Role of Syntaxin5 isoforms in neuronal cells under ER stress

• Project/Area Number: 19500327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kyorin University
• Principal Investigator: SUGA Kei Kyorin University, 医学部, 助教 (30306675)
• Co-Investigator(Kenkyū-buntansha): SAITO Ayako 杏林大学, 医学部・その他 (80424109) ; MISHIMA Tatsuya 杏林大学, 医学部, 助教 (40317095)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 神経科学 / 脳・神経 / ストレス / 脳神経疾患 / syntaxin / ER-stress / Golgi / vesicle transport

Research Abstract

The underlying mechanism of how neuronal cells respond to ER stress during the progression of neurodegenerative diseases is poorly understood. We have shown that Syntaxin5 (Syx5) one of the ER-Golgi SNAREs is upregulated under ER stress. The transient upregulation of Syx5 isoforms was observed at the early stages of ER stress response before the cell death pathway have been activated. A subset of ER-Golgi SNAREs was also upregulated by ER stress. These ER-Golgi SNAREs may cooperatively function in the vesicular transport system in neuronal cells.

Research Products

• [Journal Article] Up-regulation of Syntaxin5 protein expression under ER stress (2010)
  • Author(s): K.Suga, A.Saito, T.Mishima, K.Akagawa
  • Journal Title: Bull.Jpn.Soc.Neurochem. Volume: 49(No.2,3) Pages: 631-631
  • Peer Reviewed
• [Journal Article] The Syntaxin5 isoforms Syx5 and Syx5L have distinct effects on the processing of βAPP (2009)
  • Author(s): K.Suga, A.Saito, T.Tomiyama, H.Mori, K.Akagawa
  • Journal Title: J.Biochem. Vol.146, No.6 Pages: 905-915
  • Peer Reviewed
• [Journal Article] Ca^<2+> buffering capacity of mitochondria after oxygen-glucose deprivation in hippocampal neurons (2009)
  • Author(s): K.Tanaka, T.Iijima, T.Mishima, K.Suga, K.Akagawa, Y.Iwao
  • Journal Title: Neurochem.Res. Vol.34 Pages: 221-226
  • Peer Reviewed
• [Journal Article] The Syntaxin5 isoforms Syx5 and Syx5L have distinct effects on the processing of・APP (2009)
  • Author(s): K. Suga, A. Saito, T. Tomiyama, H. Mori, K. Akagawa
  • Journal Title: J.Biochem Vol.146, No.6 Pages: 905-915
  • Peer Reviewed
• [Journal Article] Y. Iwao Ca2+ buffering capacity of mitochondria after oxygen-glucose deprivation in hippocampal neurons. (2009)
  • Author(s): K. Tanaka, T. Iijima, T. Mishima, K. Suga, K. Akagawa
  • Journal Title: Neurochem. Res Vol. 34 Pages: 221-226
  • Peer Reviewed
• [Journal Article] Role of Syntaxin5 isoforms on the processing and trafficking of βAPP in the ER through the Golgi compartments (2009)
  • Author(s): K.Suga, A.Saito, T.Tomiyama, H.Mori, K.Akagawa
  • Journal Title: Bull.Jpn.Soc.Neurochem. 48(No.2,3) Pages: 193-193
  • Peer Reviewed
• [Journal Article] The Syntaxin5 isoforms Syx5 and Syx5L have distinct effects on the processing of βAPP (2009)
  • Author(s): K.Suga, A.Saito, T.Tomiyama, H.Mori, K.Akagawa
  • Journal Title: J.Biochem. 146, No.6 Pages: 905-915
  • Peer Reviewed
• [Journal Article] Ca^<2+> buffering capacity of mitochondria after oxygen-glucose deprivation in hippocampal neurons (2009)
  • Author(s): K. Tanaka, T. Iijima, T. Mishima, K. Suga, K. Akagawa, Y. Iwao
  • Journal Title: Neurochem. Res. 34 Pages: 221-6
  • Peer Reviewed
• [Journal Article] Syntaxin8 has two functionally distinct di-leucine based motif (2008)
  • Author(s): K.Kasai, K.Suga, T.Izumi, K.Akagawa
  • Journal Title: Cell.Mol.Biol.lett. Vol.13 Pages: 144-154
  • Peer Reviewed
• [Journal Article] Calcium loading capacity and morphological changes in mitochondria in an ischemic preconditioned model (2008)
  • Author(s): T.Iijima, K.Tanaka, S.Matsubara, S.Kawakami, T.Mishima, K.Suga, K.Akagawa, Y.Iwao
  • Journal Title: Neurosci.Lett. Vol.448 Pages: 268-272
  • Peer Reviewed
• [Journal Article] Syntaxin8 has two functionally distinct di-leucine based motif. (2008)
  • Author(s): K. Kasai, K. Suga, T. Izumi, K. Akagawa
  • Journal Title: Cell. Mol. Biol. lett Vol.13 Pages: 144-154
  • Peer Reviewed
• [Journal Article] Y. Iwao Calcium loading capacity and morphological changes in mitochondria in an ischemic preconditioned model. (2008)
  • Author(s): T. Iijima, K. Tanaka, S. Matsubara, S. Kawakami, T. Mishima, K. Suga, K. Akagawa
  • Journal Title: Neurosci. Lett Vol.448 Pages: 268-272
  • Peer Reviewed
• [Journal Article] Calcium loading capacity and morphological changes in mitochondria in an ischemic preconditioned model. (2008)
  • Author(s): T. Iijima, K. Tanaka, S. Matsubara, S. Kawakami, T. Mishima, K. Suga, K. Akagawa, Y. Iwao
  • Journal Title: Neurosci Lett. 448 Pages: 268-72
  • Peer Reviewed
• [Journal Article] Distinct effect of Syntaxin5 isoforms on the processing of βAPP (2008)
  • Author(s): K. Suga, A. Saito, T. Tomiyama, H. Mori, K. Akagawa
  • Journal Title: Bull. Jpn. Soc. Neurochem. 47 Pages: 253-253
  • Peer Reviewed
• [Journal Article] Syntaxin8 has two functionally disthinct di-leucine based motif (2008)
  • Author(s): Kazuo Kasai, Kei Suga, Tetsuro Izumi, Kimio Akagawa
  • Journal Title: Cell. Mol. Biol. lett 13 Pages: 144-154
  • Peer Reviewed
• [Journal Article] Effects of 5-HT signaling on cultured hippocampal neurons of HPC-1/syntaxin1A knock-out mouse (2008)
  • Author(s): Takefumi Kofuji, Tomonori Fujiwara, Tatsuya Mishima, Kimio Akagawa
  • Journal Title: J. Physiol. Sci 58 Pages: 128-128
  • NAID: 130005449606
  • Peer Reviewed
• [Journal Article] Effects of Syntaxin5 isoforms on the accumulation of intracellular βAPP and the secretion of Aβ peptides (2007)
  • Author(s): Kei Suga, Ayako Saito, Takami Tomiyama, Hiroshi Mori and Kimio Akagawa
  • Journal Title: Bull. Jpn. Soc. Neurochem 46 Pages: 484-484
  • Peer Reviewed
• [Journal Article] cAMP signaling cascade is reduced in hippocampal LTP of HPC-1/syntaxin 1A knock-out mice (2007)
  • Author(s): Tatsuya Mishima, Tomonori Fujiwara, Takefumi Kofuji, Kimio Akagawa
  • Journal Title: Bull. Jpn. Soc. Neurochem 46 Pages: 575-575
  • Peer Reviewed
• [Journal Article] Deletion of HPC-1/syntaxin lA gene causes autistic behavior in mice (2007)
  • Author(s): Tomonori Fujiwara, Takefumi Kofuji, Tatsuya Mishima, Takeo Yoshikawa, Kimio Akagawa
  • Journal Title: Bull. Jpn. Soc. Neurochem 46 Pages: 498-498
  • Peer Reviewed
• [Presentation] Up-regulation of Syntaxin5 protein expression under ER stress (2010)
  • Author(s): 須賀圭,齋藤綾子、三嶋竜弥、赤川公朗
  • Organizer: 第53回日本神経化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Syntaxin5の発現量は小胞体ストレスにより上昇する (2010)
  • Author(s): 須賀圭, 齋藤綾子, 三嶋竜弥, 赤川公朗
  • Organizer: 第53回日本神経化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] 小胞体ストレスにおけるSNARE蛋白質の役割 (2009)
  • Author(s): 須賀圭, 齋藤綾子, 三嶋竜弥, 赤川公朗
  • Organizer: 第38回杏林医学会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-21
• [Presentation] 小胞体とゴルジ体での・APPのプロセシングと細胞内輸送におけるSyntaxin5アイソフォームの役割 (2009)
  • Author(s): 須賀圭,齋藤綾子,富山貴美,森啓,赤川公朗
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-22
• [Presentation] 小胞体とゴルジ体での・APPのプロセシングと細胞内輸送におけるSyntaxin5アイソフォムの役割 (2009)
  • Author(s): 須賀圭, 齋藤綾子, 富山貴美, 森啓, 赤川公朗
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-22
• [Presentation] 小胞体とゴルジ体でのβAPPのプロセシングと細胞内輸送におけるSyntaxin5アイソフォームの役割 (2009)
  • Author(s): 須賀圭, 齋藤綾子, 富山貴美, 森啓, 赤川公朗
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-22
• [Presentation] Distinct effect of Syntaxin5 isofonus on the processing of βAPP (2008)
  • Author(s): 須賀圭, 齋藤綾子, 富山貴美, 森啓, 赤川公朗
  • Organizer: 第51回日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-13
• [Presentation] Effects of 5-HT signaling on cultured hippocampal neurons of HPC-1/syntaxin1A knock-out mouse (2008)
  • Author(s): 小藤 剛史, 藤原 智徳, 三嶋 竜弥, 赤川 公朗
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] アルツハイマー病関連蛋白質のプロセッシングと輸送におけるSyntaxin5の役割 (2007)
  • Author(s): 須賀 圭, 赤川 公朗
  • Organizer: 第36回杏林医学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-17
• [Presentation] cAMP signaling cascade is reduced in hippocampal LTP of HPC-1/synta xin1A knock-out mice (2007)
  • Author(s): 三嶋 竜弥, 藤原 智徳, 小藤 剛史, 赤川 公朗
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-12
• [Presentation] Effects of Syntaxin5 isoforms on the accumulation of intracellular βAPP and the secretion of Aβ peptides (2007)
  • Author(s): 須賀 圭, 齋藤 綾子, 富山 貴美, 森 啓, 赤川 公朗
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] Deletion of HPC-1/syntaxin1A gene causes autistic behavior in mice (2007)
  • Author(s): 藤原 智徳, 小藤 剛史, 三鳴 竜弥, 吉川 武男, 赤川 公朗
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
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