Quantitative Evaluation of myofilaments remodeling during relaxation-contraction cycling in smooth muscle cells.
| Project/Area Number |
19500356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
WATANABE Masaru Tokyo Medical University, 大学院・人間健康科学研究科, 教授 (60191798)
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| Co-Investigator(Kenkyū-buntansha) |
石田 行知 文京学院大学, 保健医療学部, 教授 (50092135)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Masako 東京慈恵会医科大学, 医学部, 助教 (30328314)
ISHIDA Yukisato 文京学院大学, 保健医療技術学部, 教授 (50092135)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 平滑筋 / 筋収縮タンパク質 / 筋フィラメント / リモデリング / X線回折 / ATP / 収縮タンパク質 / 赤道反射 / スキンド標本 / 太いフィラメント / 細いフィラメント / 収縮 / 弛緩 / 硬直 / ミオシン / アクチン |
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| Research Abstract |
To quantitatively evaluate the relation between myofilament remodeling and mechanical properties in vertebrate smooth muscle cells, by using electron microscopy, florescent resonance energy transfer (FRET) analysis and conventional electrophoresis techniques, we measured both the contractile force and changes in the myofilament structure of the same preparations of skinned (cell membrane permeabilized) taenia cecum. Also an X-ray diffraction study using skinned taenia cecum showed dynamic changes in the lattice structure originated from myofilament arrangement during contraction-relaxation cycles.
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Report
(6 results)
Research Products
(31 results)
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[Journal Article] Blebbistatin, a myosin II inhibitor, suppresses contraction and disrupts contractile filaments organization of skinned taenia cecum from guinea pig2010
Author(s)
Watanabe M, Yumoto M, Tanaka H, Wang HH, Katayama T, Yoshiyama S, Black J, Thatcher SE, Kohama K.
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Journal Title
Am J Physiol Cell Physiol 298
Pages: 1118-1126
Related Report
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[Journal Article] Blebbistatin, a myosin II inhibitor, suppresses contraction and disrupts contractile filaments organization of skinned taeniacecum from guinea pig2010
Author(s)
Watanabe M, Yumoto M, Tanaka H, Wang HH, Katayama T, Yoshiyama S, Black J, Thatcher SE, Kohama K.
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Journal Title
Am J Physiol Cell Physiol
Volume: 298
Pages: 1118-1126
Related Report
Peer Reviewed
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[Journal Article] Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain.
Author(s)
Katayama T, Watanabe M, Tanaka H, Hino M, Miyakawa T, Ohki T, Ye LH, Xie C, Yoshiyama S, Nakamura A, Ishikawa R, Tanokura M, Oiwa K, Kohama K.
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Journal Title
Am J Physiol Am J Physiol Heart Circ Physiol. 298
Related Report
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[Journal Article] Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain
Author(s)
Katayama T, Watanabe M, Tanaka H, Hino M, Miyakawa T, Ohki T, Ye LH, Xie C, Yoshiyama S, Nakamura A, Ishikawa R, Tanokura M, Oiwa K, Kohama K
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Journal Title
Am J Physiol Am J Physiol Heart Circ Physiol 298
Related Report
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