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The study of origin tissue dependency of MSCs on chondrogenesis.

Research Project

Project/Area Number 19500403
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

ICHINOSE Shizuko  Tokyo Medical and Dental University, 先端研究支援センター, 助教 (60014156)

Co-Investigator(Kenkyū-buntansha) MUNETA Takeshi  東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (50190864)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords骨髄、滑膜、軟骨、脂肪組織由来間葉幹細胞 / 軟骨分化能 / 軟骨再生能 / 高密度培養 / コラーゲンゲル培養 / 移植実験 / 組織由来間葉幹細胞 / 軟骨分化
Research Abstract

本研究は、骨髄、滑膜、軟骨、脂肪組織由来間葉幹細胞の軟骨分化能の違いに関する実験を行うもので、骨髄、滑膜、軟骨、脂肪組織由来幹細胞の高密度培養とコラーゲンゲル培養により軟骨分化能を比較するin vitro実験(平成19年度)と骨髄、滑膜、軟骨、脂肪組織由来幹細胞-コラーゲンゲル複合体をウサギ軟骨欠損部およびラットの骨膜近傍に移植して軟骨分化能を比較するin vivo実験(平成20年度)から成っている。in vitro実験では、骨髄、滑膜、軟骨組織由来の軟骨分化誘導過程を比較し、論文および学会に発表した。in vivo実験では滑膜、骨髄組織由来の軟骨分化能を確認し、論文および学会に発表した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (35 results)

All 2010 2009 2008 2007

All Journal Article (26 results) (of which Peer Reviewed: 25 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Morphological differences during in vitro chondrogenesis of bone marrow-, synovium-MSCs, and chondrocytes2010

    • Author(s)
      Ichinose S, Muneta T, Koga H, Segawa Y, Tagami M, Tsuji K, Sekiya I.
    • Journal Title

      Lab Invest 90 (2)

      Pages: 210-21

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intra-articular Injected synovial stem cells differentiate into meniscal cells directly and promote meniscal regeneration without mobilization to distant organs in rat massive meniscal defect2009

    • Author(s)
      Horie M, Sekiya I, Muneta T, Ichinose S, Matsumoto K, Saito H, Murakami T, Kobayashi E.
    • Journal Title

      Stem Cells 27 (4)

      Pages: 878-87

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates2009

    • Author(s)
      Torigoe I, Sotome S, Tsuchiya A, Yoshii T, Maehara H, Sugata Y, Ichinose S, Shinomiya K, Okawa A.
    • Journal Title

      Tissue Eng Part A. 15 (7)

      Pages: 1489-99

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fresh bone marrow introduction into porous scaffolds using a simple low-pressure loading method for effective osteogenesis in a rabbit model2009

    • Author(s)
      Yoshii T, Sotome S, Torigoe I, Tsuchiya A, Maehara H, Ichinose S, Shinomiya K.
    • Journal Title

      J Orthop Res. 27 (1)

      Pages: 1-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bone Regeneration with Autologous Plasma, Bone Marrow StromalCells, and Porous beta-Tricalcium Phosphate in Nonhuman Primates.2009

    • Author(s)
      Torigoe I.
    • Journal Title

      Tissue Eng Part A. 3(Epub ahead of print)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fresh bone marrow introduction into porous scaffolds using a simplelow-pressure loading method for effective osteogenesis in a rabbit model.2009

    • Author(s)
      Yoshii T.
    • Journal Title

      J Orthop Res. 27

      Pages: 1-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exogenous synovial stem cells adhere to defect of meniscus and differentiate into cartilage cells2008

    • Author(s)
      Mizuno K, Muneta T, Morito T, Ichinose S, Koga H, Nimura A, Mochizuki T, Sekiya I.
    • Journal Title

      J Med Dent Sci. 55 (1)

      Pages: 101-11

    • NAID

      110006826918

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired meningeal development in association with apical expansion of calvarial bone osteogenesis in the Foxcl mutant2008

    • Author(s)
      Vivatbutsiri P, Ichinose S, Hytonen M, Sainio K, Eto K, Iseki S.
    • Journal Title

      J Anat 212 (5)

      Pages: 603-11

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired meningeal development in association with apical expansion of calvarial bone osteogenesis in the Foxcl mutant.2008

    • Author(s)
      Vivatbutsiri P.
    • Journal Title

      J Anat. 212

      Pages: 603-11

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired meningeal development in association with apical expansion of calvarial bone osteogenesis in the Foxcl2008

    • Author(s)
      Vivatbutsiri P
    • Journal Title

      J Anat. 212

      Pages: 603-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Statin-induced inhibition of HCV-1 release from latently infected U1 cells reveals a critical role for protein prenylatio2008

    • Author(s)
      Amet T
    • Journal Title

      Microbes Infect. (Epub ahead of)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hybridization quality in cervical cementum and superficial dentin using current adhesives.2008

    • Author(s)
      Yuan Y
    • Journal Title

      Dent Mater 24

      Pages: 584-93

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influences of mechanical properties and permeability on chitosan nano/microfiber mesh tubes as a scaffold for nerve2008

    • Author(s)
      Wang W
    • Journal Title

      J Biomed Mater Res 84

      Pages: 557-66

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological and immunohistochemical changes in muscle tissue in association with mandibular distraction osteogenesis2007

    • Author(s)
      Sato M, Maruoka Y, Kunimori K, Imai H, Kabasawa Y, Ichinose S, Harada K, Omura K.
    • Journal Title

      J Oral Maxillofac Surg 65 (8)

      Pages: 1517-25

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro formation of capillary networks using optical lithographic techniques2007

    • Author(s)
      Kobayashi A, Miyake H, Hattori H, Kuwana R, Hiruma Y, Nakahama K, Ichinose S, Ota M, Nakamura M, Takeda S, Morita I.
    • Journal Title

      Biochem Biophys Res Commun 358 (3)

      Pages: 692-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synovial stem cells are regionally specified according to local microenvironments after implantation for cartilage regeneration2007

    • Author(s)
      Koga H, Muneta T, Ju YJ, Nagase T, Nimura A, Mochizuki T, Ichinose S, von der Mark K, Sekiya I.
    • Journal Title

      Stem Cells 25 (3)

      Pages: 689-96

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト間葉幹細胞を用いた軟骨組織の再生ニューサイエンス社細胞2007

    • Author(s)
      市野瀬志津子, 田上幹樹, 宗田大, 関矢一郎
    • Journal Title

      細胞生物講座 39

      Pages: 311-315

    • Related Report
      2008 Final Research Report
  • [Journal Article] ヒト間葉幹細胞を用いた軟骨組織の再生2007

    • Author(s)
      市野瀬 志津子
    • Journal Title

      細胞生物講座 39

      Pages: 311-315

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro formation of capillary networks using optical lithographic techniques.2007

    • Author(s)
      Kobayashi A
    • Journal Title

      Biochem Biophys Res Commun 358

      Pages: 692-7

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synovial stem cells are regionally specified according to local microenvironments after implantation for cartilage rege2007

    • Author(s)
      Koga H
    • Journal Title

      Stem Cells. 25

      Pages: 689-96

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular distribution of mitochondrial ribosomal RNA in the mouse oocyte and zygote.2007

    • Author(s)
      Ninomiya Y
    • Journal Title

      PLoS ONE 28

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhaced nerve regeneration through a bilayered chitosan tube: The effect of introduction of glycine spacer into the2007

    • Author(s)
      Wang W
    • Journal Title

      J Biomed Mater Res (Epub ahead of)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological and immunohistochemical changes in muscle tissue in association with mandibular distraction osteoger2007

    • Author(s)
      Sato M
    • Journal Title

      J Oral Maxillofac Surg. 65

      Pages: 1517-25

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhalation of inorganic particles as a risk factor for idiopathic pulmonary fibrosis-Elemental microanalysis of pulmona2007

    • Author(s)
      Kitamura H
    • Journal Title

      Pathol Res Pract 203

      Pages: 575-85

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Scanning electron microscopy and energy-dispersive X-ray analysis of self-etching adhesive systems to ground and2007

    • Author(s)
      Weerasinghe DD
    • Journal Title

      J Mater Sci Mater Med. 18

      Pages: 1111-6

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Qualitative analysis of adhesive interface nanoleakage using FE-SEM/EDS.2007

    • Author(s)
      Yuan Y
    • Journal Title

      Dent Mater. 23

      Pages: 561-9

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 間葉幹細胞の生体内での挙動について2009

    • Author(s)
      市野瀬志津子, 井関祥子, 河村裕子, 田上幹樹
    • Organizer
      第41回日本臨床分子形態学会学術集会
    • Place of Presentation
      神戸市
    • Related Report
      2008 Final Research Report
  • [Presentation] コラーゲンゲル培養によるヒト骨髄由来間葉幹細胞の骨、軟骨組織の再生過程2008

    • Author(s)
      市野瀬志津子, 田上幹樹
    • Organizer
      第64回日本顕微鏡学会学術講演会
    • Place of Presentation
      京都市
    • Year and Date
      2008-05-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 間葉幹細胞の骨、軟骨分化過程の細胞接着について2008

    • Author(s)
      市野瀬志津子, 井関祥子, Philaiporn Vivatbutsiri, 河村裕子, 田上幹樹
    • Organizer
      第40回日本臨床分子形態学会学術集会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Final Research Report
  • [Presentation] コラーゲンゲル培養によるヒト骨髄由来間葉幹細胞の骨、軟骨組織の再生過程2008

    • Author(s)
      市野瀬 志津子
    • Organizer
      日本顕微鏡学会 第64回学術講演会
    • Place of Presentation
      京都市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 間葉幹細胞の骨、軟骨分化過程の細胞接着について2008

    • Author(s)
      市野瀬 志津子
    • Organizer
      第40回日本臨床分子形態学会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 由来組織の異なる間葉幹細胞の軟骨分化過程2007

    • Author(s)
      市野瀬志津子, 田上幹樹
    • Organizer
      第39回日本臨床分子形態学会学術集会
    • Place of Presentation
      甲府市
    • Related Report
      2008 Final Research Report
  • [Presentation] 由来組織の異なる間葉幹細胞の軟骨分化過程2007

    • Author(s)
      市野瀬 志津子
    • Organizer
      第39回日本臨床分子形態学会総会
    • Place of Presentation
      甲府市
    • Related Report
      2007 Annual Research Report
  • [Book] 第5章 免疫電顕法のコツ 市野瀬志津子2007

    • Author(s)
      免疫染色・イメージングのコツ, 井関祥子, 大田正人, 編
    • Publisher
      羊土社
    • Related Report
      2008 Final Research Report
  • [Book] 免疫染色・イメージングのコツ 第5章 免疫電顕法のコツ2007

    • Author(s)
      井関 祥子編, 市野瀬志津子
    • Publisher
      羊土社
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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