Analysis of brain functions of PACAP using its specific receptor PAC1 transgenic mice.
| Project/Area Number |
19500368
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Showa University |
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Principal Investigator |
ARATA Satoru Showa University, 遺伝子組換え実験室, 准教授 (20159502)
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| Co-Investigator(Kenkyū-buntansha) |
ONIMARU Hiroshi 昭和大学, 医学部, 准教授 (30177258)
TAKETOMI Yoshitaka 昭和大学, 遺伝子組換え実験室, 助教 (40365804)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 病態モデル / PACAP / トランスジェニックマウス / 行動解析 / 発生工学 / 高次神経機能 / 行動生理 / PAC1 / 学習・記憶 / サーカディアン |
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| Research Abstract |
To define the physiological roles of pituitary adenylate cyclase-activating polypeptide (PACAP), we developed the PACAP-preferring receptor (PAC1) transgenic (Tg) mice using the Cre/loxP recombination system. The three type of PAC1 Tg mice which were expressed the Pac1 transgene in the whole body (wPAC1 Tg mice), in the hippocampus (hPAC1 Tg mice), and in the hippocampus after tamoxifen treatment (ihPAC1 Tg mice), were obtained.
We studied here the physiological roles of PACAP in brain using these PAC1 Tg mice. The spontaneous motor activity in home cage, the PAC1 expression, especially in hippocampus, increased behavioral activity at early dark period (ZT 12-17) which is most active time zone of the wild type mice. Furthermore, on the aged-mice which generally depressed motor activity, hPAC1 Tg mice showed more active with a clockwork circadian rhythm. These results suggest that the expression of PAC1 transgene, especially in the hippocampus nerve cells, make to enhance the behavioral activity with regular circadian rhythm. On the other hand, we studied the effect of PACAP in developmental respiratory adaptation by whole-body plethysmography and ex vivo analysis using brainstem-spinal cord preparation. Although this study is primitive, up to now, it was suggested that PACAP signaling concerned the development of central respiratory rhythmgensis.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Arata SDSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death.2009
Author(s)
Amano K, Fujii M, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg JR, Arata A, Yamakawa K.
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Journal Title
J Neurosci. 29(9)
Pages: 2984-96
Related Report
Peer Reviewed
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[Journal Article] Arata SDepletion of selenoprotein GPx4 in spermatocytes causes male infertility in mice.2009
Author(s)
Imai H, Hakkaku N, Iwamoto R, Suzuki J, Suzuki T, Tajima Y, Konishi K, Minami S, Ichinose S, Ishiizaka K, Shioda S, Nishimura M, Naito S, Nakagawa Y.
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Journal Title
J Biol Chem. 284(47)
Pages: 32522-32
Related Report
Peer Reviewed
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[Journal Article] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice.2008
Author(s)
Kageyama, H, Kitamura, Y, Hosono, T, Kintaka, Y, Seki, M, Takenoya, F, Hori, Y, Nonaka, N, Arata, S, Shioda, S
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Journal Title
Regul Pept. 145
Pages: 116-21
Related Report
Peer Reviewed
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[Journal Article] Arata S Generation of muscle aquaporin 4 overexpressing transgenic mouse: Its characterization at RNA and protein levels including freeze-fracture study.2007
Author(s)
Wakayama Y, Takahashi J, Shibuya S, Inoue M, Kojima H, Oniki H, Hara H, Jimi T, Shioda S, Sunada Y, Ohi H, Shimizu T.
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Journal Title
Micron. Micron. 38(3)
Pages: 257-267
Related Report
Peer Reviewed
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[Journal Article] Impaired mast cell maturation and degranulation and attenuated allergic responses in Ndrg1-deficient mice.2007
Author(s)
Taketomi YTanaka S, Sunaga K,, Nakamura M, Arata S, Okuda T, Moon TC, Chang HW, Sugimoto Y, Kokame K, Miyata T, Murakami M, Kudo I.
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Journal Title
J Immunol. 178(11)
Pages: 7042-7042
Related Report
Peer Reviewed
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[Presentation] Loss of Pre-inspiratory neuron synchroneity in mice with Dscam1 deficiency.2007
Author(s)
Amano K, Fujii M, Tojima T, Ogawa M, Morita N, Arata S, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, J R Korenberg, Arata A, Yamakawa K
Organizer
Neuroscience 2007
Place of Presentation
San Diego
Related Report
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