Etiological and epidemiological analysis of diarrheal diseases in tropical areas

• Project/Area Number: 19406014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Bacteriology (including Mycology)
• Research Institution: Nagasaki University
• Principal Investigator: ICHINOSE Yoshio Nagasaki University, 教授 (70176296)
• Co-Investigator(Kenkyū-buntansha): KANEKO Satoshi 長崎大学, 熱帯医学研究所, 教授 (00342907) ; TSUJI Takao 藤田保健衛生大学, 医学部, 教授 (60171998)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 下痢症 / コレラ / 疫学 / 病因学的分析 / DSS / マルチプレックスPCR / 病因論的分析 / HIV/AIDS / マルチプレルスPCR / 宿主抵抗性

Research Abstract

We have investigated to know the real situation of diarrheal disease outbreak in Kenya. We studies cholera outbreak started from South Nyanza in 2008 and diarrheal outbreak in Mandella near Somali border. We have also investigated causative agents among pediatric cases less than 5 years of age to evaluate the significance as diarrheagenic agents in the urbanized setting from 2008 to 2009. It reveals that Rota virus is an only major causative agent of diarrheal diseases and classical enteropathogens seemed to be no longer major diarrheagenic agents.

Research Products

• [Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity (2010)
  • Author(s): Akihiro Wada, Makoto Hasegawa, Pooi-Fong Wong, Emi Shirai, Nobuaki Shirai, Li-Jing Tan, Rafael Llanes, Hironobu Hojo, Eiki Yamasaki, Akitoyo Ichinose, Yoshio Ichinose, Masachika Senba
  • Journal Title: Glycobiology Vol, 28
  • NAID: 120006984983
  • Peer Reviewed
• [Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin(VacA)neutralizes its toxin activity (2010)
  • Author(s): Akihiro Wada, Makoto Hasegawa, Pooi-Fong Wong, Emi Shirai, Nobuaki Shirai, Li-Jing Tan, Rafael Llanes, Hironobu Hojo, Eiki Yamasaki, Akitoyo Ichinose, Yoshio Ichinose, Masachika Senba
  • Journal Title: Glycobiology 28
  • NAID: 120006984983
  • Peer Reviewed
• [Journal Article] Nucleotide Sequence Analysis of the Enterotoxigenic Escherichia coli Ent Plasmid (2009)
  • Author(s): Sadayuki Ochi, Tohru Shimizu, Kaori Ohtani, Yoshio Ichinose, Hideyuki Arimitsu, Kentaro Tsukamoto, Michio Kato, Takao Tsuji
  • Journal Title: DNA Research 16(5) Pages: 299-309
  • Peer Reviewed
• [Journal Article] Profile of the Nagasaki University Kenya Research Station and Activities (2007)
  • Author(s): Shimada M, Ichinose Y, Kaneko S, Minakawa N
  • Journal Title: Tropical Medicine and Health 35 Pages: 29-31
  • NAID: 10019734267
  • Peer Reviewed
• [Journal Article] Establishment of Bio-medical Laboratories in the Kenya Research Station and a Diarrheal Disease Research Project in Kenya (2007)
  • Author(s): Ichinose Y.
  • Journal Title: Tropical Medicine and Health 35 Pages: 33-36
  • NAID: 10019734271
  • Peer Reviewed
• [Journal Article] Profile of the Nagasaki University Kenya Research Station and Activities (2007)
  • Author(s): Shimada M, Ichinose Y, Kaneko S and Minakawa N
  • Journal Title: Tropical Medicine and Health 35 Pages: 29-31
  • NAID: 10019734267
  • Peer Reviewed
• [Journal Article] Estalishment of Bio-medical Laboratories in the Kenya Research Station and a Diarrheal Disease Research Project in Kenya (2007)
  • Author(s): Ichinose Y.
  • Journal Title: Tropical Medicine and Health 35 Pages: 33-36
  • Peer Reviewed
• [Presentation] Etiological investigation of diarrheal diseases in the suburbs of Nairobi (2011)
  • Author(s): Yoshio Ichinose, Martin Bundi, Angela Makum, Gaburiel Miringu, Mohamed Karama, Sora Suka, Mwajuma Abubakar, Victor Ager, Sam Kariuki, Sadayuki Ochi, Takao Tsuji, Sinao Inoue, Masaaki Shimada
  • Organizer: The Third International Meeting on Emerging Diseases and Surveillance (IMED 2011)
  • Place of Presentation: Vienna, Austria
• [Presentation] A Study on Effect on Education on Biosafety Technique in NUITM-KEMRI Laboratory, 2nd Annual Biological Safety Conference (2011)
  • Author(s): Bundi Martin, Miringu, Gabriel, Narita, Chika, Huka, Sora, Galata, Amina, Ager, Victor, Abubakar, Mwajuma, Maranga, Robert, Ashur, Salame, Shimada, Masaaki, Karama, Mohamed, Inoue, Shingo, Ichinose Yoshio
  • Organizer: 2nd Annual Biological Safety Conference
  • Place of Presentation: Acura, Gahna
• [Presentation] 2008年から2009年にかけてアフリカ・ケニア共和国キアンブ地域で検出されたヒトA群ロタウイルスのVP7、VP4、NSP4遺伝子型の分布 (2010)
  • Author(s): 荻野倫子、James Nyangao, Sora Suka, Robert Maranga, Martin Bundi, Victor Ager, Mwajuma Abubakar、Mohamed Karama, 嶋田雅暁、谷口孝喜、一瀬休生
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島、あわぎんホール
  • Year and Date: 2010-11-09
• [Presentation] Analysis of the transfer and plasmid maintenance regions of the enterotoxigenic Escherichia coli Ent plasmid pEntH10407 [P] (2010)
  • Author(s): Sadayuki Ochi, Tohru Shimizu, Kaori Ohtani, Yoshio Ichinose, Hideyuki Arimitsu, Kentaro Tsukamoto, Michio Kato, Toshiyasu Shimizu, Takao Tsuji.
  • Organizer: 日米医学協力研究会コレラ・細菌性腸管感染症専門部会
  • Place of Presentation: 京都、稲盛財団記念館
  • Year and Date: 2010-07-29
• [Presentation] Cholera outbreak (2010)
  • Author(s): Yoshio Ichinose
  • Organizer: MOPHS/JICA, JICA supported HSS for Primary Health Service Program
  • Place of Presentation: Kisumu Kenya
  • Year and Date: 2010-04-23
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの機能領域配列解析 (2010)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、Neri Paola、佐々木慶子、加藤道夫、一瀬休生、清水徹、辻孝雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜、パシフィコ横浜
• [Presentation] ガングリオシド樹脂を用いた細菌毒素の同定方法の検討 (2010)
  • Author(s): 和田昭裕、一瀬休生
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜、パシフィコ横浜
• [Presentation] A Study on Effect of Education on Biosafety Technique in NUITM-KEMRI Laboratory (2010)
  • Author(s): Chika Narita, Sora Suka, Amina Galata, Victor Ager, Mwajuma Abubakar, Gabriel Miringu, Martin Bundi, Masaaki Shimada, Mohamed Karama, Shingo Inoue, Yoshio Ichinose
  • Organizer: 1^<st> Annual Biological Safety Conference, African Biological Safety Association
  • Place of Presentation: KEMRI Training Centre Nairobi, Kenya
• [Presentation] Etiological investigation of diarrheal diseases in the suburbs of Nairobi, Kenya (2010)
  • Author(s): Yoshio Ichinose, Martin Bundi, Angela Makum, Gaburiel Miringu, Mohamed Karama, Sora Suka, Mwajuma Abubakar, Victor Ager, Sam Kariuki, Sadayuki Ochi, Takao Tsuji, Singo Inoue, Masaaki Shimada
  • Organizer: 第51回日本熱帯医学会大会
  • Place of Presentation: 仙台、仙台国際センター
• [Presentation] Cholera outbreak in Kenya (2010)
  • Author(s): Yoshio Ichinose
  • Organizer: Cholera Stakeholders Workshop at Sopa Lodge in Naivasha, Kenya, Ministry of Health, Kenya and AFRO, WHO
  • Place of Presentation: Naivasha, Kenya
• [Presentation] A Study on Effect of Education on Biosafety Technique in NUITM-KEMRI Laboratory (2010)
  • Author(s): Chika Narita, Sora Suka, Amina Galata, Victor Ager, Mwajuma, Abubakar, Gabriel Miringu, Robert Maranga
  • Organizer: 29^<th> World Congress of Biomedical Laboratory Sciences
  • Place of Presentation: KICC, Nairobi, Kenya
• [Presentation] Etiological investigation of diarrheal diseases in the suburbs of Nairobi, Kenya (2010)
  • Author(s): Yoshio Ichinose, Martin Bundi, Angela Makum, Gaburiel Miringu, Mohamed Karama, Sora Suka, Mwajuma Abubakar, Victor Ager, Sam Kariuki, Sadayuki Ochi, Takao Tsuji, Singo Inoue, Masaaki Shimada
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2010, Ministry of Education, Culture, Sports, Science and Technology (MEXT) "Japan Initiative for Global Research Network on Infectious Diseases (J-GRID)"
  • Place of Presentation: Hanoi, Vietnam
• [Presentation] A Study on Effect of Education on Biosafety Technique in NUITM-KEMRI Laboratory (2010)
  • Author(s): Chika Narita, Sora Suka, Amina Galata, VictorAger, Mwajuma Abubakar, Gabriel Miringu, Martin Bundi, Masaaki Shimada, Mohamed Karama, Shingo Inoue, Yoshio Ichinose
  • Organizer: 1^<st> Annual Biological Safety Conference, African Biological Safety Association,
  • Place of Presentation: KEMRI Training Centre Nairobi, Kenya
• [Presentation] 結核の世界の現状について (2009)
  • Author(s): 一瀬休生
  • Organizer: 五島保健所合同結核研修会,
  • Place of Presentation: 五島中央病院、長崎
  • Year and Date: 2009-11-24
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの接合伝達領域 (2009)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、佐々木慶子、加藤道夫、一瀬休生、清水徹、辻孝雄
  • Organizer: 第46回日本細菌学会中部支部総会
  • Place of Presentation: 名古屋、名城大学薬学部ライフサイエンスホール
• [Presentation] 西ケニアのコレラ流行とその起因菌の性状について (2009)
  • Author(s): 一瀬休生、江原雅彦、堀尾政博、荻野倫子、越智定幸、辻孝雄、嶋田雅暁、Mohamed Karama, Victor Okoth, Angella Makumi
  • Organizer: 第50回日本熱帯医学会総会
  • Place of Presentation: 沖縄、沖縄コンベンションセンター
• [Presentation] Water sources, treatment and storage at the household level in Suba district (2009)
  • Author(s): Sheru Waanyua, Mohamed, Karama, Ibrahim Kiche, James K' Opiyo, Yoshio Ichinose, Satoshi Kaneko
  • Organizer: The Association of public health officers (Kenya)
  • Place of Presentation: Kisumu, Kenya
• [Presentation] Characterization of Vibrio cholerae O1 strains isolated in northern Vietnam in 2007-2009 (2009)
  • Author(s): Nguyen Dong Tu, 岩見守、一瀬休生、江原雅彦
  • Organizer: 第62回日本細菌学会九州支部総会
  • Place of Presentation: 佐賀、佐賀大学医学部臨床大講義堂
• [Presentation] 西ケニアのコレラ流行とその起因菌の性状について (2009)
  • Author(s): 一瀬休生、江原雅彦、越智定幸、辻孝雄
  • Organizer: 第57回日本化学療法学会
  • Place of Presentation: 東京、ホテル日航東京
• [Presentation] ケニア共和国における黄熱ウイルス感染症のELISA法を用いた血清疫学とその他の蚊媒介性熱帯ウイルス感染症の血清学的解析 (2009)
  • Author(s): 久保亨、一瀬休生、森田公一
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京、京王プラザホテル
• [Presentation] Phenotypic and molecular typing of Vibrio cholerae 01 isolated in Western Kenya (2009)
  • Author(s): 一瀬休生, et al.
  • Organizer: 3^<rd> East African Health & Scientific Conference
  • Place of Presentation: Nairobi, Kenya
• [Presentation] 西ケニアで流行した01コレラ菌株の性状について (2009)
  • Author(s): 一瀬休生, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Characterization of Vibrio cholerae 01 strain isolated in Vietnam in 2007-2008 (2009)
  • Author(s): 江原雅彦, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Vibriophages in the northern province in Vietnam (2009)
  • Author(s): 呉呑都, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 西ケニアのコレラ流行とその起因菌の性状について (2009)
  • Author(s): 一瀬休生, 江原雅彦, 堀尾政博, 荻野倫子, 越智定幸, 辻孝雄, 嶋田雅暁, Mohamed Karama, Victor Okoth, Angella Makumi
  • Organizer: 第50回日本熱帯医学会総会
  • Place of Presentation: 沖縄
• [Presentation] Water sources, treatment and storage at the household level in Suba district. (2009)
  • Author(s): Sheru Waanyua, Mohamed, Karama, Ibrahim Kiche, James K'Opiyo, Yoshio Ichinose, Satoshi Kaneko.
  • Organizer: The Association of public health officers(Kenya)
  • Place of Presentation: Kisumu, Kenya
• [Presentation] Characterization of Vibrio cholerae 01 strains isolated in northern Vietnam in 2007-2009 (2009)
  • Author(s): Nguyen Dong Tu, 岩見守、一瀬休生、江原雅彦
  • Organizer: 第62回日本細菌学会九州支部総会
  • Place of Presentation: 佐賀、佐賀大学医学部臨床大講義堂
• [Presentation] 西ケニアのコレラ流行とその起因菌の性状について (2009)
  • Author(s): 一瀬休生、江原雅彦、堀尾政博、荻野倫子、越智定幸、辻孝雄、嶋田雅暁、Mohamed Karama, Victor Okoth, Angella Makumi
  • Organizer: 第50回日本熱帯医学套総会
  • Place of Presentation: 沖縄、沖縄コンベンションセンター
• [Presentation] Phenotypic and molecular typing of Yibrio cholerae 01 isolated in Western Kenya (2009)
  • Author(s): Yoshio Ichinose, et al.
  • Organizer: 3^<rd> East African Health & Scientific Conference
  • Place of Presentation: Nairobi, Kenya
• [Presentation] Vibriophages in the northern provinces in Vietnam (2008)
  • Author(s): Masahiko Ehara, et al.
  • Organizer: Joint Forum Hanoi, Vietnum
  • Place of Presentation: Hanoi, Vietnam
  • Year and Date: 2008-10-06
• [Presentation] Characterization of Vibrio cholerae 01 strains isolated in 2007-2008 in Northern Vietnam (2008)
  • Author(s): 江原雅彦, et al.
  • Organizer: US-Japan Cholera Panel
  • Place of Presentation: Fukuoka
• [Presentation] 長崎大学熱研内科での国際医療活動実施のための取組み-ケニア・ナイロビでの臨床医療活動の経験を通して- (2008)
  • Author(s): 氏家無限, 他
  • Organizer: 第23回日本国際保健医療学会大会
  • Place of Presentation: 東京
• [Presentation] 西ケニアで流行した01コレラ菌株の性状について (2008)
  • Author(s): 一瀬休生, 他
  • Organizer: 第61回日本細菌学会九州支部総会
  • Place of Presentation: 熊本
• [Presentation] 2007-2008年にベトナムで分離されたコレラ菌の性状について (2008)
  • Author(s): 江原雅彦, 他
  • Organizer: 第61回日本細菌学会九州支部総会
  • Place of Presentation: 熊本
• [Presentation] Phenotypic and molecular typing of Vibrio cholerae 01 isolated in Western Kenya (2008)
  • Author(s): 一瀬休生, et al.
  • Organizer: ICTM
  • Place of Presentation: Cheju, Korea
• [Presentation] Characterization of Vibrio cholerae 01 strains isolated in 2007-2008 in Northern Vietnam (2008)
  • Author(s): Masahiko Ehara, et al.
  • Organizer: US-Japan Cholera Panel
  • Place of Presentation: Osaka
• [Presentation] What is P3 Lab and why is it necessary (2007)
  • Author(s): Yoshio Ichinose
  • Organizer: Workshop for training P3 staff:Maintenance and Operation of P3 Laboratory System
  • Place of Presentation: Centre of Public Health Research,KEMRI,Nairobi,Kenya
  • Year and Date: 2007-11-20
• [Presentation] DSS site in Suba and method of data collection. (2007)
  • Author(s): Satoshi Kaneko, Mohamed Karama, Noboru Minakawa, Yoshio Ichinose, Rashid Agolla, Emmanuel Mushinzimana, James K'opiyo, Kazuhiko MOJI, Masaaki Shimada
  • Organizer: DSS site in Suba and method of data collection.
  • Place of Presentation: Embassy of Japan, Nairobi, Kenya
• [Presentation] DSS site in Suba and method of data collection (2007)
  • Author(s): Satoshi Kaneko, Mohamed Karama, Noboru Minakawa, Yoshio Ichinose, Rashid Agolla, Emmanuel Mushinzimana, JamesK'opiyo, Kazuhiko MOJI, Masaaki Shimada
  • Organizer: JSPS AA Science Platform Programme Seminar Report
  • Place of Presentation: Embassy of Japan,Nairobi,Kenya
• [Presentation] Vibriophages in the northern provinces in Vietnam
  • Author(s): 江原雅彦, et al.
  • Organizer: Joint Forum Hanoi, Vietnum, 6 Hanoi, Vietnam 6^<th> OCT 2008
• [Book] コレラ(今日の診断指針第6版)(総編集:金澤一郎・永井良三) (2010)
  • Author(s): 一瀬休生
  • Total Pages: 1290
  • Publisher: 医学書院
• [Book] アメーバ性大腸炎・他の腸管寄生原虫による疾患Amebic colitis and other intestinal protozoan infections(今日の消化器疾患治療指針第3版)(幕内雅敏、菅野健太郎、工藤正俊編) (2010)
  • Author(s): 一瀬休生
• [Book] 「コレラ」、今日の診断指針第6版(総編集 : 金澤一郎・永井良三) (2010)
  • Author(s): 一瀬休生
  • Total Pages: 1290
  • Publisher: 医学書院
• [Book] 「コレラ」、今日の診断指針第6版、総編集 : 金澤一郎・永井良三、 (2010)
  • Author(s): 一瀬休生
  • Total Pages: 1
  • Publisher: 医学書院
• [Book] 「アメーバ性大腸炎・他の腸管寄生原虫による疾患Amebic colitis and other intestinal protozoan infections」今日の消化器疾患治療指針第3版、幕内雅敏、菅野健太郎、工藤正俊編 (2010)
  • Author(s): 一瀬休生
  • Total Pages: 1
  • Publisher: 医学書院
• [Book] 新興・再興感染症とは(国際貢献 -医療に携わる人たちのために-)(大塚吉兵衛編) (2008)
  • Author(s): 一瀬休生
  • Publisher: HYOURON
• [Book] 新興・再興感染症とは?国際貢献 -医療に携わる人たちのために- 大塚吉兵衛編 (2008)
  • Author(s): 一瀬 休生
  • Publisher: HYOURON
• [Patent(Industrial Property Rights)] 病原性グラム陰性細菌由来脂質小胞の検出方法および検出システム (2010)
  • Inventor(s): 長谷川慎、和田昭裕、一瀬休生、白井伸明、武居修
  • Industrial Property Rights Holder: 長浜バイオ大学(50%)、長崎大学(15%)、滋賀県工業技術総合センター(15%)、(株)ライフテック(20%)
  • Industrial Property Number: 2010-035441
  • Filing Date: 2010
• [Patent(Industrial Property Rights)] 病原性グラム陰性細菌由来脂質小胞の検出方法および検出システム (2010)
  • Inventor(s): 長谷川慎, 和田昭裕, 一瀬休生, 白井伸明, 武居修
  • Industrial Property Rights Holder: 長浜バイオ大学 (50%)、長崎大学 (15%)、滋賀県工業技術総合センター (15%)、(株) ライフテック (20%)
  • Industrial Property Number: 2010-035441
  • Filing Date: 2010