Establishment of inducible basophil-less and eosinophil-less mouse lines to study critical roles in the IgE-mediated chronic allergic inflammation

• Project/Area Number: 19500374
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: MATSUOKA Kunie Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (40291158)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 病態モデル / 慢性アレルギー / 好塩基球 / 好酸球 / IgE / トランスジェニックマウス / ジフテリア毒素 / アレルギー / マウスモデル / 皮膚炎症

Research Abstract

ジフテリア毒素(DT)受容体を好塩基球あるいは好酸球に特異的に発現するトランスジェニックマウスを樹立した。DTの投与により末梢血中の好塩基球あるいは好酸球数が著明に減少し、抗原TNP-OVAの皮内投与で誘導されるIgE-Fc・RI依存性の慢性アレルギー反応による皮膚腫脹が軽減した。これらのマウスは好塩基球・好酸球の機能を解明する上で極めて有用であり、好塩基球・好酸球を標的とするアレルギーの治療法の開発に応用できる。

Research Products

• [Journal Article] The role of IgE and repeated challenge in the induction of persistent increase in scratching behavior in a mouse model of allergic dermatitis. (2009)
  • Author(s): Yamashita H., Tasaki D., Makino T., Matsuoka K., Nose M., Inagaki N., Mizukami H.
  • Journal Title: Eur. J. Pharmacol. 605 Pages: 153-157
  • Peer Reviewed
• [Journal Article] The role of IgE and repeated challenge in the induction of persistent increases in scratching behavior in a mouse model of allergic dermatitis (2009)
  • Author(s): Yamashita, H., Tasaki, D., Makino, T., Matsuoka, T., Nose, M., Inagaki, N., Mizukami, H.
  • Journal Title: Eur. J. Pharmacol. 605 Pages: 153-157
  • Peer Reviewed
• [Journal Article] A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis. (2008)
  • Author(s): Takada T., Shitara H., Matsuoka K., Kojima E., Ishii R., Kikkawa Y., Taya C., Karasuyama H., Kohno K., Yonekawa H.
  • Journal Title: Transgenic Res. 17 Pages: 1155-1162
  • Peer Reviewed
• [Journal Article] A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis (2008)
  • Author(s): Takada T., Shitara H., Matsuoka K., Kojima E., Ishii R., Kikkawa Y., Taya C., Karasuyama H., Kohno K., Yonekawa H.
  • Journal Title: Transgenic Res. 17 Pages: 1155-1162
  • Peer Reviewed
• [Presentation] 誘導型好酸球欠損マウスを用いたアレルギー反応の解析 (2008)
  • Author(s): 元田明日香、松岡邦枝、設楽浩志、飛田良美、松尾千古、河野憲二、小原道法、米川博通
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Establishment of an eosinophil-less mouse line by TRECK method. (2008)
  • Author(s): Motoda A., Shitara H., Kohno K., Yonekawa H. and Matsuoka K.
  • Organizer: 首都大学東京バイオコンファレンス2008
  • Place of Presentation: 東京
  • Year and Date: 2008-10-23
• [Presentation] Establishment of an eosinophil-less mouse line by TRECK method (2008)
  • Author(s): Motoda A., Shitara H., Kohno K., Yonekawa H., Masuoka K.
  • Organizer: 首都大学東京バイオコンファレンス2008
  • Place of Presentation: 東京(首都大学東京)
  • Year and Date: 2008-10-23
• [Presentation] Improvement of a tissue-specific gene expression for TRECK method using a BAC clone. (2008)
  • Author(s): Yonekawa H., Shibata K., Sekine M., Matsuoka K., Shitara H., Taya C. and. Amano T.
  • Organizer: The 22nd International Mammalian Genome Conference (IMGC 2008)
  • Place of Presentation: Prague, Czech
• [Presentation] Improvement of a tissue-specific gene expression for TRECK method using a BAC clone (2008)
  • Author(s): Yonekawa, H., Shibata, K., Sekine, M., Matsuoka, K., Shitara, H., Taya, C., Amano, T.
  • Organizer: The 22nd International Mammalian Genome Conference (IMGC2008)
  • Place of Presentation: プラハ(チェコ)
• [Presentation] 誘導型好酸球欠損マウスを用いたアレルギー反応の解析 (2008)
  • Author(s): 元田明日香, 松岡邦枝, 設楽浩志, 飛田良美, 松尾千古, 河野憲二, 小原道法, 米川博通
  • Organizer: BMB2008(第31回日本分子生物学会年会、第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸(国際会議場)
• [Presentation] 標的細胞ノックアウト(TRECK)法による誘導型好酸球欠損マウスの樹立とアレルギー反応の解析 (2007)
  • Author(s): 松岡邦枝、元田明日香、設楽浩志、河野憲二、米川博通
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会 (BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 標的細胞ノックアウト(TRECK)法による誘導型好酸球欠損マウスの樹立とアレルギー反応の解析 (2007)
  • Author(s): 松岡 邦枝
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会(BMB2007)
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2007-12-14
• [Presentation] Establishment of an eosinophil-less model line by TRECK method. (2007)
  • Author(s): Matsuoka K., Motoda A., Shitara H., Kohno K., Yonekawa H.
  • Organizer: 21st International Mammalian Genome Conference
  • Place of Presentation: Kyoto
  • Year and Date: 2007-10-30
• [Presentation] Establishment of an eosinophil-less mouse line by TRECK method. (2007)
  • Author(s): Kunie MATSUOKA
  • Organizer: 21st International Mammalian Genome Conference
  • Place of Presentation: 京都(京都テルサ)
  • Year and Date: 2007-10-30
• [Remarks]
  • URL: http://www.rinshoken.or.jp/LAS/index-jp.htm
• [Remarks]
  • URL: http://www.rinshoken.or.jp/LAS/index-jp.htm