda M, Hirofuji T, Yamashita Y
    • Organizer
      International symposium on "oral health science" in 2010
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-02-06
    • Related Report
      2009 Final Research Report
  • [Presentation] 口腔フローラの構成の評価とその可能性2009

    • Author(s)
      竹下徹、山下喜久
    • Organizer
      第58回日本口腔衛生学会自由集会
    • Place of Presentation
      岐阜市
    • Year and Date
      2009-10-09
    • Related Report
      2009 Final Research Report
  • [Presentation] 口腔フローラの構成の評価とその可能性2009

    • Author(s)
      竹下徹、山下喜久
    • Organizer
      日本口腔衛生学会
    • Place of Presentation
      長良川国際学会場、岐阜市
    • Year and Date
      2009-10-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Comprehensive analysis of the bacterial flora of tongue coating and pneumonia risk in the institutionalized elderly2009

    • Author(s)
      Tomioka M, Takeshita T, Shimazaki Y, Matsuyama M, Koyano K, Yamashita Y
    • Organizer
      First European Congress on Microbial Biofilms
    • Place of Presentation
      Rome, Italia
    • Year and Date
      2009-09-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Comprehensive analysis of the bacterial flora of tongue coating and pneumonia risk in the institutionalized elderly2009

    • Author(s)
      Tomioka M, et al.
    • Organizer
      First European Congress on Microbial Biofilms
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2009-09-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高齢者における摂食状況と口腔細菌構成との関連性について2009

    • Author(s)
      冨岡未記子、竹下徹、嶋崎義浩、山下喜久
    • Organizer
      第31回日本口腔衛生学会九州地方会
    • Place of Presentation
      北九州市
    • Year and Date
      2009-07-05
    • Related Report
      2009 Final Research Report
  • [Presentation] 口腔細菌叢の総合解析に基づく未来型歯科口腔管理システムの構築2009

    • Author(s)
      山下喜久、中野善夫、廣藤卓雄、熊谷崇
    • Organizer
      第25回歯科医学を中心とした総合的な研究を推進する集い(平成20年度)
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] The change of microbial populations during plaque development2008

    • Author(s)
      Yasui M, Takeshita T, Nakano Y, Shimazaki Y, Shibata Y, Shiota S, AkifusaS, Yamashita Y
    • Organizer
      8th Congress of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Cheju, Korea
    • Year and Date
      2008-11-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Relationship between the tongue conditions and pyrexia in the elderly2008

    • Author(s)
      Tomioka M, Shimazaki Y, Takeshita T, MatsuyamaM, Koyano K, Yamashita Y
    • Organizer
      8th Congress of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Cheju, Korea
    • Year and Date
      2008-11-06
    • Related Report
      2009 Final Research Report
  • [Presentation] The change of microbial populations during plaque development2008

    • Author(s)
      Masaki Yasui, et al.
    • Organizer
      8th Congress of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Jeju Island, Korea
    • Year and Date
      2008-11-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] 口腔フローラのバランスと口腔の健康状態との関連性2008

    • Author(s)
      竹下徹、中野善夫、安井雅樹、熊谷崇、山下喜久
    • Organizer
      第50回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      札幌市
    • Year and Date
      2008-09-23
    • Related Report
      2009 Final Research Report
  • [Presentation] 口腔フローラのバランスと口腔の健康状態との関連性2008

    • Author(s)
      竹下徹ら
    • Organizer
      第50回歯科基礎医学会学術大会サテラィトシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2008-09-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] デンタルプラークの形成過程における細菌叢の変化について2008

    • Author(s)
      安井雅樹、中野善夫、柴田幸江、塩田進、秋房住郎、嶋崎義浩、竹下徹、山下喜久
    • Organizer
      第30回日本日腔衛生学会九州地方会
    • Place of Presentation
      大分市
    • Year and Date
      2008-07-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Correlation between oral malodor and bacterial composition in saliva2008

    • Author(s)
      Takeshital T, Suzuki N, Nakano Y, YonedaM, Shibata Y, Shiota S, Kamio N, Hirofuji T, Yamashita Y
    • Organizer
      86th General Session & Exhibition of the IADR
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2008-07-03
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] サポートベクターマシンを用いた唾液細菌叢分析に基づく口臭推定法2008

    • Author(s)
      中野善夫、竹下徹、神尾宜昌、柴田幸江、塩田進、鈴木奈央、米田雅裕、廣藤卓雄、山下喜久
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都市
    • Year and Date
      2008-03-24
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 相関行列を利用した多サンプルT-RFLP分析結果の「二次元」解析法2007

    • Author(s)
      中野善夫、竹下徹、山下喜久
    • Organizer
      第30回日本分子生物学会/第80回日本生化学会合同大会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] 相関行列を利用した多サンプルT-RFLP分析結果の「二次元」解析法2007

    • Author(s)
      中野 善夫、竹下 徹、山下 喜久
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] 口腔フローラが健康に及ぼす影響の解明2007

    • Author(s)
      竹下徹、中野善夫、柴田幸江、塩田進、嶋崎義裕、鈴木奈央、米田雅裕、廣藤卓雄、山下喜久
    • Organizer
      第29回日本口腔衛生学会九州地方会
    • Place of Presentation
      福岡市
    • Year and Date
      2007-07-08
    • Related Report
      2009 Final Research Report
  • [Book] Informatics in Oral Medicine : Advanced techniques in clinical and diagnostic technologies2010

    • Author(s)
      Edited by Andriani Daskalaki
    • Total Pages
      424
    • Publisher
      Medical Information Science Reference
    • Related Report
      2009 Annual Research Report
  • [Book] 新時代のプラークの病原性の評価2009

    • Author(s)
      山下喜久
    • Publisher
      末永書店
    • Related Report
      2009 Final Research Report
  • [Book] MI時代の歯科知識2009

    • Author(s)
      吉山昌宏、伊藤博夫、十河基文編集
    • Total Pages
      137
    • Publisher
      末永書店
    • Related Report
      2009 Annual Research Report
  • [Book] A comprehensive evaluation of the virulence of oral flora

    • Author(s)
      Yamashita Y, Takeshita T
    • Publisher
      Medical Information Science Reference, New York, USA
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 歯周病リスクの評価方法2010

    • Inventor(s)
      藤中英剛、坂本考司、中村純二、山下喜久、竹下徹
    • Industrial Property Rights Holder
      花王株式会社、国立大学法人九州大学
    • Industrial Property Number
      2010-064709
    • Filing Date
      2010-03-19
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] MICROBIAL COMMUNITY ANALYSIS2007

    • Inventor(s)
      山下 喜久, 他2名
    • Industrial Property Rights Holder
      花王株式会社
    • Filing Date
      2007-06-07
    • Related Report
      2007 Annual Research Report
    • Overseas

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Published: 2007-04-01   Modified: 2016-04-21  

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