A role for post-translational modification of PPAR-gamma in vivo

• Project/Area Number: 19500372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokushima Bunri University
• Principal Investigator: OHSHIMA Takayuki Tokushima Bunri University, 香川薬学部, 准教授 (10397557)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 転写制御 / 翻訳後修飾 / 生活習慣病 / 細胞分化 / SUMO化修飾

Research Abstract

研究成果の概要:糖尿病などに代表される生活習慣病の最重要原因は肥満であり、核内受容体型転写制御因子PPAR-gamma は、脂肪細胞の分化制御を介した肥満の倹約因子である。今回我々は、PPAR-gamma のSUMO 化修飾の生理学的意義を明らかにすることを目的に、まずPPAR-gamma2 特異的ノックダウンマウスを作成した。そしてこのマウスは、痩せの大食いと共に、脂肪肝が全く認められないという表現系を示した。

Research Products

• [Journal Article] PIAS1 mediates TGFb-induced SMa-actin gene expression through inhibition of KLF4 function-expression by protein sumoylation (2009)
  • Author(s): Keiko Kawai-Kowase, Takayuki Ohshima, Hiroki Matsui, Toru Tanaka, Takahisa Shimizu,Tatsuya Iso, Masashi Arai, Gary K. Owens, Masahiko Kurabayashi
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 29 Pages: 99-106
  • Peer Reviewed
• [Journal Article] Sumoylation of CoREST modulates its function as a transcriptional repressor (2008)
  • Author(s): Ayako Muraoka, Atsuyo Maeda, Norie Nakahara, Midori Yokota, Tamotsu Nishida, Tokumi Maruyama, and Takayuki Ohshima
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 1031-1035
  • Peer Reviewed
• [Journal Article] HTLV-1 HBZ protein bypasses the targeting function of ubiquitination (2008)
  • Author(s): Osamu Isono, Takayuki Ohshima, ら他5名
  • Journal Title: J. Biol. Chem. 283 Pages: 34273-34282
  • Peer Reviewed
• [Journal Article] Synthesis and evaliation of 5'-modified 2'-deoxyadenosine analogues anti-hepatitis C agent (2008)
  • Author(s): Masahiro Ikejiri, Takayuki Ohshima, ら他3
  • Journal Title: Bioorg. Med. Chem. Letters 15 Pages: 4638-4641
  • Peer Reviewed
• [Journal Article] Sumoylation of CoREST modulates its function as a transcriptional repressor (2008)
  • Author(s): Ayako Muraoka, ら他6名
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 1031-1035
  • Peer Reviewed
• [Journal Article] BCL3 acts as a negative regulator of transcription from the HTLV-1 LTR through in teractions with TORC3 (2007)
  • Author(s): Takayuki Hishiki, Takayuki Ohshima, ら他2名
  • Journal Title: J.Biol.Chem 282 Pages: 28335-28343
  • Peer Reviewed
• [Journal Article] 5'-O-Masked 2'-deoxyadenosine analogues as lead compounds for hepatitis C virus(HCV)therapeutic agents (2007)
  • Author(s): Masahiro Ikejiri, Takayuki Ohshima, ら他2名
  • Journal Title: Bioorg.Med.Chem 15 Pages: 6882-6892
  • Peer Reviewed
• [Presentation] RNA干渉法を利用したPPAR-γ2特異的ノックダウンマウスの作出と機能解析 (2009)
  • Author(s): 大島隆幸, ら他3名
  • Organizer: 日本糖尿病・肥満動物学会
  • Place of Presentation: 岡山国際交流センター
  • Year and Date: 2009-02-13
• [Presentation] CoREST のSUMO 化修飾と神経特異的転写抑制機構 (2008)
  • Author(s): 村岡史子、前田敦代、丸山徳見、大島隆幸
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] CoRESTのSUMO化修飾と神経特異的転写抑制機構 (2007)
  • Author(s): 村岡 史子, ら他4名
  • Organizer: BMB2007
  • Place of Presentation: 横浜