Three-dimensional structural analysis of Creutzfeldt-Jakob disease-related molecules

• Project/Area Number: 19500339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: MORONE Nobuhiro National Center of Neurology and Psychiatry, 神経研究所・微細構造研究部, 室長 (50399680)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脳神経疾患 / タンパク質 / 細胞・組織 / 蛋白質

Research Abstract

クロイツフェルト・ヤコブ病の感染に関わる分子としては、プルシナー博士による「タンパク質仮説」が有力視されているが、詳しい素過程は解明されていない。本研究では、この診断マーカーである「14-3-3ζタンパク質」がミトコンドリアに濃縮していることや、シャペロンタンパク質との複合体形成が「膜中コレステロール濃度の低下」や「アクチンフィラメントの脱重合」により解離することを明らかにした。

Research Products

• [Journal Article] Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography. (2008)
  • Author(s): Morone N, Nakada C, Umemura Y, Usukura J, Kusumi A.
  • Journal Title: Methods Cell Biol. 88 Pages: 207-36
• [Journal Article] athematical morphology に基づくバイオイメージからの構造情報の抽出解析 (2008)
  • Author(s): 木森義隆, 諸根信弘, 片山栄作
  • Journal Title: 顕微鏡 44 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research. (2008)
  • Author(s): Kobayashi T, Morone N, Kashiyama T, Oyamada H, Kurebayashi N, Murayama T.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography (2008)
  • Author(s): Morone N
  • Journal Title: Methods Cell Biol. 88 Pages: 207-236
  • Peer Reviewed
• [Journal Article] Mathematical morphology に基づくバイオイメージからの構造情報の抽出と解析 (2008)
  • Author(s): 木森義隆
  • Journal Title: 顕微鏡 44 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research (2008)
  • Author(s): Kobayashi T
  • Journal Title: PLoS ONE 3, e3822 Pages: 19048102-19048102
  • Peer Reviewed
• [Journal Article] SCRAPPER-dependent ubiquitination of active zone protein RIMI regulates synaptic vesicle release (2007)
  • Author(s): Yao I
  • Journal Title: Cell 130 Pages: 943-57
  • Peer Reviewed
• [Journal Article] 電子線<フリーズレプリカ>トモグラフィー法による細胞膜骨格の3次元イメージング (2007)
  • Author(s): 諸根 信弘
  • Journal Title: 顕微鏡 42 Pages: 150-154
  • NAID: 10020010056
  • Peer Reviewed
• [Presentation] GPI-anchored Protein's complex and membrane skeleton in human neural progenitors as revealed by electron microscopy (2008)
  • Author(s): Morone N
  • Organizer: The 48th annual meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco USA
  • Year and Date: 2008-12-14
• [Presentation] GPI-Anchored Protein And Membrane Structure Inside Human Neural Progenitors (2008)
  • Author(s): Morone N
  • Organizer: The 5^<th> international Forum on Oxidative Stress and Aging
  • Place of Presentation: Ancona Italy
  • Year and Date: 2008-09-13
• [Presentation] The structural interplay between the plasma membrane and cytoskeletons in neural svstems: a deep-etch EM research (2008)
  • Author(s): N.Morone
  • Organizer: The 38th Seiriken/Sokendai International Conference, "Stock and flow of functional molecules in svnapse"
  • Place of Presentation: Okazaki Japan
  • Year and Date: 2008-03-19
• [Presentation] 電子線<フリーズレプリカ>トモグラフィー法で細胞膜構造を3次元解析する (2008)
  • Author(s): 諸根 信弘
  • Organizer: 日本顕微鏡学会関東支部講演会
  • Place of Presentation: 目黒 東京
  • Year and Date: 2008-03-08
• [Presentation] Electron freeze-replica tomography correlated with a single-moleculeimaging: a structural analysis for the plasma membrane (2008)
  • Author(s): N.Morone
  • Organizer: The First iCeMS International Symposium The Eleventh MEMBRANE RESEARCH FORUM
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2008-02-20
• [Presentation] GPI-Anchored Protein And Membrane Structure Inside Human Neural Progenitors. (2008)
  • Author(s): Morone N, Wakui F, Kohno T, Yamamura T, Satoh J, and Yuasa S.
  • Organizer: The 5^<th> international Forum on Oxidative Stress and Aging
  • Place of Presentation: Ancona Italy.
• [Presentation] GPI-anchored Protein's complex and membrane skeleton in human neural progenitors as revealed by electron microscopy. (2008)
  • Author(s): Morone N, Wakui F, Kohno T, Kimori Y, Yuasa S.
  • Organizer: The 48th annual meeting of the American Society for Cell Biology
  • Place of Presentation: Francisco USA.
• [Presentation] 細胞膜インターフェイスの構造組織化 (2007)
  • Author(s): 諸根 信弘
  • Organizer: 日本顕微鏡学会生体構造解析分科会2007年度研究討論会VII
  • Place of Presentation: 晴海 東京
  • Year and Date: 2007-12-20
• [Presentation] GPI-anchored protein localizes with 14-3-3 and HSP60 on the mitochondria in human neural progenitors (2007)
  • Author(s): N.Morone, J.Satoh, F.Wakui, T.Yamamura, S.Yuasa
  • Organizer: American Society for Cell Biology 47th Amual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-12-05
• [Presentation] Three-dimensional reconstruction of the membrane skeleton lust beneath the plasma membrane by electron tomography (2007)
  • Author(s): N.Morone, T.K.Fujiwara, K.Murase, R.S.Kasai, H.Ike, S.Yuasa, J.Usukura, and A.Kusumi
  • Organizer: Biochemistry and Molecular Biology 2007
  • Place of Presentation: Yokohama Japan
  • Year and Date: 2007-10-11
• [Presentation] Electron tomography reveals the plasma membrane compartmentalizatiorn as its three dimensional interplay between membrane and cytoskeletal systems (2007)
  • Author(s): N.Morone
  • Organizer: The American Sodety for Cell Biology & Euro pean Cytoskeleton Forum
  • Place of Presentation: Dijon France
  • Year and Date: 2007-06-28
• [Book] Anomalous Transport : Foundations and Applications (edited by Rainer Klages, Gunter Radons, Igor M. Sokolov) (2008)
  • Author(s): Kusumi A
  • Total Pages: 30
  • Publisher: Paradigm Shift of the Molecular Dynamics Concept in the Cell Membrane : High-Speed Single-Molecule Tracking Revealed the Partitioning of the Cell Membrane