Molecular epidemiology study on carcinogenic mechanism of Epstein-Barr-associated nasopharyngeal carcinoma and modification by environmental factors in southern China
| Project/Area Number |
19406021
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hygiene
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| Research Institution | Mie University |
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Principal Investigator |
MURATA Mariko Mie University, 大学院・医学系研究科, 教授 (10171141)
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| Co-Investigator(Kenkyū-buntansha) |
OIKAWA Shinji 三重大学, 大学院・医学系研究科, 准教授 (10277006)
HIRAKU Yusuke 三重大学, 医学系研究科, 講師 (30324510)
MA Nei 鈴鹿医療科学大学, 保健衛生学部, 教授 (30263015)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 中国南部 / Epstein-Barrウィルス / 上咽頭癌 / 8-ニトログアニン / 炎症 / 8-ハイドロキシデオキシグアノシン / DNAメチル化 / Ras-related associated with diabetes(RRAD) / がん抑制遺伝子 / エピジェネティック異常 / ubiquitin carboxyl-terminal hydrolase L1(UCHL1) / メチル化特異的PCR / 中国南部地域 / 感染・炎症関連発がん / 誘導型一酸化窒素合成酵素 / 8-oxodG / バイオマーカー |
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| Research Abstract |
Epstein-Barr virus-infected nasopharyngeal carcinoma (NPC) is an important health problem in southern China. We obtained serum and biopsy specimens of nasopharyngeal tissues from NPC patients in southern China. We performed double immunofluorescent staining to examine the formation of nitrative and oxidative DNA lesions (8-nitroguanine, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG)) in these specimens. DNA lesions were observed in cancer cells and inflammatory cells of NPC patients. Intensive immunoreactivity of iNOS was detected in the cytoplasm of cancer cells. Nuclear accumulation of EGFR and STAT3 activation by IL-6 may play the key role in iNOS expression and resultant DNA damage, leading to EBV-mediated NPC. The serum level of 8-oxodG in the NPC patients was significantly higher than that in the control. Promoter hypermethylation of Ras-related associated with diabetes (RRAD) was detected in all six NPC cell lines and most of primary NPC tissues. These findings may provide a strategy for preventing the development of NPC.
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Report
(4 results)
Research Products
(71 results)
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[Journal Article] Urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine in patients with parasite infection and the effect of anti-parasitic drug in relation to cholangiocarcinogenesis.2008
Author(s)
Thanan R., Murata M, Pinlaor S, Sithithaworn P, Khuntikeo N, Tangkanakul W, Hiraku Y, Oikawa S, Yongvanit P, Kawanishi S.
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Journal Title
Cancer Epidemiol Biomarkers Prev 17(3)
Pages: 518-24
Related Report
Peer Reviewed
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