Analysis and generation of functional molecules to modulate neutral amino acid transporters
| Project/Area Number |
19500341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SHIGERI Yasushi National Institute of Advanced Industrial Science and Technology, 健康工学研究センター, 主幹研究員 (90357187)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Ryuichi 北海道大学, 水産学部, 教授 (20265721)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | トランスポーター / 阻害剤 / 神経伝達物質と受容体 / アミノ酸 / 基質 |
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| Research Abstract |
Alanine-serine-cysteine transporters (ASCT1, 2) are neutral amino acids transporters. Their representative substrates are alanine, serine and cysteine. Since th ere is no specific inhibitors or substrates for ASCT1 and ASCT2, their physiological functions are quite mystery. To develop their specific compounds to modulate their functions, high-throughput screening is essential. Therefore, we made two approaches as follows. First, we looked for host cells with lower level of endogenous ASCT1 or ASCT2. We checked CHO cells, HEK293 cells, 3T3 cells and other cells. But all of the cells that we examined showed higher level of ASCTs expression. Second, we tried the cloning of human and rat type ASCTs. Cloned ASCTs were integrated into the expression vectors for their expressions in mammalian cells and Xenopus oocytes.
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Report
(4 results)
Research Products
(16 results)
