Correlation between olfactory stimulation and transduction cascade within the sensory cilia

• Project/Area Number: 19500350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Osaka University
• Principal Investigator: KURAHASHI Takashi Osaka University, 大学院・生命機能研究科, 教授 (90225251)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Ca / cAMP / 嗅覚 / ケージド化合物 / 繊毛 / 嗅細胞 / ナノチューブ / パッチクランプ / レーザー / 紫外光

Research Abstract

Olfaction starts at the olfactory sensory cilia which have nano-scale structure. In the olfactory transduction cascade, it is an important because Ca can cause olfactory adaptation and non-linear signal amplification. In this project, we solve the problem that had been left conventionally by monitoring the molecular dynamics.

Research Products

• [Journal Article] Mechanism of olfactory masking in the sensory cilia. (2009)
  • Author(s): Takeuchi H, Ishida H, Hikichi S, Kurahashi T.
  • Journal Title: Journal of General Physiology. 133 Pages: 583-60
• [Journal Article] Mechanism of olfactory masking in the sensory cilia (2009)
  • Author(s): Takeuchi H, Ishida H, Hikichi S, Kurahashi T.
  • Journal Title: Journal of General Physiology 133 Pages: 583-601
  • Peer Reviewed
• [Journal Article] Distribution, amplification, and summation of cyclic nucleotidesensitivities within single olfactory sensory cilia. (2008)
  • Author(s): Takeuchi H, Kurahashi T
  • Journal Title: Journal of Neuroscience 28 Pages: 766-775
  • Peer Reviewed
• [Journal Article] 嗅覚ナノテクノロジーの夜明け (2008)
  • Author(s): 倉橋 隆
  • Journal Title: AromaResearch 09 Pages: 34-37
  • NAID: 40016096087
  • Peer Reviewed
• [Journal Article] Distribution, amplification, and summation of cyclic nucleotide sensitivities within single olfactory sensory cilia. (2008)
  • Author(s): Hiroko Takeuchi, Takashi Kurahashi
  • Journal Title: Journal of Neuroscience. 28 Pages: 766-775
  • Peer Reviewed
• [Presentation] 嗅繊毛内におけるセカンドメッセンジャーの散制限:電流解析と可視化. (2009)
  • Author(s): 竹内裕子、倉橋隆
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] 塩酸コカインによる嗅細胞Na+電流の抑制作用と安全性検討. (2009)
  • Author(s): 玉利健悟、竹内裕子、小林正佳、倉橋隆、山本哲朗
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] 嗅覚マスキングと匂い物質による電位依存性Kチャネルの抑制. (2009)
  • Author(s): 吉田太一、竹内裕子、倉橋隆
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] 嗅覚マスキングと匂い物質による電位依存性Kチャネルの抑制 (2009)
  • Author(s): 吉田太一, 竹内裕子, 倉橋隆
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] 塩酸コカインによる嗅細胞Na+電流の抑制作用と安全性検討 (2009)
  • Author(s): 玉利健悟, 竹内裕子, 小林正佳, 倉橋隆, 山本哲朗
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] 嗅繊毛内におけるセカンドメッセンジャーの拡散制限 : 電流解析と可視化 (2009)
  • Author(s): 竹内裕子, 倉橋隆
  • Organizer: 近畿生理学談話会
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-12-13
• [Presentation] Nano-Manipulation of Olfactory Sensation. (2009)
  • Author(s): Hiroko Takeuchi, Takashi Kurahashi, Hirohiko Ishida, Satoshi Hikichi.
  • Organizer: IUPS(国際生理科学連合)大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-08-01
• [Presentation] Visualization and quantitative analysis of the nano-scale olfactory sensory cilia. (2009)
  • Author(s): Yukako Kishino, Hiroko Takeuchi, Takashi Kurahashi.
  • Organizer: IUPS(国際生理科学連合)大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-08-01
• [Presentation] Visualization and quantitative analysis of the nano-scale olfactory sensory cilia (2009)
  • Author(s): Yukako Kishino, 竹内裕子, 倉橋隆
  • Organizer: IUPS (国際生理科学連合) 大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-08-01
• [Presentation] Nano-Manipulation of Olfactory Sensation (2009)
  • Author(s): 竹内裕子, 倉橋隆, Hirohiko Ishida, Satoshi Hikichi
  • Organizer: IUPS (国際生理科学連合) 大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-08-01
• [Presentation] Mechanism of olfactory masking in the sensory cilia. (2009)
  • Author(s): Hiroko Takeuchi, Takashi Kurahashi
  • Organizer: IUPS(国際生理科学連合)大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-30
• [Presentation] Suppression by odorants of newt olfactory voltage-gated ion channels of receptor cells. (2009)
  • Author(s): Taichi Yoshida, Hiroko Takeuchi, Takashi Kurahashi
  • Organizer: IUPS(国際生理科学連合)大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-30
• [Presentation] Suppression by odorants of voltage-gated ion channels of newt olfactory receptor cells (2009)
  • Author(s): 吉田太一, 竹内裕子, 倉橋隆
  • Organizer: IUPS (国際生理科学連合) 大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-30
• [Presentation] Mechanism of olfactory masking in the sensory cilia (2009)
  • Author(s): 竹内裕子, 倉橋隆
  • Organizer: IUPS (国際生理科学連合) 大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-30
• [Presentation] Single K channels in the newt olfactory receptor neuron. (2009)
  • Author(s): Ryo Sakane, Takashi Kurahashi
  • Organizer: IUPS(国際生理科学連合)大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-29
• [Presentation] Single K channels in the newt olfactory receptor neuron (2009)
  • Author(s): Ryo Sakane, 倉橋隆
  • Organizer: IUPS (国際生理科学連合) 大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-29
• [Presentation] Photolysis of Caged Calcium in the Olfactory Sensory Cilia (2009)
  • Author(s): 竹内 裕子, 倉橋隆
  • Organizer: 日本生理学会
  • Place of Presentation: 新宿京王プラザホテル
• [Presentation] MS-Windows application executing the non-stationary noise analysis of whole-cell transient current (2009)
  • Author(s): 菅原亜弓, 大井華子, 竹内 裕子, 倉橋隆
  • Organizer: 日本生理学会
  • Place of Presentation: 新宿京王プラザホテル
• [Presentation] ナノバイオマスキングの分子メカニズム解明 (2008)
  • Author(s): 竹内裕子, 倉橋隆, 引地聰
  • Organizer: JASTS
  • Place of Presentation: 富山市民会館
  • Year and Date: 2008-09-22
• [Presentation] ナノスケール嗅繊毛内Ca制御とCl(Ca)解析 (2008)
  • Author(s): 竹内裕子, 倉橋隆
  • Organizer: 第101回 近畿生理学談話会
  • Place of Presentation: 国立循環器病センター
  • Year and Date: 2008-09-16
• [Presentation] 単離嗅細胞における電位依存性Na十チャネルの定量的解析 (2008)
  • Author(s): 大井華子, 菅原亜弓, 竹内裕子, 倉橋隆
  • Organizer: 第101回 近畿生理学談話会
  • Place of Presentation: 国立循環器病センター
  • Year and Date: 2008-09-16
• [Presentation] A dawn for the nano-olfactory technology : from theories to applications (2008)
  • Author(s): Takashi Kurahashi, Hiroko Takeuchi
  • Organizer: ECRO
  • Place of Presentation: スロベニア・ポルトロズ
  • Year and Date: 2008-09-04
• [Presentation] Visualization, manipulation and recording of nanotube olfactory cilia (2008)
  • Author(s): Hiroko Takeuchi, Takashi Kurahashi
  • Organizer: ISOT
  • Place of Presentation: アメリカ・サンフランシスコ
  • Year and Date: 2008-07-26
• [Presentation] Mechanism of Olfactory Masking (2008)
  • Author(s): Takashi Kurahashi. Hiroko Takeuchi, Hirohiko Ishida& Satoshi Hikichi
  • Organizer: ISOT
  • Place of Presentation: アメリカ・サンフランシスコ
  • Year and Date: 2008-07-26
• [Presentation] Correlation betwe4en human masking and odorant suppression of cell responses and voltage-dependent currents (2008)
  • Author(s): Kentaro Kumihashi, Hirohiko Ishida, Hanako Oi, Takashi Kurahashi. & Atsushi Ohuchi
  • Organizer: ISOT
  • Place of Presentation: アメリカ・サンフランシスコ
  • Year and Date: 2008-07-26
• [Presentation] 嗅細胞シリアにおけるケージドカルシウムの光乖離 (2008)
  • Author(s): 竹内裕子・倉橋隆
  • Organizer: 日本生理学会
  • Place of Presentation: 生理学会大会(東京)
  • Year and Date: 2008-03-26
• [Presentation] A new testing for sensory evaluation of olfactory masking agents (2008)
  • Author(s): Hirohiko Ishida, Atsushi Katayama, Takashi Kurahashi, & Satoshi Hikichi
  • Organizer: ECRO
  • Place of Presentation: スロベニア・ポルトロズ
• [Presentation] CNG block may reduce the olfactory sensitivity (2008)
  • Author(s): Takashi Kurahashi. Hiroko Takeuchi. Hirohiko Ishida, & Satoshi Hikichi
  • Organizer: ECRO
  • Place of Presentation: スロベニア・ポルトロズ
• [Presentation] Visualization, manipulation and recording of nanotube olfactory cilia (2008)
  • Author(s): Hiroko Takeuchi, Takashi Kurahashi
  • Organizer: ECRO
  • Place of Presentation: スロベニア・ポルトロズ
• [Presentation] Spatial Distribution of Transduction System with Nano-scale Resolution in Living Olfactory Cilia (2007)
  • Author(s): 竹内裕子・倉橋隆
  • Organizer: AchemS (The Association for Chemoreception Sciences)
  • Place of Presentation: AchemS meeting(アメリカ・フロリダ)
  • Year and Date: 2007-04-30
• [Book] The Senses (2008)
  • Author(s): Hiroko Takeuchi & Takashi Kurahashi
  • Total Pages: 11
  • Publisher: Elsevier
• [Book] The Senses: A Comprehensive Reference, Six-Volume Set, 1-6 (Volume 4: Olfaction & Taste 4.28: Signal Transduction in the Olfactory Receptor Cell) (2008)
  • Author(s): Editoted by Allan Basbaum, et. al. (H Takeuchi, T Kurahashi)
  • Total Pages: 4640
  • Publisher: Elsevier
• [Remarks]
  • URL: http://saturn.bpe.es.osaka-u.ac.jp/kura_pub/kurahasi_x.html
• [Patent(Industrial Property Rights)] 電位依存性カチオンチャネル阻害剤 (2008)
  • Inventor(s): 倉橋 隆
  • Industrial Property Rights Holder: 大阪大学, 花王株式会社
  • Filing Date: 2008-04-11
• [Patent(Industrial Property Rights)] 電位依存性カチオンチャネル阻害剤 (2008)
  • Inventor(s): 倉橋 隆
  • Industrial Property Rights Holder: 大阪大学, 花王株式会社
  • Filing Date: 2008-12-01
• [Patent(Industrial Property Rights)] CNGチャネル阻害による嗅覚感度低下剤 (2008)
  • Inventor(s): 倉橋 隆
  • Industrial Property Rights Holder: 大阪大学, 花王株式会社
  • Filing Date: 2008-08-29
• [Patent(Industrial Property Rights)] 嗅覚感度抑制剤のスクリーニング法 (2008)
  • Inventor(s): 倉橋 隆
  • Industrial Property Rights Holder: 花王株式会社
  • Filing Date: 2008-04-11