Exploration of novel cold-adapted microorganisms to develop a systemfor the production of useful compounds at low temperatures

• Project/Area Number: 19404020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyoto University
• Principal Investigator: KURIHARA Tatsuo Kyoto University, 化学研究所, 准教授 (70243087)
• Co-Investigator(Kenkyū-buntansha): ESAKI Nobuyoshi 京都大学, 化学研究所, 教授 (50135597) ; MIHARA Hisaaki 京都大学, 化学研究所, 助教 (30324693)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 低温適応微生物 / 低温菌 / 好冷菌 / 低温物質生産 / 低温誘導性遺伝子 / タンパク質分泌生産

Research Abstract

アラスカ永久凍土やスイス高山土壌から低温での生育特性やタンパク質の分泌生産特性に優れた細菌を分離した。アラスカ永久凍土由来の低温適応微生物がヘモリシン共制御タンパク質のホモログを分泌高生産することを見いだし、本タンパク質の分泌装置を利用した外来タンパク質分泌生産系構築に向けて分泌機構を解析した。一方、南極海水から分離した低温菌について、本菌を宿主としたタンパク質生産系構築において利用価値が高いと考えられる低温誘導性プロモーターを単離した。

Research Products

• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium (2009)
  • Author(s): Kawamoto, J., Kurihara, T., Yamamoto, K., Nagayasu, M., Tani, Y., Mihara, H., Hosokawa, M., Baba, T., Sato, S. B. and Esaki, N.
  • Journal Title: Shewanella livingstonensis Ac10. J. Bacteriol 191 Pages: 632-40
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewannllla livingtlonnsiis Ac10 (2009)
  • Author(s): Kawamoto, Jun, et al.
  • Journal Title: J. Bacteriol. 191 Pages: 632-640
  • Peer Reviewed
• [Journal Article] Cold adaptation of eicosapentaenoic acid-less mutant of Shewanella livingstonensis Ac10 involving uptake and remodeling of synthetic phospholipids containing various polyunsaturated fatty acids (2008)
  • Author(s): Sato, S., Kurihara, T., Kawamoto, J., Hosokawa, M., Sato, S. B. and Esaki, N.
  • Journal Title: Extremophiles 12 Pages: 753-61
  • Peer Reviewed
• [Journal Article] Cold adaptation of eicosapentaenoic acid-less mutant of Shewanella livingstonensis Ac10 involving uptak and remodeling of synthetic phospholipids containing various polunmsaturated fatty acids (2008)
  • Author(s): Sato, Sho, et al.
  • Journal Title: Extremophiles 12 Pages: 753-761
  • Peer Reviewed
• [Journal Article] Proteomic studies of psychrophilic microorganisms (2008)
  • Author(s): Kurihara, Tatsuo, et al.
  • Journal Title: Psychrophiles : from Biodiversity to Biotechnology Pages: 333-344
• [Journal Article] Proteomic studies of psychrophilic microorganisms (2008)
  • Author(s): Kurihara, Tatsuo, et al.
  • Journal Title: Psychrophiles: from Biodiversity to Biotechnology Pages: 333-344
• [Journal Article] Proteomic studies of an Antarctic cold-adapted bacterium, Shewanella livingstonensis Ac10, for global identification of cold-inducible proteins (2007)
  • Author(s): Kawamoto, J., Kurihara, T., Kitagawa, M., Kato, I. and Esaki, N.
  • Journal Title: Extremophiles 11 Pages: 819-26
  • Peer Reviewed
• [Journal Article] Construction of a low-temperature protein expression system using a cold-adapted bacterium, Shewanella sp. strain Ac10, as the host (2007)
  • Author(s): Miyake, R., Kawamoto, J., Wei, Y.-L., Kitagawa, M., Kato, I., Kurihara, T. and Esaki, N.
  • Journal Title: Appl. Environ. Microbiol 73 Pages: 4849-56
  • Peer Reviewed
• [Journal Article] Construction of a low-temperature protein expression system using acold-adapted bacterium, Shewanella sp. strain Ac10, as the host (2007)
  • Author(s): Miyake, Ryoma, et al.
  • Journal Title: Appl. Environ. Microbiol 73 Pages: 4849-4856
  • Peer Reviewed
• [Journal Article] Proteomic studies of an Antarctic cold-adapted bacterium, Shewanella livingstonensis Ac10, for global identification of cold-inducible proteins (2007)
  • Author(s): Kawamoto, Jun, et al.
  • Journal Title: Extremophiles 11 Pages: 819-826
  • Peer Reviewed
• [Presentation] 低温菌 Shewanella livingstonensis Ac10のエイコサペンタエン酸生合成調節に関わる遺伝子orf4及びorfRの機能解析 (2009)
  • Author(s): 古賀敬太郎、川本純、栗原達夫、江崎信芳
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-29
• [Presentation] 海洋性低温菌 Shewanella livingstonensis Ac10 における高度不飽和脂肪酸の機能 (2008)
  • Author(s): 栗原達夫
  • Organizer: マリンバイオテクノロジー学会
  • Place of Presentation: 京都市
  • Year and Date: 2008-05-25
• [Presentation] Cold-adaptation mechanisms and, applications of an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2008)
  • Author(s): Kurihara, Tatsuo, et al.
  • Organizer: BIT Life Sciences' 1st Annual World Congress of iBio2008
  • Place of Presentation: 中華人民共和国・杭州
  • Year and Date: 2008-05-19
• [Presentation] プロモーター探索ベクターを用いた低温菌 Shewanella livingstonensis Ac10 の低温適応関連遺伝子の探索 (2008)
  • Author(s): 北山香織、三宅良磨、栗原達夫、江崎信芳
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-28
• [Presentation] 低温菌 Shewanella livingstonensis Ac10におけるエイコサペンタエン酸生合成の制御遺伝子 (2008)
  • Author(s): 古賀敬太郎、川本純、栗原達夫、江崎信芳
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-28
• [Presentation] Pseudomonas sp. Ak26 におけるHemolysin-coregulated protein ホモログの分泌機構の解析と応用 (2007)
  • Author(s): 小山大、栗原達夫、江崎信芳
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-14
• [Presentation] 低温菌Shewanella livingstonensis Ac10 の外膜透過性調節機構の解析 (2007)
  • Author(s): 川本純、栗原達夫、江崎信芳
  • Organizer: 第59回日本生物工学会大会
  • Place of Presentation: 東広島市
  • Year and Date: 2007-09-27
• [Presentation] Physiological role of phospholipids containing eicosapentaenoic acid in cold adaptation of an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2007)
  • Author(s): Kurihara, T.
  • Organizer: Gordon Research Conferences : Molecular and Cellular Biology of Lipids
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2007-07-24
• [Presentation] Physiological role of phospholipids containing eicosapentaenoic acid in cold adaptation of an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2007)
  • Author(s): Kurihara, Tatsuo, et al.
  • Organizer: Gordon Research Conferences: Molecular and Cellular Biology of Lipids
  • Place of Presentation: Waterville VaUey, NH, USA
  • Year and Date: 2007-07-24
• [Presentation] Cold-adaptation mechanisms and applications of an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2005)
  • Author(s): Kurihara, T.
  • Organizer: BIT Life Sciences' 1st Annual World Congress of iBio2008
  • Place of Presentation: 中華人民共和国・杭州
  • Year and Date: 2005-05-19
• [Book] P sychrophiles : from Biodiversity to Biotechnology (Edited by Margesin, R. et al., Springer) (2008)
  • Author(s): Kurihara, T. and Esaki, N.
  • Publisher: Proteomic studies of psychrophilic microorganisms