| Project/Area Number |
19406012
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Oita University |
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Principal Investigator |
KAWAMOTO Fumihiko Oita University, 全学研究推進機構, 教授 (40115556)
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| Co-Investigator(Kenkyū-buntansha) |
KANBE Toshio 名古屋大学, 大学院・医学系研究科, 講師 (50093018)
OTSUKA Yasushi 大分大学, 医学部, 助教 (00244161)
新井 明治 産業医科大学, 医学部, 准教授 (30294432)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Meiji 香川大, 医学部, 准教授 (30294432)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 国際研究者交流 / インドネシア:ベトナム・カンボジア・タイ / 熱帯熱マラリア / 新型原虫 / 分子疫学調査 / ヒトマラリア原虫 / 遺伝子解析 / 生殖母体培養 / インドネシア:ベトナム / インドネシア:カンボジア:ベトナム / インドネシア:ベトナム:カンボジア:タイ |
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| Research Abstract |
During epidemiological study on malaria from 2005 to 2006, Plasmodium falciparum (Pf) with very thin morphology of gametocytes was found in 4 patients in Cambodia and Indonesia. Analysis of 18S ribosomal RNA gene indicated that they are belonging to the variant type of Pf detected from Vietnam, and they had the same sequence variation at the target region for molecular diagnosis. Therefore, in this study, we continued similar epidemiological study on the variant Pf whether the strange shape of gametocytes belongs to the variant Pf or not by analyzing the 18S ribosomal RNA gene. In addition, our study had another aim ; a trial to cultivate in vitro from gametocytes to sporozoites in the variant or ordinary Pf (also gametocytes of Plasmdium vivax ; Pv) at the field.
We surveyed malaria in Indonesia, Cambodia, Vietnam and Thailand from April 2007. In 2007, we found 2 patients infected with the variant Pf in Cambodia, but these bloods did not contain gametocytes. In addition, we detected 1 patient infected with Pv gametocytes, but trial of in vitro culture was failed. In 2008, we found 2 patients infected with gametocytes of the ordinary Pf, and tried in vitro culture of gametocytes. Furthermore, we detected 1 patient infected with Pv gametocytes in Cambodia. However, all these trials of in vitro culture were also failed. In 2009, no patient infected with the variant Pf was found in Indonesia and Vietnam. Therefore, it is still not clear whether morphology of gametocytes is different or not between the ordinary Pf and the variant Pf.
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