Regulation of higher brain functions by NMDA receptor phosphorylation and Rho GTPases

• Project/Area Number: 19500318
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: NAKAZAWA Takanobu The University of Tokyo, 医科学研究所, 助教 (00447335)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脳・神経 / シグナル伝達 / NMDA 受容体 / Rho ファミリータンパク質 / p250GAPTCGAP / Fyn / チロシンリン酸化 / NMDA受容体 / Rho / p250GAP / TCGAP

Research Abstract

神経系に高発現するRhoGAP蛋白質p250GAPの神経細胞の形態制御における機能について解析を行った。その結果、(1)p250GAPは神経細胞のスパイン形態制御分子であること(2)NMDA型グルタミン酸受容体を介したRhoA の活性調節に重要であること、及び(3)p250GAPのホモログであるTCGAPも神経細胞の形態を制御していることを見出した。

Research Products

• [Journal Article] Regulation of dendritic spine morphology by an NMDA receptor-associated RhoGTPase activating protein, p250GAP (2008)
  • Author(s): Nakazawa T,Kuriu T,Tezuka T,Umemori H,Okabe S, & Yamamoto T.
  • Journal Title: J. Neurochem 105 Pages: 1384-1393
  • Peer Reviewed
• [Journal Article] Loss of Fyn tyrosine kinase on the C57BL/6 genetic background causes hydrocephalus with defects in oligodendrocyte development (2008)
  • Author(s): Goto J, Tezuka T, Nakazawa T, Sagara H & Yamamoto T.
  • Journal Title: Mol. Cell. Neurosci 38 Pages: 203-212
  • Peer Reviewed
• [Journal Article] Activation of Fyn tyrosine kinase in the mouse dorsal hippocampus is essential for contextual fear conditioning (2008)
  • Author(s): Isosaka T, Hattori K, Kida S, Kohno T, Nakazawa T, Yamamoto T, Yagi T & Yuasa S.
  • Journal Title: Eur J. Neurosci 28 Pages: 973-981
  • Peer Reviewed
• [Journal Article] Activation of Fyn tyrosine kinase in the mouse dorsal hippocampus is essential for contextual fear conditioning. (2008)
  • Author(s): Isosaka T
  • Journal Title: Eur J. Neurosci. 28 Pages: 973-981
  • Peer Reviewed
• [Journal Article] Regulation of dendritic spine morphology by an NMDA receptor-associated RhoGTPase activating protein, p250GAP. (2008)
  • Author(s): Nakazawa T
  • Journal Title: J. Neurochem. In press(掲載確定)
  • Peer Reviewed
• [Journal Article] Loss of Fyn tyrosine kinase on the C57BL/6 genetic background causes hydrocephalus with defects in oligodendrocyte developmen (2008)
  • Author(s): Goto J
  • Journal Title: Mol. Cell. Neurosci. In press(掲載確定)
  • Peer Reviewed
• [Journal Article] Neurotensin type 2 receptor in astrocytes is involved in fear memory in mice (2007)
  • Author(s): Yamaguchi R, Wada E, Kamichi S, Yamada D, Maeno H, Delawary M, Nakazawa T, Yamamoto T & Wada K.
  • Journal Title: J. Neurochem 102 Pages: 1669-1676
  • Peer Reviewed
• [Journal Article] MicroRNA modulation of circadian-clock period and entrainment (2007)
  • Author(s): Cheng H-YM, Papp JW, Varlamova O, Dziema H, Russell B, Curfman JP, Nakazawa T, Shimizu K, Okamura H, Impey S & Obrietan K.
  • Journal Title: Neuron 54 Pages: 813-829
  • Peer Reviewed
• [Journal Article] Molecular characterization of a novel RhoGAP, RRC-1 of the nematode Caenorhabditis elegans (2007)
  • Author(s): Delawary M, Nakazawa T, Tezuka T, Sawa M, Iino Y, Takenawa T & Yamamoto T.
  • Journal Title: Biochem. Biophys. Res. Commun 357 Pages: 377-382
  • Peer Reviewed
• [Journal Article] MicroRNA modulation of circadian-clock period and entrainment. (2007)
  • Author(s): Cheng H-YM
  • Journal Title: Neuron 54 Pages: 813-829
  • Peer Reviewed
• [Presentation] Characterization of transmembrane kinase BREK/LMTK2 in endocytic membrane trafficking (2008)
  • Author(s): 井上猛、河成鎮、中澤敬信、須藤和夫、山本雅
  • Organizer: 第31回日本分子生物学会年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 中枢神経系における新規プロトカドヘリンを介したシグナル伝達機構の解析 (2008)
  • Author(s): 星名直祐、手塚徹、中澤敬信、横山一剛、山本雅
  • Organizer: 第31回日本分子生物学会年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Involvement of phosphorylation of NR2B subunit of NMDA receptor at Tyr1472 in maintenance of neuropathic pain (2008)
  • Author(s): Ito S, Mabuchi T, Matsumura S, Katano T, Okuda-Ashitaka E, Ohnishi T, Kuniri S, Endo H, Nakazawa T, Yamamoto T
  • Organizer: 12^<th> World Congress of Pain
  • Place of Presentation: Glasgow
• [Presentation] p250GAP, a brain-enriched RhoGAP, in involved in the NMDAR-mediated signaling (2008)
  • Author(s): 中澤敬信、栗生俊彦、岡部繁男、山本雅
  • Organizer: 第31回日本神経科学学会大会(Neuro2008)
  • Place of Presentation: 東京
• [Presentation] Involvement of NR2A tyrosine phosphorylation in depression related behaviors (2008)
  • Author(s): 手塚徹、谷口幸子、中澤敬信、城山優治、真鍋俊也、山本雅
  • Organizer: 第31回日本神経科学学会大会(Neuro2008)
  • Place of Presentation: 東京
• [Presentation] p250GAP, a brain-enriched RhoGAP, in involved in the NMDAR-mediated signaling (2008)
  • Author(s): 中澤敬信
  • Organizer: 日本神経科学会
  • Place of Presentation: 東京
• [Presentation] Involvement of phosphorylation of NR2B subunit of NMDA receptor at Tyr1472 in maintenance of neuropathic pain (2008)
  • Author(s): Ito S
  • Organizer: 12th World Congress of Pain
  • Place of Presentation: Glasgow
• [Presentation] Involvement of NR2A tyrosine phosphorylation in depression related behaviors (2008)
  • Author(s): 手塚徹、中澤敬信
  • Organizer: 日本神経科学会
  • Place of Presentation: 東京
• [Presentation] 中枢神経系における新規プロトカドヘリンを介したシグナル伝達機構の解析 (2008)
  • Author(s): 星名直祐、中澤敬信
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Characterization of transmembrane Kinase BREK/LMTK2 in endocytic membrane trafficking (2008)
  • Author(s): 井上猛、中澤敬信
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] NMDA受容体チロシンリン酸化による脳高次機能制御 (2007)
  • Author(s): 中澤敬信, 山本雅
  • Organizer: 第30回日本分子生物学会年会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] NMDA受容体チロシンリン酸化による脳高次機能制御 (2007)
  • Author(s): 中澤 敬信
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Regulation of dendritic spine morphology by an NMDA receptor-associated RhoGAP, p250GAP (2007)
  • Author(s): Nakazawa T, Kuriu T, Okabe S, Yamamoto T
  • Organizer: 37th ANNUAL MEETING Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2007-11-06
• [Presentation] Regulation of amygdala functions by NMDAR tyrosine phosphorylation (2007)
  • Author(s): 中澤敬信, デラワリミナ, 山本雅
  • Organizer: 第30回日本神経科学学会大会(Neuro2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Book] グルタミン酸受容体のチロシンリン酸化を介する記憶形成の制御(第45巻11号) (2007)
  • Author(s): 中澤敬信
  • Publisher: 化学と生物
• [Book] NMDA受容体のリン酸化による記憶・学習制御(第25巻1号) (2007)
  • Author(s): 中澤敬信、山本雅
  • Publisher: 実験医学
• [Book] 化学と生物 (2007)
  • Author(s): 中澤 敬信
  • Publisher: 学会出版センター
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/gannsig/member/nakazawa/nakazawa.html
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/gannsig/member/nakazawa/nakazawa.html
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/gannsig/member/nakazawa/nakazawa.html