Identification of neural stem cell survival factor

• Project/Area Number: 19500274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: SHIMAZAKI Takuya Keio University, 医学部, 講師 (00324749)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経幹細胞 / PA-6 / 生存因子 / ES 細胞 / クローニング / ES細胞

Research Abstract

近年、様々な研究によって神経幹細胞の維持・分化機構等の解明が進んでいるが、神経幹細胞の維持における生存機構についてはあまりよく分かっておらず、いわゆる生存因子とされるものも同定されていなかった。そこで本研究では、ストローマ細胞の一種であるPA-6 の培養上澄がマウスES 細胞由来および胎仔脳由来神経幹細胞の低密度培養下でのneurosphere 形成を促進する活性を持つことを見出し、その活性分子の少なくとも1つと考えられるものを精製・同定した。

Research Products

• [Journal Article] Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders : properties and tumorigenicity after long-term in vitro maintenance (2009)
  • Author(s): Ogawa D, Okada Y, Nakamura M, Kanemura Y, Okano HJ, Matsuzaki Y, Shimazaki T, Ito M, Ikeda E, Tamiya T, Nagao S, Okano H
  • Journal Title: J Neuroci Res 87 Pages: 307-317
  • Peer Reviewed
• [Journal Article] Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders : properties and tumorigenicity after long-term in vitro maintenance. (2009)
  • Author(s): Ogawa D, et al.
  • Journal Title: J Neuroci Res 87 Pages: 307-317
  • Peer Reviewed
• [Journal Article] Requirement for COUP-TFI and II in the temporal specification of neural stem cells in central nervous system development (2008)
  • Author(s): Naka H, Nakamura S, Shimazaki T, Okano H
  • Journal Title: Nature Neurosci 11 Pages: 1014-1023
  • Peer Reviewed
• [Journal Article] Spatio-temporal recapitulation of central nervous system development by ES cell-derived neural stem/progenitor cells (2008)
  • Author(s): Okada Y, Matsumoto A, Shimazaki T, Enoki R, Koizumi A, Ishii S, Itoyama Y, Sobue G, Okano H
  • Journal Title: Stem Cells 26 Pages: 3086-3098
  • Peer Reviewed
• [Journal Article] Requirement for COUP-TFI and II in the temporal specification of neural stem cells in central nervous system development. (2008)
  • Author(s): Naka H, et al.
  • Journal Title: Nature Neurosci 11 Pages: 1014-1023
  • Peer Reviewed
• [Journal Article] Spatio-temporal recapitulation of central nervous system development by ES cell-derived neural stem/progenitor cells. (2008)
  • Author(s): Okada Y, et al.
  • Journal Title: Stem Cells 26 Pages: 3086-3098
  • Peer Reviewed
• [Journal Article] Simian fetal brain progenitor cells for studying viral neuropathogenesis (2007)
  • Author(s): Iwata N, Yoshida H, Tobiume M, Ono F, Shimazaki T, Sata T, Nakajima N
  • Journal Title: J Neurovirol 13 Pages: 11-22
  • Peer Reviewed
• [Journal Article] Epidermal growth factor signaling mediated by grb2 associated binder1 is required for the spatiotemporally regulated proliferation of olig2-expressing progenitors in the embryonic spinal cord (2007)
  • Author(s): Hayakawa-Yano Y, Nishida K, Fukami S, Gotoh Y, Hirano T, Nakagawa T, Shimazaki T, Okano H
  • Journal Title: Stem Cells 25 Pages: 1410-1422
  • Peer Reviewed
• [Journal Article] Leptin suppresses ghrelin-induced activation of neuropeptide Yneurons in the arcuate nucleus via phosphatidylinositol 3-kinase-and phosphodiesterase 3-mediated pathway (2007)
  • Author(s): Kohno D, Nakata M, Maekawa F, Fujiwara K, Maejima Y, Kuramochi M, Shimazaki T, Okano H, Onaka T, Yada T.
  • Journal Title: Endocrinology 148 Pages: 2251-2263
  • Peer Reviewed
• [Journal Article] Simian fetal brain progenitor cells for studying viral neuropathogenesis (2007)
  • Author(s): Iwata N, Yoshida H, Tobiume M, Ono F, Shimazaki T, Sata T, Nakajima N.
  • Journal Title: J Neurovirol 13 Pages: 11-22
  • Peer Reviewed
• [Journal Article] Epidermal growth factor signaling mediated by grb2 associated binderl is required for the spatiotemporally rcgulated proliferration of olig2-expressing progenitors in the emblyonic spinal cord (2007)
  • Author(s): Hayakawa-Yano Y, Nishida K, Fukami S, Gotoh Y, Hirano T, Nakagawa T, Shimazaki T, Okano H.
  • Journal Title: Stem Cells 25 Pages: 1410-1422
  • Peer Reviewed
• [Journal Article] Leptin suppresses ghrelin-induced activation of neuropeptide Y neurons in the arcuate nucle us via phosphatidylinositol 3-kinase- and phosphodiesterase 3-mediated pathway. (2007)
  • Author(s): Kohno D, Nakata M, Maekawa F, Fujiw ara K, Maejima Y, Kuramochi M, Shimazaki T, Onaka T, Yada T.
  • Journal Title: Endocrinology 148 Pages: 2251-2263
  • Peer Reviewed
• [Presentation] Stromal cell-secreted factors promote the survival of ES cell-derived early neural stem/progenitor cells via the activation of MAPK and PI3K-Akt pathways (2008)
  • Author(s): Seiji Ishii, et.al.
  • Organizer: 第31回日本分子生物学会年会,第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Stromal cell-secreted factors promote the survival of ES cell-derived early neural stem/progenitor cells via the activation of MAPK and PI3K-Akt pathways (2008)
  • Author(s): Seiji Ishii, et al.
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] ストローマ細胞が分泌する液性因子はMAPKおよびPI3K-Akt 経路の活性化を介してES 細胞由来の発生早期神経幹細胞/前駆細胞の生存を促進する (2008)
  • Author(s): 石井聖二, 他
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] ストローマ細胞が分泌する液性因子はMAPKおよびPI3K-Akt経路の活性化を介してES細胞由来の発生 (2008)
  • Author(s): 石井聖二, 他
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Remarks]
  • URL: http://www.okano-lab.com/