Functional analysis of Microglia derived fractalkine and curative effect to injured neuron

• Project/Area Number: 19500303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Jikei University School of Medicine (2008); Osaka City University (2007)
• Principal Investigator: MAEDA Mitsuyo Jikei University School of Medicine, 医学部, 講師 (40122080)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: ミクログリア / フラクタルカイン / 神経損傷 / ケモカイン / CX3CR1 / CNS / 神経損害

Research Abstract

傷害時ミクログリアに発現誘導されるCX3CR1の機能を検索するため、CX3CR1(-/-)マウスを用い、実験的神経因性疼痛モデルを作製し免疫組織学的に検索した。ミクログリアはワイルド(WT)と同様に脊髄後角第2層に浸潤した。WT群では有髄線維のミエリン接着貪食像が観察されたが、KOではその割合は減少した。CX3CR1はミクログリアの浸潤には関与せず、浸潤後の有髄軸索ミエリンの接着取り込みの調整への関与が示唆された。

Research Products

• [Journal Article] New potential therapy for orthotopic bladder carcinomaby combining HVJ envelope with doxorubicin (2008)
  • Author(s): Kawano, H., Komaba, S., Yamasaki, T., Maeda M., Kimura, Y., Maeda, A., Kaneda, Y
  • Journal Title: Cancer Chemotheraphy Pharmacology 61(6) Pages: 973-978
• [Journal Article] Expression of pancreatitis-associated proteins in urothelium and urinary afferent neurons following cyclophosphamide-induced cystitis (2008)
  • Author(s): Takahara, Y, Suzuki A, Maeda M, Kawashima H, Nakatani T, Kiyama H
  • Journal Title: J Urolo 179(4) Pages: 1603-1609
• [Journal Article] New potential therapy for orthotopic bladder carcinomaby combining HVJ envelope with doxorubicin. (2008)
  • Author(s): Kawano. H.
  • Journal Title: Cancer Chemotheraphy Pharmacology 61 Pages: 973-978
  • Peer Reviewed
• [Journal Article] Expression of pancreatitis-associated proteins in urothelium and urinary afferent neurons following cyclophosphamide-induced cystitis. (2008)
  • Author(s): Takahara. Y
  • Journal Title: J Urolo 179 Pages: 1603-1609
  • Peer Reviewed
• [Journal Article] Erythropoietin contributes to implantation : Ectopic hemoglobin synthesis in decidual cells of mice (2007)
  • Author(s): Yasuda Y, Sasaki T, Takagawa M, Maeda M, Yasuda M, Atsumi T, Fujita Y, Fujita H
  • Journal Title: Congenital Anomalies 47 Pages: 22-33
  • NAID: 10027386130
• [Journal Article] Transient suppression of the vesicular acetylcholine transporter in urinary bladder pathways following spinal cord injury (2007)
  • Author(s): Takahara Y, Maeda M, Nakatani T, Kiyama H
  • Journal Title: Brain res 1137 Pages: 20-28
• [Journal Article] Vlgr1 is required for proper stereocilia maturation of cochlear hair cells (2007)
  • Author(s): Yagi H, Tokano H, Maeda M, Takabayashi T, Nagano T, Kiyama H, Fujieda S, Kitamura K, Sato M
  • Journal Title: Genes Cells 12 Pages: 235-250
• [Presentation] Interaction between axotomized hypoglossal motor neuron and activated microglia in axotomy-induced neuronal death of the adult mouse (2007)
  • Author(s): Maeda M
  • Organizer: 第112回解剖学会総会全国学術集会
  • Place of Presentation: 京都
  • Year and Date: 2007-03-21