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Optical imaging of the depolarization wave in the embryonic CNS -Mechanisms regulating the switching from primordial activity to the respiratory rhythm-

Research Project

Project/Area Number 19500268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKanto Gakuin University

Principal Investigator

MOMOSE-SATO Yoko  Kanto Gakuin University, 人間環境学部, 教授 (70251501)

Co-Investigator(Kenkyū-buntansha) 佐藤 勝重  東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (80291342)
Co-Investigator(Renkei-kenkyūsha) SATO Katsushige  東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (80291342)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsdepolarization wave / 自発活動 / 中枢神経系 / 発生 / 光学計測 / 膜電位感受性色素 / 鶏胚 / gap junction / 脳幹 / 神経回路網 / ラット胚 / 自発性興奮 / 呼吸中枢
Research Abstract

発生初期の中枢神経系で見られる広範囲伝播脱分極波から、成体呼吸中枢リズム活動へのスイッチングがどのようなメカニズムで起こるのかを明らかにするために、光学イメージングによる解析を行った。発生の早い時期にはobexから腰髄までの多数の領域から起こっていた自発性脱分極波は、発生の後期にはほとんどが腰仙髄から起こり、その後の広範囲伝播性の消失が契機となって呼吸中枢の自発的リズム活動が発現してくるものと考えられる。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (50 results)

All 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (27 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Origin of the earliest correlated neuronal activity in the chick embryo revealed by optical imaging with voltage-sensitive dyes2009

    • Author(s)
      Momose-Sato,Y., Mochida, H. and Kinoshita, M
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 1-13

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo2009

    • Author(s)
      Mochida, H., Sato, K. and Momose-Sato, Y.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 14-30

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Origin of the earliest correlated neuronal activity in the chick embryo revealed by optical imaging with voltage-sensitive dyes.2009

    • Author(s)
      Momose-Sato, Y, et al.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 1-13

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo.2009

    • Author(s)
      Mochida, H, et al.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 14-30

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical analysis of neural circuit formation in the embryonic brain2008

    • Author(s)
      Sato. K. and Momose-Sato, Y.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 35

      Pages: 706-713

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos2008

    • Author(s)
      Glover, J. C., Sato, K. and Momose-Sato, Y.
    • Journal Title

      Developmental Neurobiology 68

      Pages: 804-816

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical imaging analysis of neural circuit formation in the embryonic brain.2008

    • Author(s)
      Sato. K., Momose-Sato, Y.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 35

      Pages: 706-713

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos.2008

    • Author(s)
      Glover, J. C, et al.
    • Journal Title

      Developmental Neurobiology 68

      Pages: 804-816

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos.2008

    • Author(s)
      Glover, et. al.
    • Journal Title

      Developmental Neurobiology 68

      Pages: 804-816

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical analysis of neural circuit formation in the embryonic brain.2008

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 35

      Pages: 706-713

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS2007

    • Author(s)
      Momose-Sato, Y., Sato, K. and Kinoshita, M.
    • Journal Title

      European Journal of Neuroscience 25

      Pages: 929-944

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway2007

    • Author(s)
      Sato, K., Kinoshita, M. and Momose-Sato, Y.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M. and Sato, K.
    • Journal Title

      Neuroscience 148

      Pages: 140-150

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M. and Sato, K.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in the chick brainstem as revealed by voltage-sensitive dye recording2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M. and Sato, K.
    • Journal Title

      Neuroscience Letters 423

      Pages: 138-142

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      European Journal of Neuroscience 25

      Pages: 929-944

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience 148

      Pages: 140-150

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., et. al.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in the chick brainstem as revealed by voltage-sensitive dye recording.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience Letters 423

      Pages: 138-142

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GABA-induced intracellular Ca2+ elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki. T., et. al.
    • Journal Title

      Brain Research 1142

      Pages: 19-27

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Functional organization of the glossopharyngeal and vagus nerve-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes2008

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo2008

    • Author(s)
      Momose-Sato, Y., Mochida, H. and Sato, K.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Neuronal excitability to generate spontaneous activity in the medulla and spinal cord of the chick embryo2008

    • Author(s)
      Kinoshita, M., Momose-Sato, Y. and Sato, K.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Regional gradient of neuronal excitability to generate spontaneous activity in the hindbrain of the chick embryo2008

    • Author(s)
      Kinoshita, M., Momose-Sato, Y. and Sato, K.
    • Organizer
      6th FENS Forum of European Neuroscience
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹における迷走神経求心線維の機能発達過程の光学的解析2008

    • Author(s)
      佐藤勝重、木下雅恵, 佐藤容子
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 光学的イメージングによる鶏胚中枢神経系における脱分極波のoriginの同定2008

    • Author(s)
      佐藤容子、持田啓、木下雅恵、佐藤勝重
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚後脳における神経興奮性の部位差2008

    • Author(s)
      木下雅恵, 佐藤容子、佐藤勝重
    • Organizer
      第3 回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹迷走神経回路網の機能発達過程の光学的解析2008

    • Author(s)
      佐藤勝重、木下雅恵, 佐藤容子
    • Organizer
      第85回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚中枢神経系における脱分極波のoriginの同定 : 光学的イメージングによる解析2008

    • Author(s)
      佐藤容子、持田啓、木下雅恵、佐藤勝重
    • Organizer
      第85回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo.2008

    • Author(s)
      Momose-Sato, Y, et al.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Functional organization of the glossopharyngeal and vagus nerve-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes.2008

    • Author(s)
      Sato, K., Momose-Sato, Y.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem: optical imaging with voltage-sensitive dyes.2008

    • Author(s)
      Sato, et. al.
    • Organizer
      日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Origin of the depolarization wave at an initial stage of wave expression revealed by optical imaging.2008

    • Author(s)
      Momose-Sato, et. al.
    • Organizer
      日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording2007

    • Author(s)
      Sato, K., Kinoshita, M. and Momose-Sato, Y.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] The origin of the depolarization wave at an early phase of the chick development revealed by optical imaging with a voltage-sensitive dye2007

    • Author(s)
      Momose-Sato, Y., Mochida, H., Kinoshita, M. and Sato, K.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Regulation of spontaneous rhythmic activities in the caudal medulla by the spinal cord in the chick embryo2007

    • Author(s)
      Kinoshita, M., Momose-Sato, Y. and Sato, K.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹聴覚神経回路網の機能発達過程の光学的解析2007

    • Author(s)
      佐藤勝重、Glover J. C., 佐藤容子
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット胚中枢神経系における自発興奮波の時空間パターンとoriginの同定 : 光学的イメージングによる解析2007

    • Author(s)
      佐藤容子、佐藤勝重、木下雅恵
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット皮質線条体における膜電位感受性色素による神経伝達イメージング2007

    • Author(s)
      稲次基希、佐藤勝重、成相直、百瀬-佐藤容子、大野喜久郎
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Depolarization waves in the embryonic CNS visualized with voltage-sensitive dye imaging2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M. and Sato, K.
    • Organizer
      7th IBRO World Congress of Neuroscience
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2008 Final Research Report
  • [Presentation] Intraoperative intrinsic optical imaging in brain tumor surgery2007

    • Author(s)
      Inaji, M., Nariai, T., Sato, K., Momose-Sato, Y., Maehara, T. and Ohno, K.
    • Organizer
      6th Congress of the Asian Society for Stereotactic, Functional and Computer Assisted Neurosurgery
    • Place of Presentation
      Fujiyoshida, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording.2007

    • Author(s)
      Sato, et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] The origin of the depolarization wave at an early phase of the chick development revealed by optical imaging with a voltage-sensitive dye.2007

    • Author(s)
      Momose-Sato, et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Regulation of spontaneous rhythmic activities in the caudalmedulla by the spinal cord in the chick embryo.2007

    • Author(s)
      Kinoshita, et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Functional development of synaptic connections in the chick auditory system: Optical imaging with a voltage-sensitive dye.2007

    • Author(s)
      Sato, et. al.
    • Organizer
      日本神経科学会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Origins of the spontaneous depolarization wave in the embryonic rat CNS revealed by optical imaging with a voltage-sensitive dye.2007

    • Author(s)
      Momose-Sato, et. al.
    • Organizer
      日本神経科学会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Neuronal activity imaging in the rat cortico-striatum pathway using voltage-sensitive dye.2007

    • Author(s)
      Inaji, et. al.
    • Organizer
      日本神経科学会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Book] Neural Pathways: New Research2008

    • Author(s)
      Maekawa, F., et. al.
    • Publisher
      Nova Science Publishers (in press)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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