Transgenic trees for field trial in the world

• Project/Area Number: 19405030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: 林産科学・木質工学
• Research Institution: Kyoto University
• Principal Investigator: HAYASHI Takahisa Kyoto University, 応用生物科学部, 教授 (70231529)
• Co-Investigator(Kenkyū-buntansha): 谷口 亨 森林総合研究所, 森林バイオ研究センター, 研究室長 (00360470) ; 井出 雄二 東京大学, 農学生命科学研究所, 教授 (90213024) ; 渡邉 和男 筑波大学, 生命環境科学研究所, 教授 (90291806) ; 高部 鉄子 名古屋大学, 生命農学研究科, 教授 (60089852)
• Co-Investigator(Renkei-kenkyūsha): TANIGUCHI Toru 森林総合研究所, 森林バイオ研究センター, 研究員 (00360470) ; IDE Yuji 東京大学, 農学生命科学研究科, 教授 (90213024)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 組織構造 / 材形成 / 遺伝子組換え樹木 / 熱帯早生樹 / 生物多様性 / 環境政策 / 環境調和型農林水産 / 森林工学 / バイオテクノロジー / 植物

Research Abstract

The transgenic eucalyptus overexpressing xyloglucanase (AaXEG2)have been tested at the field of Israel and the transgenic mangium overexpressing xyloglucanase (AaXEG2) expects to be tested at the field of Indonesia. Critical analysis has been done in the field trials for transgenic trees in the world.

Research Products

• [Journal Article] Functions of xyloglucan in plant cells (2011)
  • Author(s): Hayashi T, Kaida R
  • Journal Title: Mol Plant Volume: 4 Pages: 17-24
  • Peer Reviewed
• [Journal Article] Enlargement of Individual cellulose microfibril in transgenic poplars overexpressing xyloglucanase. (2010)
  • Author(s): Yamamoto R, Saito T, Isogai A, Kurita M, Kondo T, Taniguchi T, Kaida R, Baba K, Hayashi T
  • Journal Title: J Wood Sci 57 Pages: 71-75
  • NAID: 10029035704
• [Journal Article] Functions of xyloglucan in plant cells. (2010)
  • Author(s): Hayashi T, Kaida R
  • Journal Title: Mol Plant 4 Pages: 17-24
• [Journal Article] Enhancing primary raw materials for biofuels. (2010)
  • Author(s): Hayashi T, Kaida R, Mistuda N, Ohme-Takagi M, Nishikubo N, Kidou S, Yoshida K
  • Journal Title: In Biomass to Biofuels(eds Vertes AA, Qureshi N, Blaschek HP, Yukawa H)(Wiley) Pages: 459-489
• [Journal Article] Loosening xyloglucan prevents tensile stress in tree stem bending but accelerates the enzymatic degradation of cellulose. (2010)
  • Author(s): Hayashi T, Kaida R, Kaku T, Baba K
  • Journal Title: Russian J Plant Physiol 57 Pages: 316-320
• [Journal Article] Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells (2010)
  • Author(s): Kaida R, Serada S, Norioka N, Norioka S, Neumetzler L, Pauly M, Sampedro J, Zarra I, Hayashi T, Kaneko TS
  • Journal Title: Plant Physiol 153 Pages: 603-610
• [Journal Article] Acceleration of cell growth by xyloglucan oligosaccharides in suspension-cultured tobacco cells (2010)
  • Author(s): Kaida R, Sugawara S, Negoro K, Maki H, Hayashi T, Kaneko TS
  • Journal Title: Mol Plant 3 Pages: 549-554
• [Journal Article] Sucrose synthase is an integral component of the cellulose synthesis machinery. (2010)
  • Author(s): Fujii S, Hayashi T, Mizuno K
  • Journal Title: Plant Cell Physiol 51 Pages: 294-301
  • NAID: 10027346948
• [Journal Article] Plasticity of xyloglucan composition in bean (Phaseolus vulgaris)-cultured cells during habituation and dehabituation to lethal concentrations of dichlobenil (2010)
  • Author(s): Alonso-Simon A, Neumetzler L, Garcia-Angulo P, Encina AE, Acebes JL, Alvarez JM, Hayashi T
  • Journal Title: Mol Plan 3 Pages: 603-609
• [Journal Article] Loosening xyloglucan prevents tensile stress in tree stem bending but accelerates the enzymatic degradation of cellulose (2010)
  • Author(s): Hayashi T, Kaida R, Kaku T, Baba K
  • Journal Title: Russian J Plant Physiol Volume: 57 Pages: 316-320
  • Peer Reviewed
• [Journal Article] Enlargement of individual cellulose microfibril in transgenic poplars overexpressing xyloglucanase (2010)
  • Author(s): Yamamoto R, Saito T, Isogai A, Kurita M, Kondo T, Taniguchi T, Kaida R, Baba K, Hayashi T
  • Journal Title: J Wood Sci Volume: 57 Pages: 71-75
  • NAID: 10029035704
  • Peer Reviewed
• [Journal Article] Proteomic analysis of the G-layer in poplar tension wood (2009)
  • Author(s): Kaku T, Serada S, Baba K, Tanaka F, Hayashi T
  • Journal Title: J.Wood Sci. 55 Pages: 250-257
  • NAID: 10025628364
• [Journal Article] Activation of β-glucan synthases by wall-bound purple acid phosphatase in tobacco cells. (2009)
  • Author(s): Kaida R, Satoh Y, Bulone V, Yamada Y, Kaku T, Hayashi T, Kaneko T
  • Journal Title: Plant Physiol 150 Pages: 1822-1830
• [Journal Article] Xyloglucan for generating tensile stress to bend tree stem. (2009)
  • Author(s): Baba K, Park WP, Kaku T, Kaida R, Takeuchi M, Yoshida M, Hosoo Y, Ojio Y, Okuyama T, Taniguchi T, Ohmiya Y, Kondo T, Shani J, Shoseyov O, Awano T, Serada S, Norioka N, Norioka S, Hayashi T
  • Journal Title: Mol Plant 2 Pages: 893-903
• [Journal Article] Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood. (2009)
  • Author(s): Kaida R, Kaku T, Baba K, Oyadomari M, Watanabe T, Nishida K, Kanaya T, Shani Z, Shoseyov O, Hayashi T
  • Journal Title: Mol Plant 2 Pages: 904-909
• [Journal Article] Enhancement of saccharification by overexpression of poplar cellulase in sengon. (2009)
  • Author(s): Kaida R, Kaku T, Baba K, Hartati S, Sudarmonowati E, Hayashi T
  • Journal Title: J Wood Sci 55 Pages: 435-440
  • NAID: 10027581441
• [Journal Article] Enzymatic saccharification and ethanol production of Acacia mangium and Paraserianthes falcataria wood, and oil palm trunk. (2009)
  • Author(s): Kaida R, Kaku T, Baba K, Hayashi T
  • Journal Title: J Wood Sci 55 Pages: 381-386
• [Journal Article] Enrichment of sugar content in melon fruits by hydrogen peroxide treatment (2009)
  • Author(s): K Ozaki, A Uchida, T Takabe, F Shinagawa, Y Tanaka, T Takabe, T Hayashi, T Hattori, AK.Raid, T Takabe
  • Journal Title: J Plant Physiol 166 Pages: 569-578
• [Journal Article] Enhancement of saccharification by overexpression of poplar cellulase in sengon (2009)
  • Author(s): Kaida, R., Kaku, T., Baba, K., Sri H., Enny S., T. Hayashi
  • Journal Title: J. Wood Sci. 55 Pages: 435-440
  • NAID: 10027581441
• [Journal Article] Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood (2009)
  • Author(s): Kaida, R., Kaku, T., Baba, K., Oyadomari, M., Watanabe, T., Nisida, K., Kanaya, T., Shani, Z., Shoseyov, O., Hayashi, T.
  • Journal Title: Mol. Plant 2(5) Pages: 904-909
• [Journal Article] Xyloglucan for generating tensile stress to bend tree stem. Mol. (2009)
  • Author(s): Baba, K., Park, Y.W., Kaku, T., Kaida, R., Takeuchi, M., Yoshida, M., Hosoo, Y., Ojio, Y., Okuyama, T., Taniguchi, T., Ohmiya, Y., Kondo, T., Shani, Z., Shoseyov, O., Awano, T., Serada, S., Norioka, N., Norioka, S., Hayashi, T.
  • Journal Title: Plant 2(5) Pages: 893-903
• [Journal Article] Enhancement of saccharification by overexpression of poplar cellulase in sengon (2009)
  • Author(s): Kaida R., et al.
  • Journal Title: J.Wood Sci. 55 Pages: 435-440
  • Peer Reviewed
• [Journal Article] Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment. (2008)
  • Author(s): T Hayashi, YW Park, A Isogai, T Nomura
  • Journal Title: J Wood Sci 54 Pages: 90-93
  • NAID: 10022025671
• [Journal Article] Biosafety assessment of transgenic poplars overexpressing xyloglucanase (AaXEG2) prior to field trials (2008)
  • Author(s): T Taniguchi, Y Ohmiya, M, Kurita, M Tsubomura, T Kondo, YW Park, K Baba, T Hayashi
  • Journal Title: J Wood Sci 54 Pages: 408-413
  • NAID: 10025388165
• [Journal Article] Purple acid phosphatase in the walls of tobacco cells (2008)
  • Author(s): R Kaida, T Hayashi, TS Kaneko
  • Journal Title: Phytochem 69 Pages: 2546-2551
  • NAID: 130006994079
• [Journal Article] Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants (2008)
  • Author(s): T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe
  • Journal Title: Plant Growth Reg 56 Pages: 265-273
• [Journal Article] Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon (2008)
  • Author(s): S Hartati, E Sudarmonowati E, YW Park YW, T Kaku, R Kaida, K Baba, T Hayashi
  • Journal Title: Plant Physiol 147 Pages: 552-561
• [Journal Article] Presence of xyloglucan-like polysaccharide in Spirogyra and possible involvement in cell-cell attachment (2008)
  • Author(s): H Ikegaya, T Hayashi, T Kaku, K Twata, S Sonobe, T Shimmen
  • Journal Title: Phycological Res 56 Pages: 216-222
  • NAID: 10024285208
• [Journal Article] Enhancement of saccharification by overexpression of various endoglycanase in poplar (2008)
  • Author(s): T Hayashi, R Kaida, T Kaku, K Baba
  • Journal Title: J Brasil Sci 55 Pages: 145-149
• [Journal Article] Xyloglucan : The molecular muscle of trees (2008)
  • Author(s): EJ Mellerowicz, P Immerzeel, T Hayashi
  • Journal Title: Annals Bot 10 Pages: 659-665
• [Journal Article] Cross-Linking of cell walls with dehydrated fructose by smoke-heat treatment (2008)
  • Author(s): T Hayashi, YW Park, A Isogai ahd T Nomura
  • Journal Title: Journal of Wood Science 54 Pages: 90-93
  • Peer Reviewed
• [Journal Article] Increase in XET activity in bean (Phaseolus vulgaris L.) cells habituated to dichlobenil (2007)
  • Author(s): A Alonso-Simon, P Garcia-Angulo, AE Encina, JM Alvarez, JL Acebes, T Hayashi
  • Journal Title: Planta 226 Pages: 765-771
• [Journal Article] Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar-A Glimpse into the Mechanism of the Balancing Act of Trees (2007)
  • Author(s): N Nishikubo, T Awano, A Banasiak, V Bourquin, F Ibatullin, R Funada, H Brumer, TT Teeri, T Hayashi, B Sundberg, EJ Mellerowicz
  • Journal Title: Plant Cell Physiol 48 Pages: 843-855
  • NAID: 10027002492
• [Journal Article] Structural organization and a standardized nomenclature for plant endo-1,4-β- glucanases (cellulases) of glycosyl hydrolase family 9. (2007)
  • Author(s): BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H Hofte, SJ McQueen-Mason, SE Patterson, O Shoseyov, TT Teeri, JKC Rose
  • Journal Title: Plant Physiology 144 Pages: 1693-1696
• [Journal Article] Structural organization and a standardized nomenclature for plant endo-1, 4-・-glcuanasses (cellulase) of (2007)
  • Author(s): BR Urbonowicz, AB Benett, E del Campillo, C Catala, T Hayashi, B
  • Journal Title: Plant Physiology 144 Pages: 1693-1696
  • Peer Reviewed
• [Journal Article] Xyloglucan Endo-transglycosylase (XET) Function in Gelatious Layers of Tension Wood Fibers in poplar-A (2007)
  • Author(s): N Nishikubo, T Awano, A Banasiak, V Bourquin, F
  • Journal Title: Plant Cell Physiol 48 Pages: 8443-855
  • Peer Reviewed
• [Presentation] Occurrence of xyloglucan in the cellulose microfibrils of the secondary wall (2010)
  • Author(s): Hayashi T, Kaida R
  • Organizer: XII Cell Wall Meeting
  • Year and Date: 2010-07-27
• [Presentation] 遺伝子組換え樹木の野外試験-細胞壁構造の改変 (2010)
  • Author(s): 林隆久
  • Organizer: 木材学会2009年度大会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2010-03-18
• [Presentation] Transgenic trees overexpressing AaXEG (2009)
  • Author(s): T Hayashi
  • Organizer: Plant Science and Genetic Seminar
  • Place of Presentation: the Hebrew University of Jerusalem
  • Year and Date: 2009-11-25
• [Presentation] Loosening xyloglucan accelerates the enzymatic hydrolysis of cellulose in wood (2009)
  • Author(s): T Hayashi
  • Organizer: Nissan International Tree Biology
  • Place of Presentation: Tokyo
  • Year and Date: 2009-02-26
• [Presentation] Proposed strategies and prospects for Riau biosphere reserve (2009)
  • Author(s): T Hayashi
  • Organizer: Workshop on Riau biosphere researve
  • Place of Presentation: Pekanbaru, Indonesia
  • Year and Date: 2009-02-20
• [Presentation] Transgenic poplars (2008)
  • Author(s): T Hayashi
  • Organizer: Plant feedstock seminar, Joint BioEnergy Institute
  • Place of Presentation: Emeryville, USA
  • Year and Date: 2008-12-15
• [Presentation] Transgenic poplars (2008)
  • Author(s): T Hayashi
  • Organizer: Plant biology seminar, Sao Paulo University
  • Place of Presentation: Sao Paulo, Brasil
  • Year and Date: 2008-12-12
• [Presentation] さまざまな細胞壁分解酵素を発現する組換えポプラ (2008)
  • Author(s): 林隆久、馬場啓一
  • Organizer: 理研シンポジウム
  • Place of Presentation: 理研横浜
  • Year and Date: 2008-02-18
• [Presentation] Enhancement of saccharification by overexpression of various endoglycanase in poplar (2008)
  • Author(s): T Hayashi
  • Organizer: International Symposium on Clean Energy for the World, Ethanol Biodiesel and Nayural Gas
  • Place of Presentation: Ubatuba, Brasil
• [Presentation] Current situation of researches on genetically modified trees in Japan (2007)
  • Author(s): 林隆久
  • Organizer: フィーラム:少資源国日本のバイオマス研究、日本分子生物学会生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Xyloglucan creates tensile stress in the secondary wall. (2007)
  • Author(s): T Hayashi, M.Takeuchi, Y.W.Park, T.Kaku, M.Yoshida, T.Awano, R.Kaida, K.Baba
  • Organizer: XI Cell Wall Meeting
  • Year and Date: 2007-08-15
• [Book] 森をとりもどすために2林木の育種 (2010)
  • Author(s): 林隆久編
  • Total Pages: 171
  • Publisher: 海青社、大津、滋賀
• [Book] 森をとりもどすために 2林木の育種 (2010)
  • Author(s): 林隆久
  • Total Pages: 171
  • Publisher: 海青社,大津市
• [Book] Biomass to Biofuels(Enhancing primary raw materials for biofuels) (2009)
  • Author(s): Hayashi T., Kaida R., Mistuda N., Ohme-Takagi M., Nishikubo N., Kidou S., Yoshida K.
  • Publisher: Wiley
• [Book] 森をとりもどすために (2008)
  • Author(s): 林隆久編
  • Total Pages: 102
  • Publisher: 海青社、大津、滋賀
• [Book] 森をとりもどすために (2008)
  • Author(s): 林隆久, 編集
  • Publisher: 海青社
• [Patent(Industrial Property Rights)] 糖化用植物および植物由来原料の調製方法 (2008)
  • Inventor(s): T Hayashi, K. Baba
  • Industrial Property Rights Holder: Kyoto University
  • Filing Date: 2008-08-25