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Optical analysis of functional development of the auditory andvestibular neural circuits

Research Project

Project/Area Number 19500346
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SATO Katsushige  Tokyo Medical and Dental University, 大学院医歯学総合研究科, 講師 (80291342)

Co-Investigator(Kenkyū-buntansha) 佐藤 容子  関東学院大学, 人間環境学部, 教授 (70251501)
Co-Investigator(Renkei-kenkyūsha) SATO Yoko  関東学院大学, 人間環境学部, 教授 (70251501)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords光学計測 / 膜電位感受性色素 / 中枢神経系 / 機能発生 / embryo / 聴覚 / 平衡覚 / 生体染色 / 鶏胚
Research Abstract

本研究は、発生初期胚脳幹の聴覚・平衡反射神経回路網に焦点をあて、神経電位活動の光学的イメージング法を用いてその機能構築過程を解析したものである。孵卵5.5-8日の鶏胚から第VIII脳神経を付けたままの標本を作製し、膜電位感受性色素で染色して、聴神経刺激に対する光学応答を記録した。解剖学的研究との比較から、脳幹内での応答領域と神経核の対応を検討した。また、各神経核におけるシナプス伝達の発現時期は、これまで考えられていた発生段階よりもかなり早いことが明らかとなった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (55 results)

All 2009 2008 2007 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (28 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Origin of the earliest correlatedneuronal activity in the chick embryo revealedby optical imaging with voltage-sensitive dyes2009

    • Author(s)
      Momose-Sato, Y., Mochida, H., Kinoshita, M
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 1-13

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediatehindbrain correlated activity in the chick embryo2009

    • Author(s)
      Mochida, H., Sato, K., Momose-Sato, Y
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 14-30

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo.2009

    • Author(s)
      Mochida, H., et al.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 14-30

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Opticalanalysis of neural circuit formation in theembryonic brain2008

    • Author(s)
      Sato. K., Momose-Sato, Y
    • Journal Title

      Clinical and ExperimentalPharmacology and Physiologya 35

      Pages: 706-713

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording toimage the functional development of neuronalcircuits in vertebrate embryos2008

    • Author(s)
      Glover, J. C., Sato, K., Momose-Sato, Y
    • Journal Title

      Developmental Neurobiology 68

      Pages: 804-816

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Processing of sting pheromone and itscomponents in the antennal lobe of the workerhoneybee2008

    • Author(s)
      Wang, S., Sato, K., Giurfa, M., Zhang, S
    • Journal Title

      Journal of Insect physiology 54

      Pages: 833-841

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical analysis of neural circuit formation in the embryonic brain.2008

    • Author(s)
      Sato. K., Momose-Sato, Y.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 35

      Pages: 706-713

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos.2008

    • Author(s)
      Glover, J. C., et al.
    • Journal Title

      Developmental Neurobiology 68

      Pages: 804-816

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Processing of sting pheromone and its components in the antennal lobe of the worker honeybee2008

    • Author(s)
      Wang, s., et al.
    • Journal Title

      Journal of Insect Physiology 54

      Pages: 833-841

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization waves ofmultiple origins in the embryonic rat CNS2007

    • Author(s)
      Momose-Sato, Y., Sato, K., Kinoshita, M
    • Journal Title

      European Journal of Neuroscience 25

      Pages: 929-944

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical mapping of spatiotemporalemergence of functional synaptic connections in the embryonic chick olfactory pathway2007

    • Author(s)
      Sato, K., Kinoshita, M., Momose-Sato, Y
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of vagal afferent projectionscircumflex to the obex in the embryonic chickbrainstem visualized with voltage-sensitive dyerecording2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K
    • Journal Title

      Neuroscience 148

      Pages: 140-150

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] GABA-induced intracellular Ca^<2+>elevation in the embryonic chick brainstem slice2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in thechick brainstem as revealed by voltage-sensitivedye recording2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K
    • Journal Title

      Neuroscience Letters 423

      Pages: 138-142

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A comparative studyof bioradiography in human brain slices andpreoperative PET imaging2007

    • Author(s)
      Sasaki, T., Nariai, T., Maehara, T., Sato, K., Oda, K., Ishii, K
    • Journal Title

      Brain Research 1142

      Pages: 19-27

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      European Journal of Neuroscience 25

      Pages: 929-944

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience 148

      Pages: 140-150

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical mapping of spatiotempora1 emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., et. al.
    • Journal Title

      Neuroscience 144

      Pages: 334-1346

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in the chick brainstem as revealed by voltage-sensitive dye recording.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience Letters 423

      Pages: 138-142

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GABA-induced intracellular Ca2+ elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., et. al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki, T., et. al.
    • Journal Title

      Brain Research 1142

      Pages: 19-27

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos.

    • Author(s)
      Glover, et. al.
    • Journal Title

      Developmental Neurobiology (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical analysis of neural circuit formation in the embryonic brain.

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regional gradient of neuronal excitability togenerate spontaneous activity in the hindbrain of the chick embryo2008

    • Author(s)
      Kinoshita, M., Momose-Sato, Y., Sato, K
    • Organizer
      6th FENS Forum of EuropeanNeuroscience
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2008 Final Research Report
  • [Presentation] Functionalorganization of the glossopharyngeal and vagusnerve-related nuclei in the embryonic ratbrainstem : Optical mapping withvoltage-sensitive dyes2008

    • Author(s)
      Sato, K., Momose-Sato, Y
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Switching of the transmitters that mediatehindbrain correlated activity in the chick embryo2008

    • Author(s)
      Momose-Sato, Y., Mochida, H., Sato, K
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Neuronal excitability to generate spontaneousactivity in the medulla and spinal cord of thechick embryo2008

    • Author(s)
      Kinoshita, M., Momose-Sato, Y., Sato, K
    • Organizer
      Society for Neuroscience 38thAnnual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Maehara, T., Inaji, M., Nariai, T., Sato, K. andOhno, K2008

    • Author(s)
      Maehara, T., Inaji, M., Nariai, T., Sato, K. Ohno, K
    • Organizer
      AmericanEpilepsy Society 62nd Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹迷走神経回路網の機能発達過程の光学的解析2008

    • Author(s)
      佐藤勝重, 木下雅恵, 佐藤容子
    • Organizer
      第85回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚中枢神経系における脱分極波のoriginの同定 : 光学的イメージングによる解析2008

    • Author(s)
      佐藤容子, 持田啓, 木下雅恵, 佐藤勝重
    • Organizer
      第85回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 初期胚脳幹における機能的神経回路網形成に対する光学的アプローチ2008

    • Author(s)
      佐藤勝重
    • Organizer
      第85回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹における迷走神経求心線維の機能発達過程の光学的解析2008

    • Author(s)
      佐藤勝重, 木下雅恵, 佐藤容子
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 光学的イメージングによる鶏胚中枢神経系における脱分極波のoriginの同定2008

    • Author(s)
      佐藤容子, 持田啓, 木下雅恵, 佐藤勝重
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚後脳における神経興奮性の部位差2008

    • Author(s)
      木下雅恵, 佐藤容子, 佐藤勝重
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Functional organization of the glossopharyngeal and vagus nerve-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes.2008

    • Author(s)
      Sato, K., Momose-Sato, Y.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo.2008

    • Author(s)
      Momose-Sato, Y., et al.
    • Organizer
      Society for Neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Depolarization waves in the embryonic CNSvisualized with voltage-sensitive dye imaging2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K
    • Organizer
      7th IBRO World Congress of Neuroscience
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of vagal afferent projectionscircumflex to the obex in the embryonic chickbrainstem visualized with voltage-sensitive dyerecording2007

    • Author(s)
      Sato, K., Kinoshita, M., Momose-Sato, Y
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] The origin of the depolarizationwave at an early phase of the chick developmentrevealed by optical imaging with avoltage-sensitive dye2007

    • Author(s)
      Momose-Sato, Y., Mochida, H., Kinoshita, M., Sato, K
    • Organizer
      Society for Neuroscience37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Regulation of spontaneous rhythmic activities inthe caudal medulla by the spinal cord in the chickembryo2007

    • Author(s)
      Kinoshita, M., Momose-Sato, Y., Sato, K
    • Organizer
      Society for Neuroscience 37th AnnualMeeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Intraoperativeintrinsic optical imaging in brain tumorsurgery2007

    • Author(s)
      Inaji, M., Nariai, T., Sato, K., Momose-Sato, Y., Maehara, T., Ohno, K
    • Organizer
      6th Congress of the Asian Society forStereotactic, Functional and Computer Assisted Neurosurgery
    • Place of Presentation
      Fujiyoshida, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Optical signalof human cerebral cortex induced by soundstimulation as monitoring tool of parasylvianstructure of dominant hemisphere2007

    • Author(s)
      Nariai, T., Sato, K., Maehara, T., Inaji, M., Hosoda, C., Ishii, K., Ohno, K
    • Organizer
      The 13thAnnual Meeting of the Organization for HumanBrain Mapping
    • Place of Presentation
      Chicago, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] 光学的イメージング法による鶏胚聴覚神経回路網の機能発達過程の解析2007

    • Author(s)
      佐藤勝重, Glover J. C., 佐藤容子
    • Organizer
      第84回日本生理学会
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット胚中枢神経系における自発性脱分極波のorigin の光学的解析2007

    • Author(s)
      佐藤容子, 佐藤勝重, 木下雅恵
    • Organizer
      第84回日本生理学会
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report
  • [Presentation] 鶏胚脳幹聴覚神経回路網の機能発達過程の光学的解析2007

    • Author(s)
      佐藤勝重、Glover J.C., 佐藤容子
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット胚中枢神経系における自発興奮波の時空間パターンとoriginの同定 : 光学的イメージングによる解析2007

    • Author(s)
      佐藤容子, 佐藤勝重, 木下雅恵
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] ラット皮質線条体における膜電位感受性色素による神経伝達イメージング2007

    • Author(s)
      稲次基希, 佐藤勝重, 成相直, 百瀬-佐藤容子, 大野喜久郎
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Chick終脳における刷り込み成立に伴う神経回路活性の解析2007

    • Author(s)
      中森智啓, 佐藤勝重, 田中光一, 浜崎浩子
    • Organizer
      第30回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording.2007

    • Author(s)
      Sato, et. al.
    • Organizer
      Society for Neuroscienc 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] The origin of the depolarization wave at an early phase of the chick development revealed by optical imaging with a voltage-sensitive dye.2007

    • Author(s)
      Momose-Sato, et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Regulation of spontaneous rhythmic activities in the caudal medulla by the spinal cord in the chick embryo.2007

    • Author(s)
      Kinoshita, et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2007 Annual Research Report
  • [Book] H. Approaches to the visualization of neuralpathways in chick imprinting. In : NeuralPathways : New Research. Ed(in press)

    • Author(s)
      Maekawa, F., Sato, K., Ohki-Hamazaki, H
    • Publisher
      Columbus, F. Nova Science Publishers
    • Related Report
      2008 Final Research Report
  • [Book] Neural Pathways : New Research

    • Author(s)
      Maekawa, F., et al.
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2008 Annual Research Report
  • [Book] Neural Pathways: New Research

    • Author(s)
      Maekawa, F., et. al.
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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