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Comprehensive study to explore new physiologically active proteins in the snake venoms in the tropical and subtropical regions

Research Project

Project/Area Number 19406002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Biological pharmacy
Research InstitutionMeiji Pharmaceutical University

Principal Investigator

MORITA Takashi  Meiji Pharmaceutical University, 薬学部, 客員研究員 (90128108)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Yasuo  明治薬科大学, 薬学部, 助教 (30308621)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords生理活性 / 蛋白質 / プロテアーゼインヒビター / 神経毒蛋白質 / VEGF / タンパク質 / プロテオーム
Research Abstract

We have performed to study a diverse range of interesting proteins such as procoagulants, anticoagulants, platelet agonists, and platelet antagonists in the venoms of venomous snakes in the both tropical and sub-tropical region. In addition, we have comprehensively studied on the search, isolation, characterization of new C-type lectin-like proteins, cysteine-rich secretory proteins (CRISPs). We finally isolated and characterized more than ten structurally and functionally novel proteins with anticoagulant, antiplatelet or VEGF-activities. These molecules are known to exhibit potent and unique biological activities.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (53 results)

All 2010 2009 2008 2007

All Journal Article (22 results) (of which Peer Reviewed: 20 results) Presentation (24 results) Book (7 results)

  • [Journal Article] VKORの立体構造とワルファリンの作用機序2010

    • Author(s)
      森田隆司
    • Journal Title

      日本血栓止血学会誌

      Volume: 21 Pages: 395-362

    • NAID

      10026711741

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Snake venom vascular endothelial growth factors (VEGF-Fs) exclusively vary their structures and functions among species.2009

    • Author(s)
      Yamazaki Y, Matsunaga Y, Tokunaga Y, Obayashi S, Saito M, Morita T
    • Journal Title

      J.Biol.Chem. 284

      Pages: 9885-9891

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.2009

    • Author(s)
      Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
    • Journal Title

      Biochem.Biophys.Res.Commun. 379

      Pages: 872-875

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural divergence of cysteine-rich secretary proteins in snake venoms.2009

    • Author(s)
      Matsunaga Y, Yamazaki Y, Hyodo F, Nozaki M, Morita T
    • Journal Title

      J.Biochem. 145

      Pages: 365-375

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anticoagulant mechanism of factor IX/X-binding protein isolated frm the venom of Trimerresurus flavoviridis.2009

    • Author(s)
      Ishikawa M, Kumashiro M, Yamazaki Y, Atoda H, Morita T
    • Journal Title

      J.Biochem. 145

      Pages: 123-128

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Snake venom vascular endothelial growth factors (VEGF-Fs) exclusively vary their structures and functions among species2009

    • Author(s)
      Yamazaki, Y., Matsunaga, Y., Tokunaga, Y., Obayashi, S., Saito, M., Morita, T.
    • Journal Title

      J.Biol.Chem. 284

      Pages: 9885-9891

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Snake venom vascular endothelial growth factors(VEGF-Fs)exclusively vary their structures and functions among species2009

    • Author(s)
      Yamazaki Y, Matsunaga Y, Tokunaga Y, Obayashi S, Saito M, Morita T.
    • Journal Title

      J.Biol.Chem. 284

      Pages: 9885-9891

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.2009

    • Author(s)
      Matsunaga Y
    • Journal Title

      Biochem. Biophys. Res. Commun. 379

      Pages: 872-875

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Snake venom vascular endothelial growth factors (VEGF-Fs) exclusively vary their structures and functions among species.2009

    • Author(s)
      Yamazaki Y
    • Journal Title

      J. Biol. Chem. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytically inactive phospholipase A2 homologue binds to vcascular endothelial growth factor receptor-2 via a C-terminal loop region.2008

    • Author(s)
      Fujisawa D, Yamazaki Y, Lomonte B, Morita T
    • Journal Title

      Biochem.J. 411

      Pages: 515-522

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adenovirus serotype 5 hexon mediates liver gene transter.2008

    • Author(s)
      Waddington SN, McVey JH, Bhella D, Parker AL, Barker K, Atoda H, Pink R, Buckley SMK, Greig JA, Denby L, Custers J, Morita T, Francischetti IMB, Monteiro RQ, Barouch DH, Rooijen NV, Napoli C, Havenga MJE, Nicklin SA, Baker AH
    • Journal Title

      Cell 132

      Pages: 397-409

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels : implications for movement of the C-terminal cysteine-rich domain.2008

    • Author(s)
      Suzuki N, Yamazaki Y, Brown RL, Fujimoto Z, Morita T, Mizuno H
    • Journal Title

      Acta Cryst. D64

      Pages: 1034-1042

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adenovirus serotype 5 hexon mediates liver gene transter2008

    • Author(s)
      Waddington SN
    • Journal Title

      Cell 132

      Pages: 397-409

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytically inactive phospholipase A_2 homologue binds to vcascular endothelial growth factor receptor-2 via a C-terminal loop region2008

    • Author(s)
      Fujisawa D
    • Journal Title

      Biochem.J 411

      Pages: 515-522

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Augmentation of vascular permeability of VEGF is enhanced by KDR-binding proteins.2007

    • Author(s)
      Yamazaki Y, Nakano Y, Imamura T, Morita T
    • Journal Title

      Biochem.Biophys.Res.Commun. 355

      Pages: 693-699

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells2007

    • Author(s)
      Suzuki-Inoue K, Kato Y, Inoue O, Kaneko MK, Mishima K, Yatomi Y, Yamazaki Y, Narimatsu H, Ozaki Y
    • Journal Title

      J.Biol.Chem. 282

      Pages: 25993-26001

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Age-and gender-related differences of plasma prothrombin activity levels.2007

    • Author(s)
      Sakata T, Okamoto A, Morita T, Kokubo Y, Sato K, Okayama A, Tomoike H, Miyata T
    • Journal Title

      Thromb.Haemost. 97

      Pages: 1052-1053

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Augmentation of vascular permeability of VEGF is enhanced by KDR-binding proteins2007

    • Author(s)
      Yamazaki Y
    • Journal Title

      Biochem.Biophys.Res.Commun 355

      Pages: 693-699

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-and gender-related differences of plasma prothrombin activity levels2007

    • Author(s)
      Sakata T
    • Journal Title

      Thromb.Heamost 97

      Pages: 1052-1053

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Snake venom components affecting blood coagulation and the vascular system:Structural similarities and marked dicersity2007

    • Author(s)
      Yamazaki Y
    • Journal Title

      Current Pharmaceutical Design 13

      Pages: 2872-2886

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 血管内皮増殖因子ファミリー-その多様な構造と機能-2007

    • Author(s)
      山崎 泰男
    • Journal Title

      日本血栓止血学会誌 18

      Pages: 3-15

    • NAID

      10026114149

    • Related Report
      2007 Annual Research Report
  • [Journal Article] PIVKA-IIの生化学2007

    • Author(s)
      森田 隆司
    • Journal Title

      肝胆膵 54

      Pages: 459-463

    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗凝固薬の現状と将来の展望2010

    • Author(s)
      森田隆司
    • Organizer
      第4回 日本血流血管学会
    • Place of Presentation
      順天堂大学本郷キャンパス
    • Year and Date
      2010-11-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヘビ毒腺によってつくりだされる血液毒タンパク質の構造と機能2010

    • Author(s)
      森田隆司
    • Organizer
      第57回トキシンシンポジウム
    • Place of Presentation
      長浜ロイヤルホテル
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヘビ毒からの多彩な新規タンパク質の探索2010

    • Author(s)
      森田隆司
    • Organizer
      屋久島サテライトセミナー
    • Place of Presentation
      屋久島いわさきホテル
    • Year and Date
      2010-04-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規のKunitz型血液凝固Xa因子インヒビターの単離とその機能2010

    • Author(s)
      森田隆司
    • Organizer
      第33回 日本血栓止血学会 学術集会
    • Place of Presentation
      鹿児島市城山観光ホテル
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Snake venom vascular endothelial growth factors(VEGF-Fs) : Their structural similarities and marked diversities2009

    • Author(s)
      Morita T
    • Organizer
      4^<th> international conference on exogenous factors affecting thrombosis and haemostasis
    • Place of Presentation
      Boston, Massachusetts, USA
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Snake venom vascular endothelial growth factors (VEGF-Fs) : Their structural similarities and marked diversities2009

    • Author(s)
      Morita, T.
    • Organizer
      4^<th> International Conference on exogenous factors affecting thrombosis and haemostasis
    • Place of Presentation
      Boston, USA
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 血液凝固IX因子と抗凝固タンパク質(IX/X-bpおよびIX-bp)の相互作用に対するMg2+の影響2008

    • Author(s)
      石川みどり、山崎泰男、森田隆司
    • Organizer
      第55回毒素シンポジウム
    • Place of Presentation
      山梨
    • Related Report
      2010 Final Research Report
  • [Presentation] 組織型および毒型VEGF(血管内皮増殖因子)のゲノム構造解析2008

    • Author(s)
      山崎泰男、齋藤麻衣、徳永優子、森田隆司
    • Organizer
      第55回毒素シンポジウム
    • Place of Presentation
      山梨
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 血液凝固IX因子と抗凝固タンパク質(IX/X-bpおよびIX-bp)の相互作用の速度論的解析2008

    • Author(s)
      石川みどり、山崎泰男、森田隆司
    • Organizer
      日本薬学会128年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 異なるC末端ドメインを持つVEGFキメラの創製とその生物活性2008

    • Author(s)
      夘月博和、山崎泰男、森田隆司
    • Organizer
      日本薬学会128年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 組織型と毒型の血管内皮増殖因子のゲノム構造解析とその進化論的考察2008

    • Author(s)
      齋藤麻衣、徳永優子、長谷川芳裕、山崎泰男、森田隆司
    • Organizer
      日本薬学会128年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 血液・血管研究の新しい視点-毒タンパク質研究から何がわかるのか?-2007

    • Author(s)
      山崎 泰男
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2008)、ワークショップ「ベノミクス:毒生物ゲノムプロジェクト」
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] ハブ由来の組織型および毒型VEGF(血管内皮増殖因子)のゲノム構造解析2007

    • Author(s)
      齋藤 麻衣
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] ニューロピリン-1およびヘパリン結合性の異なる血管内皮増殖因子組換え体の調製2007

    • Author(s)
      夘月 博和
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗凝固活性を有すると報告された_L-アミノ酸参加酵素の抗凝固メカニズムの解析2007

    • Author(s)
      藤澤 大輔
    • Organizer
      第30回日本血栓止血学会学術集会
    • Place of Presentation
      三重
    • Year and Date
      2007-11-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Inactive Phospholipase A_2 Homologue Binds to VEGF Receptor-2 Via ItsC-Terminal Loo Reion2007

    • Author(s)
      Fujisawa D
    • Organizer
      The lst Seminar on Bioactive Natural Products forom Marine Organisms/EndophyticFungi and The JSPS 2nd Medicinal Chemistry Seminar of Asia/Afhca Science Platform Program
    • Place of Presentation
      Phuket,Thailand
    • Year and Date
      2007-10-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Use of Snake Venom Proteins in StUdies of the Stnlcture and Functionl of Coagulation Factors2007

    • Author(s)
      Morita T
    • Organizer
      The lst Seminar on Bioactive Natural Products from Marine Organisms/EndophyticFungi and The JSPS 2nd Medicinal ChemistrySeminar of Asia/Africa Science Platform Program
    • Place of Presentation
      Phuket,Thailand
    • Year and Date
      2007-10-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Trimieresurus flavoviridis毒腺cDNAライブラリーの網羅的解析2007

    • Author(s)
      長谷川 芳裕
    • Organizer
      第54回毒素シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2007-09-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Echis pyramidum毒由来の非ヘパリン結合性VEGFの単離とその性質2007

    • Author(s)
      乙部 清香
    • Organizer
      第54回毒素シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2007-09-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヘビ毒には多様な構造と性質をもつVEGFが含まれている2007

    • Author(s)
      松永 幸子
    • Organizer
      第54回毒素シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2007-09-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Augmentation of Vascular Permeability of Vascular Endothelial Growth Factor(VEGF) is Enhanced by VEGF Receptor 2-bindingProteins2007

    • Author(s)
      Yamazaki Y
    • Organizer
      XXIst Congress of the International Society on Thrombosis and Haemostasis, 53rd Annual SSC Meeting
    • Place of Presentation
      Geneva,Switzerland
    • Year and Date
      2007-07-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molecular and Functional Diversity of Snake Venom VEGFS2007

    • Author(s)
      Matsunaga Y
    • Organizer
      XXIst Congress of the International Society on Thrombosis and Haemostasis, 53rd Annual SSC Meeting
    • Place of Presentation
      Geneva,Switzerland
    • Year and Date
      2007-07-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of VEGF Receptor-binding Sites of KDR-binding Protein2007

    • Author(s)
      Fujisawa D
    • Organizer
      XXIst Congress of the International Society on Thrombosis and Haemostasis, 53rd Annual SSC Meeting
    • Place of Presentation
      Geneva,Switzerland
    • Year and Date
      2007-07-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] KDR結合タンパク質はVEGFの血管透過性元進作用を増強する2007

    • Author(s)
      山崎 泰男
    • Organizer
      第8回Phamlaco-Hematologyシンポジウム
    • Place of Presentation
      金沢
    • Year and Date
      2007-06-08
    • Related Report
      2007 Annual Research Report
  • [Book] Toxins and Hemostasis.2010

    • Author(s)
      Kini RM, Clemetson KJ, Markland FS, Mclane MA, Morita T (Editors.)
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Toxins and Hemostasis2010

    • Author(s)
      Takashi Morita (Editor)
    • Total Pages
      797
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Book] 日本血栓止血学会誌(血液凝固X因子-その周辺と展望-, 20巻5号)2009

    • Author(s)
      森田隆司
    • Total Pages
      7
    • Related Report
      2009 Self-evaluation Report
  • [Book] 血液凝固X因子 -その周辺と展望-2009

    • Author(s)
      日本血栓止血学会誌
    • Total Pages
      7
    • Publisher
      一般社団法人 日本血栓止血学会
    • Related Report
      2009 Annual Research Report
  • [Book] Recent Advances in Thrombosis and Hemostasis 2008.(Tanaka K, Davie EW eds.)(Snake Venoms and Other Toxic Components Affecting Thrombosis and Hemostasis.)2008

    • Author(s)
      Yamazaki Y, Morita T
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Recent Advances in Thrombosis and Hemostasis 20082008

    • Author(s)
      Yamazaki Y
    • Publisher
      Snake Venoms and Other Toxic Components Affecting Thrombosis and Hemostasis
    • Related Report
      2008 Annual Research Report
  • [Book] Xa阻害薬のすべて2007

    • Author(s)
      山崎 泰男
    • Publisher
      株式会社先端医学社
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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