Analysis of Regenerative Process after Peripheral Nerve Injury

• Project/Area Number: 19500276
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: SHIBATA Shinsuke Keio University, 医学部, 助教 (70407089)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 末梢神経 / 再生医学 / Musashi / RNA 結合タンパク質 / RNA結合タンパク質

Research Abstract

RNA 結合蛋白質Musashi2(Msi2)の遺伝子欠損マウスは、知覚神経障害をきたし、その原因が標的遺伝子の一つである神経軸索進展促進因子Pleiotrophin(Ptn)の減少によることが明らかになった。また末梢神経損傷後の回復過程で、Msi2 遺伝子欠損マウスは標的のPtn の発現上昇が少なく、機能回復が遅延することが分かった。末梢神経障害後に上昇するMsi2 はPtn の発現を促進し、触覚や運動機能回復のために重要な役割を担っていることが示された。

Research Products

• [Journal Article] Visualization of peripheral nerve degeneration and regeneration : Monitoring with diffusion tensor tractography (2009)
  • Author(s): Takagi T, Nakamura M, Yamada M, Hikishima K, Momoshima S, Fujiyoshi K, Shibata S, Okano HJ, Toyama Y, Okano H
  • Journal Title: Neuroimage 44(3) Pages: 884-892
  • Peer Reviewed
• [Journal Article] Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors (2009)
  • Author(s): Susaki K, Kaneko J, Yamano Y, Nakamura K, Inami W, Yoshikawa T, Ozawa Y, Shibata S, Matsuzaki O, Okano H, Chiba C
  • Journal Title: Experimental Eye Research 88(3) Pages: 347-355
  • Peer Reviewed
• [Journal Article] Visualization of peripheral nerve degeneration and regeneration : Monitoring with diffusion tensor tractography. (2009)
  • Author(s): Takagi T, Nakamura M, Yamada M, Hikishima K, Momoshima s, Fujiyoshi K, Shibata S, Okano HJ, Toyama Y, Okano H.
  • Journal Title: Neuroimage 44 Pages: 884-892
  • Peer Reviewed
• [Journal Article] Spinal cord injury : emerging beneficial role of reactive astrocytes' migration (2008)
  • Author(s): Renault-Mihara F, Okada S, Shibata S, Nakamura M, Toyama Y, Okano H
  • Journal Title: Int J Biochem Cell Biol 40(9) Pages: 1649-1653
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad (2008)
  • Author(s): Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H
  • Journal Title: Cell Stem Cell 2(4) Pages: 392-403
  • Peer Reviewed
• [Journal Article] Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors (2008)
  • Author(s): Susaki K, Kaneko J, Yamano Y, Nakamura K, Inami w, Yoshikawa T, Ozawa Y, Shibata S, Matsuzaki O, Okano H, Chiba C
  • Journal Title: Experimental Eye Research 88 Pages: 347-355
  • Peer Reviewed
• [Journal Article] spinal cord injury : emerging beneficial role of reactive astrocytes' migration. (2008)
  • Author(s): Renault-Mihara F, Okada s, Shibata S, Nakamura M, Toyama Y, Okano H.
  • Journal Title: Int J Biochem Cell Biol. 40 Pages: 1649-1653
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad. (2008)
  • Author(s): Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa s, Okada Y, Mabuchi Y, Katoh H, Okada s, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H.
  • Journal Title: Cell Stem Cell. 2 Pages: 392-403
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor promotes endogenous repair and functional recovery after spinal cord injury (2007)
  • Author(s): Kitamuta K., Iwanami A., Nakamura M., Yamane J., Kota W., Suzuki Y., Miyakawa D., Shibata S., Funakoshi H., Miyatakae S., Coffin R., Nakamura T., Toyama Y., Okano H.
  • Journal Title: J. Neurosci Res. 85(11) Pages: 2332-2342
  • Peer Reviewed
• [Journal Article] "Hepatocyte growth factor promotes endogenous repair and functional recovery after spinal cord injury." (2007)
  • Author(s): Kitamuta K., Iwanami A., Nakamur a M., Yamane J., Kota W., Suzuki Y., Miyakawa D., Shibata S., Funakoshi H., Miyatakae s., Coffin R., Nakamura t., Toyama Y., Okano H.
  • Journal Title: Journal of neuroscience research 85(11) Pages: 2332-2342
  • Peer Reviewed
• [Presentation] Analysis of RNA binding protein Musashi-2 in mammalian nervous system development. (2008)
  • Author(s): SHIBATA S, SAKAKIBARASI, OKANO HJ, OKANO H
  • Organizer: Cold Spring Harbor Laboratory Meeting "Axon Guidance, Synaptogenesis & Neural Plasticity"
  • Place of Presentation: New York, USA
  • Year and Date: 2008-09-13
• [Presentation] Analysis of RNA binding protein Musashi-2 in mammalian nervous system development (2008)
  • Author(s): SHIBATA S ; SAKAKIBARA SI ; OKANO HJ ; OKANO H
  • Organizer: Cold Spring Harbor Laboratory Meeting "Axon Guidance, Synaptogenesis & Neural Plasticity"(September 13^<th>Poster presentation)
  • Place of Presentation: New York, USA
• [Presentation] Analysis of RNA binding protein Musashi2 in mammalian nervous system development (2008)
  • Author(s): SHIBATA S ; SAKAKIBARA SI ; OKANO HJ ; OKANO H
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京都千代田区(ポスター発表)
• [Presentation] In vivo and in vitro analysis of Musashi family in mammalian CNS and PNS development (2007)
  • Author(s): Shinsuke SHIBATA;Shin-ichi SAKAKIBARA;Hirotaka J. OKANO;Hideyuki OKANO
  • Organizer: Society for Neuroscience 2007
  • Place of Presentation: San Diego CA, USA
  • Year and Date: 2007-11-05
• [Presentation] In vivo and in vitro analysis of Musashi family in mammalian CNS and PNS development and regeneration (2007)
  • Author(s): Shinsuke SHIBATA, Shin-ichi SAKAKIBARA, Takao IMAI, Hirotaka J. OKANO, Hideyuki OKANO
  • Organizer: 第30回日本神経科学大会・第50回日本神経化学会大会・第17回日本神経回路学会大会合同年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2007-09-11
• [Presentation] In vivo and in vitro analysis of Musashi family in mammalian CNS and PNS development (2007)
  • Author(s): SHIBATA S ; SAKAKIBARA SI ; OKANO HJ ; OKANO H
  • Organizer: Society for Neuroscience 2007(November 5^<th> Poster presentation)
  • Place of Presentation: San Diego CA, USA
• [Presentation] In vivo and in vitro analysis of Musashi family in mammalian CNS and PNS development and regeneration (2007)
  • Author(s): SHIBATA S ; SAKAKIBARA SI ; IMAI T ; OKANO HJ ; OKANO H
  • Organizer: 第30回日本神経科学大会・第50回日本神経化学会大会・第17回日本神経回路学会大会合同年会
  • Place of Presentation: 神奈川県横浜市(ワークショップにて口頭発表)