K+ channel changing cell adhesion-activity by signal transduction and mechanism of synapse formation

• Project/Area Number: 19500321
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Mie University
• Principal Investigator: KAZUSHI Kimura Mie University, 大学院・医学系研究科, 講師 (20314180)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 分子 / 細胞 / 神経生物学 / シナプス / チャネル / 細胞接着 / イオンチャネル

Research Abstract

複雑かつ正確に秩序だった脳の神経回路の形成において、"シナプス形成"はその基本となる重要なステップである。シナプス形成の最初のステップである標的細胞の認識は重要であると考えられる。申請者は、数年来、細胞間接着活性を指標として、新たなシナプス形成に関わる細胞接着分子の探索を試みてきた。その結果、電位依存性カリウムイオンチャネルの一種、KCNH3をその候補として見いだしている。KCNH3は物理的信号(細胞膜電位変化)と化学的信号(cAMP濃度変化)の両方を感知して、シナプス強度を制御する全く新しい機能分子であると考えられる。

Research Products

• [Journal Article] PASK (proline-alanine-rich Ste20-related kinase) binds to tubulin and microtubules and is involved in microtubule stabilization (2008)
  • Author(s): Tsutsumi T, Kosaka T, Ushiro H, Kimura K, Honda T, Kayahara T, Mizoguchi A.
  • Journal Title: Arch Biochem Biophys 477(2) Pages: 267-78
  • Peer Reviewed
• [Journal Article] PASK (proline-alanine-rich Ste20-related kinase) binds to tubulin and microtubules and is involved in microtubule stabilization. (2008)
  • Author(s): Tsutsumi T, Kosaka T, Ushiro H, Kimura K, Honda T, Kayahara T, Mizoguchi A.
  • Journal Title: Arch Biochem Biophys. 477 Pages: 267-278
  • Peer Reviewed
• [Journal Article] TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation (2007)
  • Author(s): Toiyama Y, Mizoguchi A, Kimura K, Hiro J, Inoue Y, Tutumi T, Miki C, Kusunoki M.
  • Journal Title: World J Gastroenterol 13(19) Pages: 2717-21
  • Peer Reviewed
• [Journal Article] Impaired cerebellar development and function in mice lacking CAPS2 a protein involved in neurotrophin release (2007)
  • Author(s): Sadakata T, Kakegawa W, Mizoguchi A, Washida M, Katoh-Semba R, Shutoh F, Okamoto T, Nakashima H, Kimura K, Tanaka M, Sekine Y, Itohara S, Yuzaki M, Nagao S, Furuichi T.
  • Journal Title: J. Neurosci 27(10) Pages: 2472-82
  • Peer Reviewed
• [Journal Article] TTYH2,a human homologue of the Drosophila melanogaster gene tweety,is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation (2007)
  • Author(s): Toiyama, Y, et. al.
  • Journal Title: World J.Gastroenterol 13 Pages: 2717-2721
  • Peer Reviewed
• [Journal Article] Impaired cerebellar development and function in mice lacking CAPS2,a protein involved in neurotrophin release. (2007)
  • Author(s): Sadakata, T, et. al.
  • Journal Title: J.Neurosci. 27 Pages: 2472-2482
  • Peer Reviewed
• [Journal Article] Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer. (2007)
  • Author(s): Okada, Y, et. al.
  • Journal Title: Nat.Biotechnol. 25 Pages: 233-237
  • Peer Reviewed
• [Presentation] The N-linked glycosylation controls the cell surface expression of KCNH3 potassium channel (2008)
  • Author(s): Noma K, Kimura K, Mizoguchi A, Fujiyoshi Y.
  • Organizer: Neuroscience2008
  • Place of Presentation: Washington D.C.
  • Year and Date: 2008-11-19
• [Presentation] 電位依存性KチャネルKCNH3の機能的発現に対する糖鎖修飾の影響 (2008)
  • Author(s): 野間健太郎, 木村一志, 溝口明, 藤吉好則
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29