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Simplified method for the detection of prion protein oligomers

Research Project

Project/Area Number 19500309
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyushu University

Principal Investigator

SASAKI Kensuke  Kyushu University, 医学研究院, 助教 (80380616)

Co-Investigator(Kenkyū-buntansha) 岩城 徹  九州大学, 医学研究院, 教授 (40221098)
Co-Investigator(Renkei-kenkyūsha) IWAKI Toru  九州大学, 医学研究院, 教授 (40221098)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsプリオン蛋白 / オリゴマー / 界面活性剤 / ゲル濾過 / 遠心カラム / プロテアーゼ
Research Abstract

遠心カラムを利用した簡便なゲル濾過法により、異常プリオン蛋白オリゴマーと正常型モノマーを分離する方法を開発した。ヒトのプリオン病であるクロイツフェルト・ヤコブ病の剖検脳標本と、モデルマウスを用いた経時的解析で、オリゴマーの増加が病理変化の強さや病期の進行と相関することが示された。異常オリゴマーは従来のプロテアーゼ抵抗性という指標に対して中間的な性質を示した。この検査法は病態解明のための新たな解析方法として有用である。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (10 results)

  • [Journal Article] Clinicopathological outline of dementia with Lewy bodies applying the revised criteria : the Hisayama study2008

    • Author(s)
      Fujimi K, Sasaki K, Noda K, Wakisaka Y, Tanizaki Y, Matsui Y, Sekita A, Iida M, Kiyohara Y, Kanba S, Iwaki T.
    • Journal Title

      Brain Pathol 18 (3)

      Pages: 317-325

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological outline of dementia with Lewy bodies applying therevised criteria : the Hisayama study.2008

    • Author(s)
      Fujimi K., et al.
    • Journal Title

      Brain Pathol 18

      Pages: 317-325

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 退行期うつ病で長期入院中に発症した孤発性Creutzfeldt-Jakob病2007

    • Author(s)
      前川敏彦、佐々木健介、安岡克倫、田北昌史、門司晃、神庭重信
    • Journal Title

      精神科治療学 22(11)

      Pages: 1313-1318

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] プリオン病の病理解剖と標本作製の留意点2007

    • Author(s)
      佐々木健介、岩城徹
    • Journal Title

      病理と臨床 25

      Pages: 1124-1130

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Altered Expression of COX-2 in Subdivisions of the Hippocampus during Aging and in Alzheimer's Disease : The Hisayama Study2007

    • Author(s)
      Fujimi K, Noda K, Sasaki K, Wakisaka Y, Tanizaki Y, Iida M, Kiyohara Y, Kanba S, Iwaki T.
    • Journal Title

      Dement Geriatr Cogn Disord 23(6)

      Pages: 323-331

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Altered expression of COX-2 in subdivisions of the hippocampus during aging and in Alzheimer's disease:the Hisayama study2007

    • Author(s)
      Fujimi K., et. al.
    • Journal Title

      Dement. Geriatr. Cogn. Disord. 23

      Pages: 423-431

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プリオン病の病理解剖と標本作製の留意点2007

    • Author(s)
      佐々木 健介, ら
    • Journal Title

      病理と臨床 25

      Pages: 1124-1130

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Development of oligomeric prion-protein aggregate s in a mouse model of prion disease

    • Author(s)
      Sasaki K, Minaki H, Iwaki T.
    • Journal Title

      J Pathol (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prion protein oligomers in Creutzfeldt-Jakob disease detected by gelfiltration centrifuge columns

    • Author(s)
      Minaki H, Sasaki K, Honda H, Iwaki T.
    • Journal Title

      Neuropathology (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prion protein oligomers in Creutzfeldt-Jakob disease detected bygel-filtration centrifuge columns.

    • Author(s)
      Minaki H., et al.
    • Journal Title

      Neuropathology in press

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] プリオン病モデルマウスおける重合プリオン蛋白形成の経時的解析2008

    • Author(s)
      佐々木健介ら
    • Organizer
      第49回日本神経病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] プリオン病の病理形態学的および蛋白生化学的定量解析の試み2008

    • Author(s)
      本田裕之ら
    • Organizer
      第49回日本神経病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] プリオン蛋白オリゴマーの簡便な検出法2008

    • Author(s)
      皆木晴彦ら
    • Organizer
      第49回日本神経病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 耐糖能異常とアルツハイマー病の病理学的関連2008

    • Author(s)
      松崎尊信ら
    • Organizer
      第49回日本神経病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] プリオン病モデルマウスにおける重合プリオン蛋白形成の経時的解析2008

    • Author(s)
      佐々木健介、皆木晴彦、岩城徹
    • Organizer
      第49回日本神経病理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] プリオン蛋白オリゴマーの簡便な検出法2008

    • Author(s)
      皆木晴彦、佐々木健介、岩城徹
    • Organizer
      第49回日本神経病理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] プリオン病の病理形態学的および蛋白生化学的定量解析の試み2008

    • Author(s)
      本田裕之、皆木晴彦、柴野智子、佐々木健介、岩城徹
    • Organizer
      第49回日本神経病理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Time course of the development of PrP aggregates in a mouse model of prion disease2007

    • Author(s)
      Sasaki K, Minaki H, Iwaki T.
    • Organizer
      Prion2007
    • Place of Presentation
      Edinburgh
    • Related Report
      2008 Final Research Report
  • [Presentation] 伝達性海綿状脳症におけるシナプス関連蛋白発現の経時的解析2007

    • Author(s)
      佐々木健介、皆木晴彦、岩城徹
    • Organizer
      第48回日本神経病理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Time course of the development of PrP aggregates in a mouse model of prion disease2007

    • Author(s)
      Sasaki K., et. al.
    • Organizer
      Prion 2007
    • Place of Presentation
      Edinburgh、英国
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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